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Intestinal Preservation Injury: A Comparison Between Rat, Porcine and Human Intestines

Advanced preservation injury (PI) after intestinal transplantation has deleterious short- and long-term effects and constitutes a major research topic. Logistics and costs favor rodent studies, whereas clinical translation mandates studies in larger animals or using human material. Despite diverging...

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Autores principales: Søfteland, John Mackay, Casselbrant, Anna, Biglarnia, Ali-Reza, Linders, Johan, Hellström, Mats, Pesce, Antonio, Padma, Arvind Manikantan, Jiga, Lucian Petru, Hoinoiu, Bogdan, Ionac, Mihai, Oltean, Mihai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650817/
https://www.ncbi.nlm.nih.gov/pubmed/31252560
http://dx.doi.org/10.3390/ijms20133135
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author Søfteland, John Mackay
Casselbrant, Anna
Biglarnia, Ali-Reza
Linders, Johan
Hellström, Mats
Pesce, Antonio
Padma, Arvind Manikantan
Jiga, Lucian Petru
Hoinoiu, Bogdan
Ionac, Mihai
Oltean, Mihai
author_facet Søfteland, John Mackay
Casselbrant, Anna
Biglarnia, Ali-Reza
Linders, Johan
Hellström, Mats
Pesce, Antonio
Padma, Arvind Manikantan
Jiga, Lucian Petru
Hoinoiu, Bogdan
Ionac, Mihai
Oltean, Mihai
author_sort Søfteland, John Mackay
collection PubMed
description Advanced preservation injury (PI) after intestinal transplantation has deleterious short- and long-term effects and constitutes a major research topic. Logistics and costs favor rodent studies, whereas clinical translation mandates studies in larger animals or using human material. Despite diverging reports, no direct comparison between the development of intestinal PI in rats, pigs, and humans is available. We compared the development of PI in rat, porcine, and human intestines. Intestinal procurement and cold storage (CS) using histidine–tryptophan–ketoglutarate solution was performed in rats, pigs, and humans. Tissue samples were obtained after 8, 14, and 24 h of CS), and PI was assessed morphologically and at the molecular level (cleaved caspase-3, zonula occludens, claudin-3 and 4, tricellulin, occludin, cytokeratin-8) using immunohistochemistry and Western blot. Intestinal PI developed slower in pigs compared to rats and humans. Tissue injury and apoptosis were significantly higher in rats. Tight junction proteins showed quantitative and qualitative changes differing between species. Significant interspecies differences exist between rats, pigs, and humans regarding intestinal PI progression at tissue and molecular levels. These differences should be taken into account both with regards to study design and the interpretation of findings when relating them to the clinical setting.
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spelling pubmed-66508172019-08-07 Intestinal Preservation Injury: A Comparison Between Rat, Porcine and Human Intestines Søfteland, John Mackay Casselbrant, Anna Biglarnia, Ali-Reza Linders, Johan Hellström, Mats Pesce, Antonio Padma, Arvind Manikantan Jiga, Lucian Petru Hoinoiu, Bogdan Ionac, Mihai Oltean, Mihai Int J Mol Sci Article Advanced preservation injury (PI) after intestinal transplantation has deleterious short- and long-term effects and constitutes a major research topic. Logistics and costs favor rodent studies, whereas clinical translation mandates studies in larger animals or using human material. Despite diverging reports, no direct comparison between the development of intestinal PI in rats, pigs, and humans is available. We compared the development of PI in rat, porcine, and human intestines. Intestinal procurement and cold storage (CS) using histidine–tryptophan–ketoglutarate solution was performed in rats, pigs, and humans. Tissue samples were obtained after 8, 14, and 24 h of CS), and PI was assessed morphologically and at the molecular level (cleaved caspase-3, zonula occludens, claudin-3 and 4, tricellulin, occludin, cytokeratin-8) using immunohistochemistry and Western blot. Intestinal PI developed slower in pigs compared to rats and humans. Tissue injury and apoptosis were significantly higher in rats. Tight junction proteins showed quantitative and qualitative changes differing between species. Significant interspecies differences exist between rats, pigs, and humans regarding intestinal PI progression at tissue and molecular levels. These differences should be taken into account both with regards to study design and the interpretation of findings when relating them to the clinical setting. MDPI 2019-06-27 /pmc/articles/PMC6650817/ /pubmed/31252560 http://dx.doi.org/10.3390/ijms20133135 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Søfteland, John Mackay
Casselbrant, Anna
Biglarnia, Ali-Reza
Linders, Johan
Hellström, Mats
Pesce, Antonio
Padma, Arvind Manikantan
Jiga, Lucian Petru
Hoinoiu, Bogdan
Ionac, Mihai
Oltean, Mihai
Intestinal Preservation Injury: A Comparison Between Rat, Porcine and Human Intestines
title Intestinal Preservation Injury: A Comparison Between Rat, Porcine and Human Intestines
title_full Intestinal Preservation Injury: A Comparison Between Rat, Porcine and Human Intestines
title_fullStr Intestinal Preservation Injury: A Comparison Between Rat, Porcine and Human Intestines
title_full_unstemmed Intestinal Preservation Injury: A Comparison Between Rat, Porcine and Human Intestines
title_short Intestinal Preservation Injury: A Comparison Between Rat, Porcine and Human Intestines
title_sort intestinal preservation injury: a comparison between rat, porcine and human intestines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650817/
https://www.ncbi.nlm.nih.gov/pubmed/31252560
http://dx.doi.org/10.3390/ijms20133135
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