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Marek’s Disease Virus Infection Induced Mitochondria Changes in Chickens

Mitochondria are crucial cellular organelles in eukaryotes and participate in many cell processes including immune response, growth development, and tumorigenesis. Marek’s disease (MD), caused by an avian alpha-herpesvirus Marek’s disease virus (MDV), is characterized with lymphomas and immunosuppre...

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Autores principales: Chu, Qin, Ding, Yi, Cai, Wentao, Liu, Lei, Zhang, Huanmin, Song, Jiuzhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651546/
https://www.ncbi.nlm.nih.gov/pubmed/31252692
http://dx.doi.org/10.3390/ijms20133150
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author Chu, Qin
Ding, Yi
Cai, Wentao
Liu, Lei
Zhang, Huanmin
Song, Jiuzhou
author_facet Chu, Qin
Ding, Yi
Cai, Wentao
Liu, Lei
Zhang, Huanmin
Song, Jiuzhou
author_sort Chu, Qin
collection PubMed
description Mitochondria are crucial cellular organelles in eukaryotes and participate in many cell processes including immune response, growth development, and tumorigenesis. Marek’s disease (MD), caused by an avian alpha-herpesvirus Marek’s disease virus (MDV), is characterized with lymphomas and immunosuppression. In this research, we hypothesize that mitochondria may play roles in response to MDV infection. To test it, mitochondrial DNA (mtDNA) abundance and gene expression in immune organs were examined in two well-defined and highly inbred lines of chickens, the MD-susceptible line 7(2) and the MD-resistant line 6(3). We found that mitochondrial DNA contents decreased significantly at the transformation phase in spleen of the MD-susceptible line 7(2) birds in contrast to the MD-resistant line 6(3). The mtDNA-genes and the nucleus-genes relevant to mtDNA maintenance and transcription, however, were significantly up-regulated. Interestingly, we found that POLG2 might play a potential role that led to the imbalance of mtDNA copy number and gene expression alteration. MDV infection induced imbalance of mitochondrial contents and gene expression, demonstrating the indispensability of mitochondria in virus-induced cell transformation and subsequent lymphoma formation, such as MD development in chicken. This is the first report on relationship between virus infection and mitochondria in chicken, which provides important insights into the understanding on pathogenesis and tumorigenesis due to viral infection.
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spelling pubmed-66515462019-08-08 Marek’s Disease Virus Infection Induced Mitochondria Changes in Chickens Chu, Qin Ding, Yi Cai, Wentao Liu, Lei Zhang, Huanmin Song, Jiuzhou Int J Mol Sci Article Mitochondria are crucial cellular organelles in eukaryotes and participate in many cell processes including immune response, growth development, and tumorigenesis. Marek’s disease (MD), caused by an avian alpha-herpesvirus Marek’s disease virus (MDV), is characterized with lymphomas and immunosuppression. In this research, we hypothesize that mitochondria may play roles in response to MDV infection. To test it, mitochondrial DNA (mtDNA) abundance and gene expression in immune organs were examined in two well-defined and highly inbred lines of chickens, the MD-susceptible line 7(2) and the MD-resistant line 6(3). We found that mitochondrial DNA contents decreased significantly at the transformation phase in spleen of the MD-susceptible line 7(2) birds in contrast to the MD-resistant line 6(3). The mtDNA-genes and the nucleus-genes relevant to mtDNA maintenance and transcription, however, were significantly up-regulated. Interestingly, we found that POLG2 might play a potential role that led to the imbalance of mtDNA copy number and gene expression alteration. MDV infection induced imbalance of mitochondrial contents and gene expression, demonstrating the indispensability of mitochondria in virus-induced cell transformation and subsequent lymphoma formation, such as MD development in chicken. This is the first report on relationship between virus infection and mitochondria in chicken, which provides important insights into the understanding on pathogenesis and tumorigenesis due to viral infection. MDPI 2019-06-27 /pmc/articles/PMC6651546/ /pubmed/31252692 http://dx.doi.org/10.3390/ijms20133150 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chu, Qin
Ding, Yi
Cai, Wentao
Liu, Lei
Zhang, Huanmin
Song, Jiuzhou
Marek’s Disease Virus Infection Induced Mitochondria Changes in Chickens
title Marek’s Disease Virus Infection Induced Mitochondria Changes in Chickens
title_full Marek’s Disease Virus Infection Induced Mitochondria Changes in Chickens
title_fullStr Marek’s Disease Virus Infection Induced Mitochondria Changes in Chickens
title_full_unstemmed Marek’s Disease Virus Infection Induced Mitochondria Changes in Chickens
title_short Marek’s Disease Virus Infection Induced Mitochondria Changes in Chickens
title_sort marek’s disease virus infection induced mitochondria changes in chickens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651546/
https://www.ncbi.nlm.nih.gov/pubmed/31252692
http://dx.doi.org/10.3390/ijms20133150
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