Cargando…

Night Shift Work, DNA Methylation and Telomere Length: An Investigation on Hospital Female Nurses

Increased breast cancer risk has been reported in some night shift (NS) workers but underlying biological mechanisms are still unclear. We assessed the association between NS work and DNA methylation of tumor suppressor (TP53, CDKN2A, BRCA1, BRCA2) and estrogen receptor (ESR1, ESR2) genes, methylati...

Descripción completa

Detalles Bibliográficos
Autores principales: Carugno, Michele, Maggioni, Cristina, Crespi, Eleonora, Bonzini, Matteo, Cuocina, Simone, Dioni, Laura, Tarantini, Letizia, Consonni, Dario, Ferrari, Luca, Pesatori, Angela Cecilia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651131/
https://www.ncbi.nlm.nih.gov/pubmed/31261650
http://dx.doi.org/10.3390/ijerph16132292
_version_ 1783438274285535232
author Carugno, Michele
Maggioni, Cristina
Crespi, Eleonora
Bonzini, Matteo
Cuocina, Simone
Dioni, Laura
Tarantini, Letizia
Consonni, Dario
Ferrari, Luca
Pesatori, Angela Cecilia
author_facet Carugno, Michele
Maggioni, Cristina
Crespi, Eleonora
Bonzini, Matteo
Cuocina, Simone
Dioni, Laura
Tarantini, Letizia
Consonni, Dario
Ferrari, Luca
Pesatori, Angela Cecilia
author_sort Carugno, Michele
collection PubMed
description Increased breast cancer risk has been reported in some night shift (NS) workers but underlying biological mechanisms are still unclear. We assessed the association between NS work and DNA methylation of tumor suppressor (TP53, CDKN2A, BRCA1, BRCA2) and estrogen receptor (ESR1, ESR2) genes, methylation of repetitive elements (LINE-1, Alu), and telomere length (TL). Forty six female nurses employed in NS for at least two years were matched by age (30–45 years) and length of service (≥1 year) with 51 female colleagues not working in NS. Each subject underwent a semi-structured interview and gave a blood sample. We applied linear regression and spline models adjusted for age, BMI, smoking habit, oral contraceptive use, parity and marital status/age at marriage. Currently working in NS was associated with ESR1 hypomethylation (β: −1.85 (95%CI: −3.03; −0.67), p = 0.003). In current and former NS workers we observed TP53 (−0.93 (−1.73; −0.12), p = 0.03) and BRCA1 (−1.14 (−1.71; −0.58), p <0.001) hypomethylation. We found an increase between TL and number of years in NS in subjects employed in NS <12 years (0.06 (0.03; 0.09), p <0.001), while a decrease if employed in NS ≥12 years (−0.07 −0.10; −0.04), p <0.001). Our findings show NS-associated markers potentially involved in cellular aging, genomic instability, and cancer development.
format Online
Article
Text
id pubmed-6651131
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-66511312019-08-07 Night Shift Work, DNA Methylation and Telomere Length: An Investigation on Hospital Female Nurses Carugno, Michele Maggioni, Cristina Crespi, Eleonora Bonzini, Matteo Cuocina, Simone Dioni, Laura Tarantini, Letizia Consonni, Dario Ferrari, Luca Pesatori, Angela Cecilia Int J Environ Res Public Health Article Increased breast cancer risk has been reported in some night shift (NS) workers but underlying biological mechanisms are still unclear. We assessed the association between NS work and DNA methylation of tumor suppressor (TP53, CDKN2A, BRCA1, BRCA2) and estrogen receptor (ESR1, ESR2) genes, methylation of repetitive elements (LINE-1, Alu), and telomere length (TL). Forty six female nurses employed in NS for at least two years were matched by age (30–45 years) and length of service (≥1 year) with 51 female colleagues not working in NS. Each subject underwent a semi-structured interview and gave a blood sample. We applied linear regression and spline models adjusted for age, BMI, smoking habit, oral contraceptive use, parity and marital status/age at marriage. Currently working in NS was associated with ESR1 hypomethylation (β: −1.85 (95%CI: −3.03; −0.67), p = 0.003). In current and former NS workers we observed TP53 (−0.93 (−1.73; −0.12), p = 0.03) and BRCA1 (−1.14 (−1.71; −0.58), p <0.001) hypomethylation. We found an increase between TL and number of years in NS in subjects employed in NS <12 years (0.06 (0.03; 0.09), p <0.001), while a decrease if employed in NS ≥12 years (−0.07 −0.10; −0.04), p <0.001). Our findings show NS-associated markers potentially involved in cellular aging, genomic instability, and cancer development. MDPI 2019-06-28 2019-07 /pmc/articles/PMC6651131/ /pubmed/31261650 http://dx.doi.org/10.3390/ijerph16132292 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Carugno, Michele
Maggioni, Cristina
Crespi, Eleonora
Bonzini, Matteo
Cuocina, Simone
Dioni, Laura
Tarantini, Letizia
Consonni, Dario
Ferrari, Luca
Pesatori, Angela Cecilia
Night Shift Work, DNA Methylation and Telomere Length: An Investigation on Hospital Female Nurses
title Night Shift Work, DNA Methylation and Telomere Length: An Investigation on Hospital Female Nurses
title_full Night Shift Work, DNA Methylation and Telomere Length: An Investigation on Hospital Female Nurses
title_fullStr Night Shift Work, DNA Methylation and Telomere Length: An Investigation on Hospital Female Nurses
title_full_unstemmed Night Shift Work, DNA Methylation and Telomere Length: An Investigation on Hospital Female Nurses
title_short Night Shift Work, DNA Methylation and Telomere Length: An Investigation on Hospital Female Nurses
title_sort night shift work, dna methylation and telomere length: an investigation on hospital female nurses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651131/
https://www.ncbi.nlm.nih.gov/pubmed/31261650
http://dx.doi.org/10.3390/ijerph16132292
work_keys_str_mv AT carugnomichele nightshiftworkdnamethylationandtelomerelengthaninvestigationonhospitalfemalenurses
AT maggionicristina nightshiftworkdnamethylationandtelomerelengthaninvestigationonhospitalfemalenurses
AT crespieleonora nightshiftworkdnamethylationandtelomerelengthaninvestigationonhospitalfemalenurses
AT bonzinimatteo nightshiftworkdnamethylationandtelomerelengthaninvestigationonhospitalfemalenurses
AT cuocinasimone nightshiftworkdnamethylationandtelomerelengthaninvestigationonhospitalfemalenurses
AT dionilaura nightshiftworkdnamethylationandtelomerelengthaninvestigationonhospitalfemalenurses
AT tarantiniletizia nightshiftworkdnamethylationandtelomerelengthaninvestigationonhospitalfemalenurses
AT consonnidario nightshiftworkdnamethylationandtelomerelengthaninvestigationonhospitalfemalenurses
AT ferrariluca nightshiftworkdnamethylationandtelomerelengthaninvestigationonhospitalfemalenurses
AT pesatoriangelacecilia nightshiftworkdnamethylationandtelomerelengthaninvestigationonhospitalfemalenurses