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Picoliter Cuvette inside an Optical Fiber to Track Gold Nanoparticle Aggregation for Measurement of Biomolecules
This study demonstrated a measurement approach for biomolecules at the picoliter scale, using a newly developed picoliter cuvette inside an optical fiber constructed via near-ultraviolet femtosecond laser drilling. The sensing capacity was estimated to be within 0.4–1.2 pL due to an optical path len...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651878/ https://www.ncbi.nlm.nih.gov/pubmed/31252623 http://dx.doi.org/10.3390/s19132859 |
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author | Shiraishi, Masahiko Watanabe, Kazuhiro Kubodera, Shoichi |
author_facet | Shiraishi, Masahiko Watanabe, Kazuhiro Kubodera, Shoichi |
author_sort | Shiraishi, Masahiko |
collection | PubMed |
description | This study demonstrated a measurement approach for biomolecules at the picoliter scale, using a newly developed picoliter cuvette inside an optical fiber constructed via near-ultraviolet femtosecond laser drilling. The sensing capacity was estimated to be within 0.4–1.2 pL due to an optical path length of 3–5 microns, as measured by scanning electron microscopy (SEM). The picoliter cuvette exhibited a change in the optical extinction spectrum after addition of biomolecules such as L-cysteine, in conjunction with a gold nanoparticle (GNP) dispersion solution, following a simple measurement configuration involving a small white light source and a compact spectrometer. A linear attenuation of the spectral dip near a wavelength of 520 nm was observed as the L-cysteine concentration was increased at 4 wt% of the GNP mass concentration. The measurement resolution of the concentration using the picoliter cuvette was evaluated at 0.125 mM. The experimental results showed the difference in aggregation processes caused by a different concentration of GNPs. Moreover, they revealed the ability of the picoliter cuvette to verify whether the concentration of GNPs in the liquid sample correspondingly determines homogeneous or inhomogeneous GNP aggregation, as supported by SEM observation and numerical calculations based on Mie theory. |
format | Online Article Text |
id | pubmed-6651878 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-66518782019-08-08 Picoliter Cuvette inside an Optical Fiber to Track Gold Nanoparticle Aggregation for Measurement of Biomolecules Shiraishi, Masahiko Watanabe, Kazuhiro Kubodera, Shoichi Sensors (Basel) Article This study demonstrated a measurement approach for biomolecules at the picoliter scale, using a newly developed picoliter cuvette inside an optical fiber constructed via near-ultraviolet femtosecond laser drilling. The sensing capacity was estimated to be within 0.4–1.2 pL due to an optical path length of 3–5 microns, as measured by scanning electron microscopy (SEM). The picoliter cuvette exhibited a change in the optical extinction spectrum after addition of biomolecules such as L-cysteine, in conjunction with a gold nanoparticle (GNP) dispersion solution, following a simple measurement configuration involving a small white light source and a compact spectrometer. A linear attenuation of the spectral dip near a wavelength of 520 nm was observed as the L-cysteine concentration was increased at 4 wt% of the GNP mass concentration. The measurement resolution of the concentration using the picoliter cuvette was evaluated at 0.125 mM. The experimental results showed the difference in aggregation processes caused by a different concentration of GNPs. Moreover, they revealed the ability of the picoliter cuvette to verify whether the concentration of GNPs in the liquid sample correspondingly determines homogeneous or inhomogeneous GNP aggregation, as supported by SEM observation and numerical calculations based on Mie theory. MDPI 2019-06-27 /pmc/articles/PMC6651878/ /pubmed/31252623 http://dx.doi.org/10.3390/s19132859 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shiraishi, Masahiko Watanabe, Kazuhiro Kubodera, Shoichi Picoliter Cuvette inside an Optical Fiber to Track Gold Nanoparticle Aggregation for Measurement of Biomolecules |
title | Picoliter Cuvette inside an Optical Fiber to Track Gold Nanoparticle Aggregation for Measurement of Biomolecules |
title_full | Picoliter Cuvette inside an Optical Fiber to Track Gold Nanoparticle Aggregation for Measurement of Biomolecules |
title_fullStr | Picoliter Cuvette inside an Optical Fiber to Track Gold Nanoparticle Aggregation for Measurement of Biomolecules |
title_full_unstemmed | Picoliter Cuvette inside an Optical Fiber to Track Gold Nanoparticle Aggregation for Measurement of Biomolecules |
title_short | Picoliter Cuvette inside an Optical Fiber to Track Gold Nanoparticle Aggregation for Measurement of Biomolecules |
title_sort | picoliter cuvette inside an optical fiber to track gold nanoparticle aggregation for measurement of biomolecules |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651878/ https://www.ncbi.nlm.nih.gov/pubmed/31252623 http://dx.doi.org/10.3390/s19132859 |
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