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Novel Salicylic Acid Analogs Induce a Potent Defense Response in Arabidopsis

The master regulator of salicylic acid (SA)-mediated plant defense, NPR1 (NONEXPRESSER OF PR GENES 1) and its paralogs NPR3 and NPR4, act as SA receptors. After the perception of a pathogen, plant cells produce SA in the chloroplast. In the presence of SA, NPR1 protein is reduced from oligomers to m...

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Autores principales: Palmer, Ian Arthur, Chen, Huan, Chen, Jian, Chang, Ming, Li, Min, Liu, Fengquan, Fu, Zheng Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651783/
https://www.ncbi.nlm.nih.gov/pubmed/31288496
http://dx.doi.org/10.3390/ijms20133356
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author Palmer, Ian Arthur
Chen, Huan
Chen, Jian
Chang, Ming
Li, Min
Liu, Fengquan
Fu, Zheng Qing
author_facet Palmer, Ian Arthur
Chen, Huan
Chen, Jian
Chang, Ming
Li, Min
Liu, Fengquan
Fu, Zheng Qing
author_sort Palmer, Ian Arthur
collection PubMed
description The master regulator of salicylic acid (SA)-mediated plant defense, NPR1 (NONEXPRESSER OF PR GENES 1) and its paralogs NPR3 and NPR4, act as SA receptors. After the perception of a pathogen, plant cells produce SA in the chloroplast. In the presence of SA, NPR1 protein is reduced from oligomers to monomers, and translocated into the nucleus. There, NPR1 binds to TGA, TCP, and WRKY transcription factors to induce expression of plant defense genes. A list of compounds structurally similar to SA was generated using ChemMine Tools and its Clustering Toolbox. Several of these analogs can induce SA-mediated defense and inhibit growth of Pseudomonas syringae in Arabidopsis. These analogs, when sprayed on Arabidopsis, can induce the accumulation of the master regulator of plant defense NPR1. In a yeast two-hybrid system, these analogs can strengthen the interactions among NPR proteins. We demonstrated that these analogs can induce the expression of the defense marker gene PR1. Furthermore, we hypothesized that these SA analogs could be potent tools against the citrus greening pathogen Candidatus liberibacter spp. In fact, our results suggest that the SA analogs we tested using Arabidopsis may also be effective for inducing a defense response in citrus. Several SA analogs consistently strengthened the interactions between citrus NPR1 and NPR3 proteins in a yeast two-hybrid system. In future assays, we plan to test whether these analogs avoid degradation by SA hydroxylases from plant pathogens. In future assays, we plan to test whether these analogs avoid degradation by SA hydroxylases from plant pathogens.
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spelling pubmed-66517832019-08-08 Novel Salicylic Acid Analogs Induce a Potent Defense Response in Arabidopsis Palmer, Ian Arthur Chen, Huan Chen, Jian Chang, Ming Li, Min Liu, Fengquan Fu, Zheng Qing Int J Mol Sci Article The master regulator of salicylic acid (SA)-mediated plant defense, NPR1 (NONEXPRESSER OF PR GENES 1) and its paralogs NPR3 and NPR4, act as SA receptors. After the perception of a pathogen, plant cells produce SA in the chloroplast. In the presence of SA, NPR1 protein is reduced from oligomers to monomers, and translocated into the nucleus. There, NPR1 binds to TGA, TCP, and WRKY transcription factors to induce expression of plant defense genes. A list of compounds structurally similar to SA was generated using ChemMine Tools and its Clustering Toolbox. Several of these analogs can induce SA-mediated defense and inhibit growth of Pseudomonas syringae in Arabidopsis. These analogs, when sprayed on Arabidopsis, can induce the accumulation of the master regulator of plant defense NPR1. In a yeast two-hybrid system, these analogs can strengthen the interactions among NPR proteins. We demonstrated that these analogs can induce the expression of the defense marker gene PR1. Furthermore, we hypothesized that these SA analogs could be potent tools against the citrus greening pathogen Candidatus liberibacter spp. In fact, our results suggest that the SA analogs we tested using Arabidopsis may also be effective for inducing a defense response in citrus. Several SA analogs consistently strengthened the interactions between citrus NPR1 and NPR3 proteins in a yeast two-hybrid system. In future assays, we plan to test whether these analogs avoid degradation by SA hydroxylases from plant pathogens. In future assays, we plan to test whether these analogs avoid degradation by SA hydroxylases from plant pathogens. MDPI 2019-07-08 /pmc/articles/PMC6651783/ /pubmed/31288496 http://dx.doi.org/10.3390/ijms20133356 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Palmer, Ian Arthur
Chen, Huan
Chen, Jian
Chang, Ming
Li, Min
Liu, Fengquan
Fu, Zheng Qing
Novel Salicylic Acid Analogs Induce a Potent Defense Response in Arabidopsis
title Novel Salicylic Acid Analogs Induce a Potent Defense Response in Arabidopsis
title_full Novel Salicylic Acid Analogs Induce a Potent Defense Response in Arabidopsis
title_fullStr Novel Salicylic Acid Analogs Induce a Potent Defense Response in Arabidopsis
title_full_unstemmed Novel Salicylic Acid Analogs Induce a Potent Defense Response in Arabidopsis
title_short Novel Salicylic Acid Analogs Induce a Potent Defense Response in Arabidopsis
title_sort novel salicylic acid analogs induce a potent defense response in arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651783/
https://www.ncbi.nlm.nih.gov/pubmed/31288496
http://dx.doi.org/10.3390/ijms20133356
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