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Intestinal Preservation Injury: A Comparison Between Rat, Porcine and Human Intestines
Advanced preservation injury (PI) after intestinal transplantation has deleterious short- and long-term effects and constitutes a major research topic. Logistics and costs favor rodent studies, whereas clinical translation mandates studies in larger animals or using human material. Despite diverging...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650817/ https://www.ncbi.nlm.nih.gov/pubmed/31252560 http://dx.doi.org/10.3390/ijms20133135 |
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author | Søfteland, John Mackay Casselbrant, Anna Biglarnia, Ali-Reza Linders, Johan Hellström, Mats Pesce, Antonio Padma, Arvind Manikantan Jiga, Lucian Petru Hoinoiu, Bogdan Ionac, Mihai Oltean, Mihai |
author_facet | Søfteland, John Mackay Casselbrant, Anna Biglarnia, Ali-Reza Linders, Johan Hellström, Mats Pesce, Antonio Padma, Arvind Manikantan Jiga, Lucian Petru Hoinoiu, Bogdan Ionac, Mihai Oltean, Mihai |
author_sort | Søfteland, John Mackay |
collection | PubMed |
description | Advanced preservation injury (PI) after intestinal transplantation has deleterious short- and long-term effects and constitutes a major research topic. Logistics and costs favor rodent studies, whereas clinical translation mandates studies in larger animals or using human material. Despite diverging reports, no direct comparison between the development of intestinal PI in rats, pigs, and humans is available. We compared the development of PI in rat, porcine, and human intestines. Intestinal procurement and cold storage (CS) using histidine–tryptophan–ketoglutarate solution was performed in rats, pigs, and humans. Tissue samples were obtained after 8, 14, and 24 h of CS), and PI was assessed morphologically and at the molecular level (cleaved caspase-3, zonula occludens, claudin-3 and 4, tricellulin, occludin, cytokeratin-8) using immunohistochemistry and Western blot. Intestinal PI developed slower in pigs compared to rats and humans. Tissue injury and apoptosis were significantly higher in rats. Tight junction proteins showed quantitative and qualitative changes differing between species. Significant interspecies differences exist between rats, pigs, and humans regarding intestinal PI progression at tissue and molecular levels. These differences should be taken into account both with regards to study design and the interpretation of findings when relating them to the clinical setting. |
format | Online Article Text |
id | pubmed-6650817 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66508172019-08-07 Intestinal Preservation Injury: A Comparison Between Rat, Porcine and Human Intestines Søfteland, John Mackay Casselbrant, Anna Biglarnia, Ali-Reza Linders, Johan Hellström, Mats Pesce, Antonio Padma, Arvind Manikantan Jiga, Lucian Petru Hoinoiu, Bogdan Ionac, Mihai Oltean, Mihai Int J Mol Sci Article Advanced preservation injury (PI) after intestinal transplantation has deleterious short- and long-term effects and constitutes a major research topic. Logistics and costs favor rodent studies, whereas clinical translation mandates studies in larger animals or using human material. Despite diverging reports, no direct comparison between the development of intestinal PI in rats, pigs, and humans is available. We compared the development of PI in rat, porcine, and human intestines. Intestinal procurement and cold storage (CS) using histidine–tryptophan–ketoglutarate solution was performed in rats, pigs, and humans. Tissue samples were obtained after 8, 14, and 24 h of CS), and PI was assessed morphologically and at the molecular level (cleaved caspase-3, zonula occludens, claudin-3 and 4, tricellulin, occludin, cytokeratin-8) using immunohistochemistry and Western blot. Intestinal PI developed slower in pigs compared to rats and humans. Tissue injury and apoptosis were significantly higher in rats. Tight junction proteins showed quantitative and qualitative changes differing between species. Significant interspecies differences exist between rats, pigs, and humans regarding intestinal PI progression at tissue and molecular levels. These differences should be taken into account both with regards to study design and the interpretation of findings when relating them to the clinical setting. MDPI 2019-06-27 /pmc/articles/PMC6650817/ /pubmed/31252560 http://dx.doi.org/10.3390/ijms20133135 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Søfteland, John Mackay Casselbrant, Anna Biglarnia, Ali-Reza Linders, Johan Hellström, Mats Pesce, Antonio Padma, Arvind Manikantan Jiga, Lucian Petru Hoinoiu, Bogdan Ionac, Mihai Oltean, Mihai Intestinal Preservation Injury: A Comparison Between Rat, Porcine and Human Intestines |
title | Intestinal Preservation Injury: A Comparison Between Rat, Porcine and Human Intestines |
title_full | Intestinal Preservation Injury: A Comparison Between Rat, Porcine and Human Intestines |
title_fullStr | Intestinal Preservation Injury: A Comparison Between Rat, Porcine and Human Intestines |
title_full_unstemmed | Intestinal Preservation Injury: A Comparison Between Rat, Porcine and Human Intestines |
title_short | Intestinal Preservation Injury: A Comparison Between Rat, Porcine and Human Intestines |
title_sort | intestinal preservation injury: a comparison between rat, porcine and human intestines |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650817/ https://www.ncbi.nlm.nih.gov/pubmed/31252560 http://dx.doi.org/10.3390/ijms20133135 |
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