Targeted inhibition of long non-coding RNA H19 blocks anaplastic thyroid carcinoma growth and metastasis

Long non-coding RNA H19 (H19) is highly expressed in cancers and is considered to highly correlate with the extent of malignant degree. The present study was performed to determine the expression levels of H19 in anaplastic thyroid carcinoma (ATC) tissues and the role of H19 in ATC 8505C cells in vi...

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Autores principales: Zhang, Honglai, Yu, Ying, Zhang, Kejun, Liu, Xinfeng, Dai, Yan, Jiao, Xuelong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650201/
https://www.ncbi.nlm.nih.gov/pubmed/31299871
http://dx.doi.org/10.1080/21655979.2019.1642722
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author Zhang, Honglai
Yu, Ying
Zhang, Kejun
Liu, Xinfeng
Dai, Yan
Jiao, Xuelong
author_facet Zhang, Honglai
Yu, Ying
Zhang, Kejun
Liu, Xinfeng
Dai, Yan
Jiao, Xuelong
author_sort Zhang, Honglai
collection PubMed
description Long non-coding RNA H19 (H19) is highly expressed in cancers and is considered to highly correlate with the extent of malignant degree. The present study was performed to determine the expression levels of H19 in anaplastic thyroid carcinoma (ATC) tissues and the role of H19 in ATC 8505C cells in vitro and in vivo. Expression of H19 was detected in 19 ATC and 19 normal thyroid tissues by real-time quantitative polymerase chain reaction. Utilizing the siRNA or short hairpin RNA (shRNA) directed against human H19 (H19 siRNA or shRNA H19) depleted H19 in ATC 8505C cells and characterized the outcomes. The results showed that H19 was overexpressed in ATC tissues. Targeting H19 inhibited proliferation, migration, and invasion and induced apoptosis in 8505C cells in vitro and inhibited tumorigenesis and metastasis in vivo. Therefore, the H19 might be an effective target for ATC molecular therapy.
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spelling pubmed-66502012020-07-19 Targeted inhibition of long non-coding RNA H19 blocks anaplastic thyroid carcinoma growth and metastasis Zhang, Honglai Yu, Ying Zhang, Kejun Liu, Xinfeng Dai, Yan Jiao, Xuelong Bioengineered Research Paper Long non-coding RNA H19 (H19) is highly expressed in cancers and is considered to highly correlate with the extent of malignant degree. The present study was performed to determine the expression levels of H19 in anaplastic thyroid carcinoma (ATC) tissues and the role of H19 in ATC 8505C cells in vitro and in vivo. Expression of H19 was detected in 19 ATC and 19 normal thyroid tissues by real-time quantitative polymerase chain reaction. Utilizing the siRNA or short hairpin RNA (shRNA) directed against human H19 (H19 siRNA or shRNA H19) depleted H19 in ATC 8505C cells and characterized the outcomes. The results showed that H19 was overexpressed in ATC tissues. Targeting H19 inhibited proliferation, migration, and invasion and induced apoptosis in 8505C cells in vitro and inhibited tumorigenesis and metastasis in vivo. Therefore, the H19 might be an effective target for ATC molecular therapy. Taylor & Francis 2019-07-19 /pmc/articles/PMC6650201/ /pubmed/31299871 http://dx.doi.org/10.1080/21655979.2019.1642722 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Zhang, Honglai
Yu, Ying
Zhang, Kejun
Liu, Xinfeng
Dai, Yan
Jiao, Xuelong
Targeted inhibition of long non-coding RNA H19 blocks anaplastic thyroid carcinoma growth and metastasis
title Targeted inhibition of long non-coding RNA H19 blocks anaplastic thyroid carcinoma growth and metastasis
title_full Targeted inhibition of long non-coding RNA H19 blocks anaplastic thyroid carcinoma growth and metastasis
title_fullStr Targeted inhibition of long non-coding RNA H19 blocks anaplastic thyroid carcinoma growth and metastasis
title_full_unstemmed Targeted inhibition of long non-coding RNA H19 blocks anaplastic thyroid carcinoma growth and metastasis
title_short Targeted inhibition of long non-coding RNA H19 blocks anaplastic thyroid carcinoma growth and metastasis
title_sort targeted inhibition of long non-coding rna h19 blocks anaplastic thyroid carcinoma growth and metastasis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650201/
https://www.ncbi.nlm.nih.gov/pubmed/31299871
http://dx.doi.org/10.1080/21655979.2019.1642722
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