Cargando…
Early Response of Radish to Heat Stress by Strand-Specific Transcriptome and miRNA Analysis
Radish is a crucial vegetable crop of the Brassicaceae family with many varieties and large cultivated area in China. Radish is a cool season crop, and there are only a few heat tolerant radish varieties in practical production with little information concerning the related genes in response to heat...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651063/ https://www.ncbi.nlm.nih.gov/pubmed/31284545 http://dx.doi.org/10.3390/ijms20133321 |
_version_ | 1783438258450989056 |
---|---|
author | Yang, Zhuang Li, Wen Su, Xiao Ge, Pingfei Zhou, Yan Hao, Yuanyuan Shu, Huangying Gao, Chonglun Cheng, Shanhan Zhu, Guopeng Wang, Zhiwei |
author_facet | Yang, Zhuang Li, Wen Su, Xiao Ge, Pingfei Zhou, Yan Hao, Yuanyuan Shu, Huangying Gao, Chonglun Cheng, Shanhan Zhu, Guopeng Wang, Zhiwei |
author_sort | Yang, Zhuang |
collection | PubMed |
description | Radish is a crucial vegetable crop of the Brassicaceae family with many varieties and large cultivated area in China. Radish is a cool season crop, and there are only a few heat tolerant radish varieties in practical production with little information concerning the related genes in response to heat stress. In this work, some physiological parameter changes of young leaves under short-term heat stress were detected. Furthermore, we acquired 1802 differentially expressed mRNAs (including encoding some heat shock proteins, heat shock factor and heat shock-related transcription factors), 169 differentially expressed lncRNAs and three differentially expressed circRNAs (novel_circ_0000265, novel_circ_0000325 and novel_circ_0000315) through strand-specific RNA sequencing technology. We also found 10 differentially expressed miRNAs (ath-miR159b-3p, athmiR159c, ath-miR398a-3p, athmiR398b-3p, ath-miR165a-5p, ath-miR169g-3p, novel_86, novel_107, novel_21 and ath-miR171b-3p) by small RNA sequencing technology. Through function prediction and enrichment analysis, our results suggested that the significantly possible pathways/complexes related to heat stress in radish leaves were circadian rhythm-plant, photosynthesis—antenna proteins, photosynthesis, carbon fixation in photosynthetic organisms, arginine and proline metabolism, oxidative phosphorylation, peroxisome and plant hormone signal transduction. Besides, we identified one lncRNA–miRNA–mRNAs combination responsive to heat stress. These results will be helpful for further illustration of molecular regulation networks of how radish responds to heat stress. |
format | Online Article Text |
id | pubmed-6651063 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66510632019-08-07 Early Response of Radish to Heat Stress by Strand-Specific Transcriptome and miRNA Analysis Yang, Zhuang Li, Wen Su, Xiao Ge, Pingfei Zhou, Yan Hao, Yuanyuan Shu, Huangying Gao, Chonglun Cheng, Shanhan Zhu, Guopeng Wang, Zhiwei Int J Mol Sci Article Radish is a crucial vegetable crop of the Brassicaceae family with many varieties and large cultivated area in China. Radish is a cool season crop, and there are only a few heat tolerant radish varieties in practical production with little information concerning the related genes in response to heat stress. In this work, some physiological parameter changes of young leaves under short-term heat stress were detected. Furthermore, we acquired 1802 differentially expressed mRNAs (including encoding some heat shock proteins, heat shock factor and heat shock-related transcription factors), 169 differentially expressed lncRNAs and three differentially expressed circRNAs (novel_circ_0000265, novel_circ_0000325 and novel_circ_0000315) through strand-specific RNA sequencing technology. We also found 10 differentially expressed miRNAs (ath-miR159b-3p, athmiR159c, ath-miR398a-3p, athmiR398b-3p, ath-miR165a-5p, ath-miR169g-3p, novel_86, novel_107, novel_21 and ath-miR171b-3p) by small RNA sequencing technology. Through function prediction and enrichment analysis, our results suggested that the significantly possible pathways/complexes related to heat stress in radish leaves were circadian rhythm-plant, photosynthesis—antenna proteins, photosynthesis, carbon fixation in photosynthetic organisms, arginine and proline metabolism, oxidative phosphorylation, peroxisome and plant hormone signal transduction. Besides, we identified one lncRNA–miRNA–mRNAs combination responsive to heat stress. These results will be helpful for further illustration of molecular regulation networks of how radish responds to heat stress. MDPI 2019-07-06 /pmc/articles/PMC6651063/ /pubmed/31284545 http://dx.doi.org/10.3390/ijms20133321 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Zhuang Li, Wen Su, Xiao Ge, Pingfei Zhou, Yan Hao, Yuanyuan Shu, Huangying Gao, Chonglun Cheng, Shanhan Zhu, Guopeng Wang, Zhiwei Early Response of Radish to Heat Stress by Strand-Specific Transcriptome and miRNA Analysis |
title | Early Response of Radish to Heat Stress by Strand-Specific Transcriptome and miRNA Analysis |
title_full | Early Response of Radish to Heat Stress by Strand-Specific Transcriptome and miRNA Analysis |
title_fullStr | Early Response of Radish to Heat Stress by Strand-Specific Transcriptome and miRNA Analysis |
title_full_unstemmed | Early Response of Radish to Heat Stress by Strand-Specific Transcriptome and miRNA Analysis |
title_short | Early Response of Radish to Heat Stress by Strand-Specific Transcriptome and miRNA Analysis |
title_sort | early response of radish to heat stress by strand-specific transcriptome and mirna analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651063/ https://www.ncbi.nlm.nih.gov/pubmed/31284545 http://dx.doi.org/10.3390/ijms20133321 |
work_keys_str_mv | AT yangzhuang earlyresponseofradishtoheatstressbystrandspecifictranscriptomeandmirnaanalysis AT liwen earlyresponseofradishtoheatstressbystrandspecifictranscriptomeandmirnaanalysis AT suxiao earlyresponseofradishtoheatstressbystrandspecifictranscriptomeandmirnaanalysis AT gepingfei earlyresponseofradishtoheatstressbystrandspecifictranscriptomeandmirnaanalysis AT zhouyan earlyresponseofradishtoheatstressbystrandspecifictranscriptomeandmirnaanalysis AT haoyuanyuan earlyresponseofradishtoheatstressbystrandspecifictranscriptomeandmirnaanalysis AT shuhuangying earlyresponseofradishtoheatstressbystrandspecifictranscriptomeandmirnaanalysis AT gaochonglun earlyresponseofradishtoheatstressbystrandspecifictranscriptomeandmirnaanalysis AT chengshanhan earlyresponseofradishtoheatstressbystrandspecifictranscriptomeandmirnaanalysis AT zhuguopeng earlyresponseofradishtoheatstressbystrandspecifictranscriptomeandmirnaanalysis AT wangzhiwei earlyresponseofradishtoheatstressbystrandspecifictranscriptomeandmirnaanalysis |