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Magnetic Particles for Advanced Molecular Diagnosis

Molecular diagnosis is the field that aims to develop nucleic-acid-based analytical methods for biological markers and gene expression assessments by combining laboratory medicine and molecular genetics. As it gradually becomes a clinical reality, molecular diagnosis could benefit from improvements...

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Detalles Bibliográficos
Autores principales: Chircov, Cristina, Grumezescu, Alexandru Mihai, Holban, Alina Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651565/
https://www.ncbi.nlm.nih.gov/pubmed/31284393
http://dx.doi.org/10.3390/ma12132158
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author Chircov, Cristina
Grumezescu, Alexandru Mihai
Holban, Alina Maria
author_facet Chircov, Cristina
Grumezescu, Alexandru Mihai
Holban, Alina Maria
author_sort Chircov, Cristina
collection PubMed
description Molecular diagnosis is the field that aims to develop nucleic-acid-based analytical methods for biological markers and gene expression assessments by combining laboratory medicine and molecular genetics. As it gradually becomes a clinical reality, molecular diagnosis could benefit from improvements resulting from thorough studies that could enhance the accuracy of these methods. The application of magnetic particles in molecular diagnosis tools has led to tremendous breakthroughs in terms of specificity, sensitivity, and discrimination in bioassays. Therefore, the aim of this review is to highlight the principles involved in the implementation of magnetic particles for sample preparation and targeted analyte isolation, purification, and extraction. Furthermore, the most recent advancements in the area of cancer and infectious disease diagnosis are presented, with an emphasis on screening and early stage detection.
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spelling pubmed-66515652019-08-08 Magnetic Particles for Advanced Molecular Diagnosis Chircov, Cristina Grumezescu, Alexandru Mihai Holban, Alina Maria Materials (Basel) Review Molecular diagnosis is the field that aims to develop nucleic-acid-based analytical methods for biological markers and gene expression assessments by combining laboratory medicine and molecular genetics. As it gradually becomes a clinical reality, molecular diagnosis could benefit from improvements resulting from thorough studies that could enhance the accuracy of these methods. The application of magnetic particles in molecular diagnosis tools has led to tremendous breakthroughs in terms of specificity, sensitivity, and discrimination in bioassays. Therefore, the aim of this review is to highlight the principles involved in the implementation of magnetic particles for sample preparation and targeted analyte isolation, purification, and extraction. Furthermore, the most recent advancements in the area of cancer and infectious disease diagnosis are presented, with an emphasis on screening and early stage detection. MDPI 2019-07-05 /pmc/articles/PMC6651565/ /pubmed/31284393 http://dx.doi.org/10.3390/ma12132158 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Chircov, Cristina
Grumezescu, Alexandru Mihai
Holban, Alina Maria
Magnetic Particles for Advanced Molecular Diagnosis
title Magnetic Particles for Advanced Molecular Diagnosis
title_full Magnetic Particles for Advanced Molecular Diagnosis
title_fullStr Magnetic Particles for Advanced Molecular Diagnosis
title_full_unstemmed Magnetic Particles for Advanced Molecular Diagnosis
title_short Magnetic Particles for Advanced Molecular Diagnosis
title_sort magnetic particles for advanced molecular diagnosis
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651565/
https://www.ncbi.nlm.nih.gov/pubmed/31284393
http://dx.doi.org/10.3390/ma12132158
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