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Picoliter Cuvette inside an Optical Fiber to Track Gold Nanoparticle Aggregation for Measurement of Biomolecules

This study demonstrated a measurement approach for biomolecules at the picoliter scale, using a newly developed picoliter cuvette inside an optical fiber constructed via near-ultraviolet femtosecond laser drilling. The sensing capacity was estimated to be within 0.4–1.2 pL due to an optical path len...

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Autores principales: Shiraishi, Masahiko, Watanabe, Kazuhiro, Kubodera, Shoichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651878/
https://www.ncbi.nlm.nih.gov/pubmed/31252623
http://dx.doi.org/10.3390/s19132859
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author Shiraishi, Masahiko
Watanabe, Kazuhiro
Kubodera, Shoichi
author_facet Shiraishi, Masahiko
Watanabe, Kazuhiro
Kubodera, Shoichi
author_sort Shiraishi, Masahiko
collection PubMed
description This study demonstrated a measurement approach for biomolecules at the picoliter scale, using a newly developed picoliter cuvette inside an optical fiber constructed via near-ultraviolet femtosecond laser drilling. The sensing capacity was estimated to be within 0.4–1.2 pL due to an optical path length of 3–5 microns, as measured by scanning electron microscopy (SEM). The picoliter cuvette exhibited a change in the optical extinction spectrum after addition of biomolecules such as L-cysteine, in conjunction with a gold nanoparticle (GNP) dispersion solution, following a simple measurement configuration involving a small white light source and a compact spectrometer. A linear attenuation of the spectral dip near a wavelength of 520 nm was observed as the L-cysteine concentration was increased at 4 wt% of the GNP mass concentration. The measurement resolution of the concentration using the picoliter cuvette was evaluated at 0.125 mM. The experimental results showed the difference in aggregation processes caused by a different concentration of GNPs. Moreover, they revealed the ability of the picoliter cuvette to verify whether the concentration of GNPs in the liquid sample correspondingly determines homogeneous or inhomogeneous GNP aggregation, as supported by SEM observation and numerical calculations based on Mie theory.
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spelling pubmed-66518782019-08-08 Picoliter Cuvette inside an Optical Fiber to Track Gold Nanoparticle Aggregation for Measurement of Biomolecules Shiraishi, Masahiko Watanabe, Kazuhiro Kubodera, Shoichi Sensors (Basel) Article This study demonstrated a measurement approach for biomolecules at the picoliter scale, using a newly developed picoliter cuvette inside an optical fiber constructed via near-ultraviolet femtosecond laser drilling. The sensing capacity was estimated to be within 0.4–1.2 pL due to an optical path length of 3–5 microns, as measured by scanning electron microscopy (SEM). The picoliter cuvette exhibited a change in the optical extinction spectrum after addition of biomolecules such as L-cysteine, in conjunction with a gold nanoparticle (GNP) dispersion solution, following a simple measurement configuration involving a small white light source and a compact spectrometer. A linear attenuation of the spectral dip near a wavelength of 520 nm was observed as the L-cysteine concentration was increased at 4 wt% of the GNP mass concentration. The measurement resolution of the concentration using the picoliter cuvette was evaluated at 0.125 mM. The experimental results showed the difference in aggregation processes caused by a different concentration of GNPs. Moreover, they revealed the ability of the picoliter cuvette to verify whether the concentration of GNPs in the liquid sample correspondingly determines homogeneous or inhomogeneous GNP aggregation, as supported by SEM observation and numerical calculations based on Mie theory. MDPI 2019-06-27 /pmc/articles/PMC6651878/ /pubmed/31252623 http://dx.doi.org/10.3390/s19132859 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shiraishi, Masahiko
Watanabe, Kazuhiro
Kubodera, Shoichi
Picoliter Cuvette inside an Optical Fiber to Track Gold Nanoparticle Aggregation for Measurement of Biomolecules
title Picoliter Cuvette inside an Optical Fiber to Track Gold Nanoparticle Aggregation for Measurement of Biomolecules
title_full Picoliter Cuvette inside an Optical Fiber to Track Gold Nanoparticle Aggregation for Measurement of Biomolecules
title_fullStr Picoliter Cuvette inside an Optical Fiber to Track Gold Nanoparticle Aggregation for Measurement of Biomolecules
title_full_unstemmed Picoliter Cuvette inside an Optical Fiber to Track Gold Nanoparticle Aggregation for Measurement of Biomolecules
title_short Picoliter Cuvette inside an Optical Fiber to Track Gold Nanoparticle Aggregation for Measurement of Biomolecules
title_sort picoliter cuvette inside an optical fiber to track gold nanoparticle aggregation for measurement of biomolecules
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651878/
https://www.ncbi.nlm.nih.gov/pubmed/31252623
http://dx.doi.org/10.3390/s19132859
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