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Expression and Functional Analyses of Dlk1 in Muscle Stem Cells and Mesenchymal Progenitors during Muscle Regeneration

Delta like non-canonical Notch ligand 1 (Dlk1) is a paternally expressed gene which is also known as preadipocyte factor 1 (Pref−1). The accumulation of adipocytes and expression of Dlk1 in regenerating muscle suggests a correlation between fat accumulation and Dlk1 expression in the muscle. Additio...

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Autores principales: Zhang, Lidan, Uezumi, Akiyoshi, Kaji, Takayuki, Tsujikawa, Kazutake, Andersen, Ditte Caroline, Jensen, Charlotte Harken, Fukada, So-ichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650828/
https://www.ncbi.nlm.nih.gov/pubmed/31277245
http://dx.doi.org/10.3390/ijms20133269
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author Zhang, Lidan
Uezumi, Akiyoshi
Kaji, Takayuki
Tsujikawa, Kazutake
Andersen, Ditte Caroline
Jensen, Charlotte Harken
Fukada, So-ichiro
author_facet Zhang, Lidan
Uezumi, Akiyoshi
Kaji, Takayuki
Tsujikawa, Kazutake
Andersen, Ditte Caroline
Jensen, Charlotte Harken
Fukada, So-ichiro
author_sort Zhang, Lidan
collection PubMed
description Delta like non-canonical Notch ligand 1 (Dlk1) is a paternally expressed gene which is also known as preadipocyte factor 1 (Pref−1). The accumulation of adipocytes and expression of Dlk1 in regenerating muscle suggests a correlation between fat accumulation and Dlk1 expression in the muscle. Additionally, mice overexpressing Dlk1 show increased muscle weight, while Dlk1-null mice exhibit decreased body weight and muscle mass, indicating that Dlk1 is a critical factor in regulating skeletal muscle mass during development. The muscle regeneration process shares some features with muscle development. However, the role of Dlk1 in regeneration processes remains controversial. Here, we show that mesenchymal progenitors also known as adipocyte progenitors exclusively express Dlk1 during muscle regeneration. Eliminating developmental effects, we used conditional depletion models to examine the specific roles of Dlk1 in muscle stem cells or mesenchymal progenitors. Unexpectedly, deletion of Dlk1 in neither the muscle stem cells nor the mesenchymal progenitors affected the regenerative ability of skeletal muscle. In addition, fat accumulation was not increased by the loss of Dlk1. Collectively, Dlk1 plays essential roles in muscle development, but does not greatly impact regeneration processes and adipogenic differentiation in adult skeletal muscle regeneration.
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spelling pubmed-66508282019-08-07 Expression and Functional Analyses of Dlk1 in Muscle Stem Cells and Mesenchymal Progenitors during Muscle Regeneration Zhang, Lidan Uezumi, Akiyoshi Kaji, Takayuki Tsujikawa, Kazutake Andersen, Ditte Caroline Jensen, Charlotte Harken Fukada, So-ichiro Int J Mol Sci Article Delta like non-canonical Notch ligand 1 (Dlk1) is a paternally expressed gene which is also known as preadipocyte factor 1 (Pref−1). The accumulation of adipocytes and expression of Dlk1 in regenerating muscle suggests a correlation between fat accumulation and Dlk1 expression in the muscle. Additionally, mice overexpressing Dlk1 show increased muscle weight, while Dlk1-null mice exhibit decreased body weight and muscle mass, indicating that Dlk1 is a critical factor in regulating skeletal muscle mass during development. The muscle regeneration process shares some features with muscle development. However, the role of Dlk1 in regeneration processes remains controversial. Here, we show that mesenchymal progenitors also known as adipocyte progenitors exclusively express Dlk1 during muscle regeneration. Eliminating developmental effects, we used conditional depletion models to examine the specific roles of Dlk1 in muscle stem cells or mesenchymal progenitors. Unexpectedly, deletion of Dlk1 in neither the muscle stem cells nor the mesenchymal progenitors affected the regenerative ability of skeletal muscle. In addition, fat accumulation was not increased by the loss of Dlk1. Collectively, Dlk1 plays essential roles in muscle development, but does not greatly impact regeneration processes and adipogenic differentiation in adult skeletal muscle regeneration. MDPI 2019-07-03 /pmc/articles/PMC6650828/ /pubmed/31277245 http://dx.doi.org/10.3390/ijms20133269 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Lidan
Uezumi, Akiyoshi
Kaji, Takayuki
Tsujikawa, Kazutake
Andersen, Ditte Caroline
Jensen, Charlotte Harken
Fukada, So-ichiro
Expression and Functional Analyses of Dlk1 in Muscle Stem Cells and Mesenchymal Progenitors during Muscle Regeneration
title Expression and Functional Analyses of Dlk1 in Muscle Stem Cells and Mesenchymal Progenitors during Muscle Regeneration
title_full Expression and Functional Analyses of Dlk1 in Muscle Stem Cells and Mesenchymal Progenitors during Muscle Regeneration
title_fullStr Expression and Functional Analyses of Dlk1 in Muscle Stem Cells and Mesenchymal Progenitors during Muscle Regeneration
title_full_unstemmed Expression and Functional Analyses of Dlk1 in Muscle Stem Cells and Mesenchymal Progenitors during Muscle Regeneration
title_short Expression and Functional Analyses of Dlk1 in Muscle Stem Cells and Mesenchymal Progenitors during Muscle Regeneration
title_sort expression and functional analyses of dlk1 in muscle stem cells and mesenchymal progenitors during muscle regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650828/
https://www.ncbi.nlm.nih.gov/pubmed/31277245
http://dx.doi.org/10.3390/ijms20133269
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