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Determination of the Role and Active Sites of PKC-Delta-Like from Lamprey in Innate Immunity

Protein kinase C-δ (PKC-δ) is an important protein in the immune system of higher vertebrates. Lampreys, as the most primitive vertebrates, have a uniquevariable lymphocyte receptor (VLR) immune system. PKC-δ-like is a crucial functional gene in lampreys and is highly expressed in their immune organ...

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Autores principales: Xu, Yang, Zhao, Huan, Tian, Yang, Ren, Kaixia, Zheng, Nan, Li, Qingwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650827/
https://www.ncbi.nlm.nih.gov/pubmed/31323909
http://dx.doi.org/10.3390/ijms20133357
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author Xu, Yang
Zhao, Huan
Tian, Yang
Ren, Kaixia
Zheng, Nan
Li, Qingwei
author_facet Xu, Yang
Zhao, Huan
Tian, Yang
Ren, Kaixia
Zheng, Nan
Li, Qingwei
author_sort Xu, Yang
collection PubMed
description Protein kinase C-δ (PKC-δ) is an important protein in the immune system of higher vertebrates. Lampreys, as the most primitive vertebrates, have a uniquevariable lymphocyte receptor (VLR) immune system. PKC-δ-like is a crucial functional gene in lampreys and is highly expressed in their immune organs. In this study, lampreys were stimulated with different immunogens, and lipopolysaccharide (LPS) was found to increase the expression of PKC-δ-like. Overexpression of PKC-δ-like could also effectively activate the innate immune response. We further demonstrated that PKC-δ-like-CF, a catalytic fragment of PKC-δ-like, is responsible for activating the innate immune response, and Thr-211, which is Thr-419 of PKC-δ-like, was confirmed to be the key site affecting PKC-δ-like-CF activity. These results indicated that PKC-δ-like from lamprey may have an important role in the innate immune response.
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spelling pubmed-66508272019-08-07 Determination of the Role and Active Sites of PKC-Delta-Like from Lamprey in Innate Immunity Xu, Yang Zhao, Huan Tian, Yang Ren, Kaixia Zheng, Nan Li, Qingwei Int J Mol Sci Communication Protein kinase C-δ (PKC-δ) is an important protein in the immune system of higher vertebrates. Lampreys, as the most primitive vertebrates, have a uniquevariable lymphocyte receptor (VLR) immune system. PKC-δ-like is a crucial functional gene in lampreys and is highly expressed in their immune organs. In this study, lampreys were stimulated with different immunogens, and lipopolysaccharide (LPS) was found to increase the expression of PKC-δ-like. Overexpression of PKC-δ-like could also effectively activate the innate immune response. We further demonstrated that PKC-δ-like-CF, a catalytic fragment of PKC-δ-like, is responsible for activating the innate immune response, and Thr-211, which is Thr-419 of PKC-δ-like, was confirmed to be the key site affecting PKC-δ-like-CF activity. These results indicated that PKC-δ-like from lamprey may have an important role in the innate immune response. MDPI 2019-07-09 /pmc/articles/PMC6650827/ /pubmed/31323909 http://dx.doi.org/10.3390/ijms20133357 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Xu, Yang
Zhao, Huan
Tian, Yang
Ren, Kaixia
Zheng, Nan
Li, Qingwei
Determination of the Role and Active Sites of PKC-Delta-Like from Lamprey in Innate Immunity
title Determination of the Role and Active Sites of PKC-Delta-Like from Lamprey in Innate Immunity
title_full Determination of the Role and Active Sites of PKC-Delta-Like from Lamprey in Innate Immunity
title_fullStr Determination of the Role and Active Sites of PKC-Delta-Like from Lamprey in Innate Immunity
title_full_unstemmed Determination of the Role and Active Sites of PKC-Delta-Like from Lamprey in Innate Immunity
title_short Determination of the Role and Active Sites of PKC-Delta-Like from Lamprey in Innate Immunity
title_sort determination of the role and active sites of pkc-delta-like from lamprey in innate immunity
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650827/
https://www.ncbi.nlm.nih.gov/pubmed/31323909
http://dx.doi.org/10.3390/ijms20133357
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