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Potential role of exercise-induced glucose-6-phosphate isomerase in skeletal muscle function

[PURPOSE]: Recent studies have shown that glucose-6-phosphate isomerase (GPI)—which is a glycolysis interconversion enzyme—reduces oxidative stress. However, these studies are limited to tumors such as fibrosarcoma, and there are no studies that have examined the effects of exercise on GPI expressio...

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Autores principales: Kwak, Seong Eun, Shin, Hyung Eun, Zhang, Di Di, Lee, Jihyun, Yoon, Kyung Jin, Bae, Jun Hyun, Moon, Hyo Youl, Song, Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 한국운동영양학회 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651688/
https://www.ncbi.nlm.nih.gov/pubmed/31337203
http://dx.doi.org/10.20463/jenb.2019.0014
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author Kwak, Seong Eun
Shin, Hyung Eun
Zhang, Di Di
Lee, Jihyun
Yoon, Kyung Jin
Bae, Jun Hyun
Moon, Hyo Youl
Song, Wook
author_facet Kwak, Seong Eun
Shin, Hyung Eun
Zhang, Di Di
Lee, Jihyun
Yoon, Kyung Jin
Bae, Jun Hyun
Moon, Hyo Youl
Song, Wook
author_sort Kwak, Seong Eun
collection PubMed
description [PURPOSE]: Recent studies have shown that glucose-6-phosphate isomerase (GPI)—which is a glycolysis interconversion enzyme—reduces oxidative stress. However, these studies are limited to tumors such as fibrosarcoma, and there are no studies that have examined the effects of exercise on GPI expression in mice skeletal muscle. Furthermore, GPI acts in an autocrine manner thorough its receptor, autocrine motility factor receptor (AMFR); therefore, we investigated expression level changes of secreted GPI from skeletal muscle in in vitro study to examine the potential role of GPI on skeletal muscle. [METHODS]: First, we performed an in vitro study, to identify the condition that upregulates GPI levels in skeletal muscle cells; we treated C2C12 muscle cells with an exercise-mimicking chemical, AICAR. AICAR treatment upregulated GPI expression level in C2C12 cell and its secretomes. To confirm the direct effect of GPI on skeletal muscle cells, we treated C2C12 cells with GPI recombinant protein. [RESULTS]: We found that GPI improved the viability of C2C12 cells. In the in vivo study, the exercise-treated mice group showed upregulated GPI expression in skeletal muscle. Based on the in vitro study results, we speculated that expression level of GPI in skeletal muscle might be associated with muscle function. We analyzed the association between GPI expression level and the grip strength of the all mice group. The mice group’s grip strengths were upregulated after 2 weeks of treadmill exercise, and GPI expression level positively correlated with the grip strength. [CONCLUSION]: These results suggested that the exercise-induced GPI expression in skeletal muscle might have a positive effect on skeletal muscle function.
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spelling pubmed-66516882019-07-29 Potential role of exercise-induced glucose-6-phosphate isomerase in skeletal muscle function Kwak, Seong Eun Shin, Hyung Eun Zhang, Di Di Lee, Jihyun Yoon, Kyung Jin Bae, Jun Hyun Moon, Hyo Youl Song, Wook J Exerc Nutrition Biochem Original Articles [PURPOSE]: Recent studies have shown that glucose-6-phosphate isomerase (GPI)—which is a glycolysis interconversion enzyme—reduces oxidative stress. However, these studies are limited to tumors such as fibrosarcoma, and there are no studies that have examined the effects of exercise on GPI expression in mice skeletal muscle. Furthermore, GPI acts in an autocrine manner thorough its receptor, autocrine motility factor receptor (AMFR); therefore, we investigated expression level changes of secreted GPI from skeletal muscle in in vitro study to examine the potential role of GPI on skeletal muscle. [METHODS]: First, we performed an in vitro study, to identify the condition that upregulates GPI levels in skeletal muscle cells; we treated C2C12 muscle cells with an exercise-mimicking chemical, AICAR. AICAR treatment upregulated GPI expression level in C2C12 cell and its secretomes. To confirm the direct effect of GPI on skeletal muscle cells, we treated C2C12 cells with GPI recombinant protein. [RESULTS]: We found that GPI improved the viability of C2C12 cells. In the in vivo study, the exercise-treated mice group showed upregulated GPI expression in skeletal muscle. Based on the in vitro study results, we speculated that expression level of GPI in skeletal muscle might be associated with muscle function. We analyzed the association between GPI expression level and the grip strength of the all mice group. The mice group’s grip strengths were upregulated after 2 weeks of treadmill exercise, and GPI expression level positively correlated with the grip strength. [CONCLUSION]: These results suggested that the exercise-induced GPI expression in skeletal muscle might have a positive effect on skeletal muscle function. 한국운동영양학회 2019-06-30 /pmc/articles/PMC6651688/ /pubmed/31337203 http://dx.doi.org/10.20463/jenb.2019.0014 Text en ©2019 The Korean Society for Exercise Nutrition ©2019 Seong Eun Kwak et al.; License Journal of Exercise Nutrition and Biochemistry. This is an open access article distributed under the terms of the creative commons attribution license (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the orginal work is properly cited.
spellingShingle Original Articles
Kwak, Seong Eun
Shin, Hyung Eun
Zhang, Di Di
Lee, Jihyun
Yoon, Kyung Jin
Bae, Jun Hyun
Moon, Hyo Youl
Song, Wook
Potential role of exercise-induced glucose-6-phosphate isomerase in skeletal muscle function
title Potential role of exercise-induced glucose-6-phosphate isomerase in skeletal muscle function
title_full Potential role of exercise-induced glucose-6-phosphate isomerase in skeletal muscle function
title_fullStr Potential role of exercise-induced glucose-6-phosphate isomerase in skeletal muscle function
title_full_unstemmed Potential role of exercise-induced glucose-6-phosphate isomerase in skeletal muscle function
title_short Potential role of exercise-induced glucose-6-phosphate isomerase in skeletal muscle function
title_sort potential role of exercise-induced glucose-6-phosphate isomerase in skeletal muscle function
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651688/
https://www.ncbi.nlm.nih.gov/pubmed/31337203
http://dx.doi.org/10.20463/jenb.2019.0014
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