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Impact of sequencing depth and technology on de novo RNA-Seq assembly

BACKGROUND: RNA-Seq data is inherently nonuniform for different transcripts because of differences in gene expression. This makes it challenging to decide how much data should be generated from each sample. How much should one spend to recover the less expressed transcripts? The sequencing technolog...

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Detalles Bibliográficos
Autores principales:	Patterson, Jordan, Carpenter, Eric J., Zhu, Zhenzhen, An, Dan, Liang, Xinming, Geng, Chunyu, Drmanac, Radoje, Wong, Gane Ka-Shu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2019
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651908/ https://www.ncbi.nlm.nih.gov/pubmed/31337347 http://dx.doi.org/10.1186/s12864-019-5965-x

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