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Influence of Electrode Connection Tracks on Biological Cell Measurements by Impedance Spectroscopy †

The limit of detection of a biological sensor is an important parameter because, when it is optimized, it allows the detection of a reduced number of biological cells and the reduction of the detection time. This parameter can be improved upon with a reduction in electrode size, but the rate of dete...

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Autores principales: Alves de Araujo, Arthur Luiz, Claudel, Julien, Kourtiche, Djilali, Nadi, Mustapha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650941/
https://www.ncbi.nlm.nih.gov/pubmed/31247894
http://dx.doi.org/10.3390/s19132839
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author Alves de Araujo, Arthur Luiz
Claudel, Julien
Kourtiche, Djilali
Nadi, Mustapha
author_facet Alves de Araujo, Arthur Luiz
Claudel, Julien
Kourtiche, Djilali
Nadi, Mustapha
author_sort Alves de Araujo, Arthur Luiz
collection PubMed
description The limit of detection of a biological sensor is an important parameter because, when it is optimized, it allows the detection of a reduced number of biological cells and the reduction of the detection time. This parameter can be improved upon with a reduction in electrode size, but the rate of detection is similarly reduced as well. To avoid this problem, we propose a sensor matrix composed of 20 × 20 µm² coplanar square electrodes with a standard clean room manufacturing process. However, it was observed that the exposition of electrode connection tracks to the solution reduces the normalized impedance variation. In this pursuit, we propose in this paper an analysis of electrode connection tracks on the normalized impedance variation and cutoff frequencies to biological cell measurements by impedance spectroscopy. The experimental results were obtained using the E4990A Keysight impedance analyser (Keysight Technologies, Santa Rosa, CA, USA) with a frequency band ranging from 100 Hz to 12 MHz, thus allowing for good measurement accuracy. Therefore, it was found that, for the measurements between the electrodes with 9 µm of connection tracks in contact with the solution, the normalized impedance variation was from 3.7% to 4.2% for different measurements, while, for the electrodes with 40 µm of connection tracks in contact with the solution, the normalized impedance variation was from 1.8% to 2.1% for different measurements.
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spelling pubmed-66509412019-08-07 Influence of Electrode Connection Tracks on Biological Cell Measurements by Impedance Spectroscopy † Alves de Araujo, Arthur Luiz Claudel, Julien Kourtiche, Djilali Nadi, Mustapha Sensors (Basel) Article The limit of detection of a biological sensor is an important parameter because, when it is optimized, it allows the detection of a reduced number of biological cells and the reduction of the detection time. This parameter can be improved upon with a reduction in electrode size, but the rate of detection is similarly reduced as well. To avoid this problem, we propose a sensor matrix composed of 20 × 20 µm² coplanar square electrodes with a standard clean room manufacturing process. However, it was observed that the exposition of electrode connection tracks to the solution reduces the normalized impedance variation. In this pursuit, we propose in this paper an analysis of electrode connection tracks on the normalized impedance variation and cutoff frequencies to biological cell measurements by impedance spectroscopy. The experimental results were obtained using the E4990A Keysight impedance analyser (Keysight Technologies, Santa Rosa, CA, USA) with a frequency band ranging from 100 Hz to 12 MHz, thus allowing for good measurement accuracy. Therefore, it was found that, for the measurements between the electrodes with 9 µm of connection tracks in contact with the solution, the normalized impedance variation was from 3.7% to 4.2% for different measurements, while, for the electrodes with 40 µm of connection tracks in contact with the solution, the normalized impedance variation was from 1.8% to 2.1% for different measurements. MDPI 2019-06-26 /pmc/articles/PMC6650941/ /pubmed/31247894 http://dx.doi.org/10.3390/s19132839 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alves de Araujo, Arthur Luiz
Claudel, Julien
Kourtiche, Djilali
Nadi, Mustapha
Influence of Electrode Connection Tracks on Biological Cell Measurements by Impedance Spectroscopy †
title Influence of Electrode Connection Tracks on Biological Cell Measurements by Impedance Spectroscopy †
title_full Influence of Electrode Connection Tracks on Biological Cell Measurements by Impedance Spectroscopy †
title_fullStr Influence of Electrode Connection Tracks on Biological Cell Measurements by Impedance Spectroscopy †
title_full_unstemmed Influence of Electrode Connection Tracks on Biological Cell Measurements by Impedance Spectroscopy †
title_short Influence of Electrode Connection Tracks on Biological Cell Measurements by Impedance Spectroscopy †
title_sort influence of electrode connection tracks on biological cell measurements by impedance spectroscopy †
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6650941/
https://www.ncbi.nlm.nih.gov/pubmed/31247894
http://dx.doi.org/10.3390/s19132839
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