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Angiopoietin‐like protein 8 expression and association with extracellular matrix metabolism and inflammation during intervertebral disc degeneration

Intervertebral disc degeneration (IDD) is considered the primary culprit for low back pain. Although the underlying mechanisms remain unknown, hyperactive catabolism of the extracellular matrix (ECM) and inflammation are suggested to play critical roles in IDD progression. This study was designed to...

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Autores principales: Liao, Zhiwei, Wu, Xinghuo, Song, Yu, Luo, Rongjin, Yin, Huipeng, Zhan, Shengfeng, Li, Shuai, Wang, Kun, Zhang, Yukun, Yang, Cao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6653761/
https://www.ncbi.nlm.nih.gov/pubmed/31211513
http://dx.doi.org/10.1111/jcmm.14488
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author Liao, Zhiwei
Wu, Xinghuo
Song, Yu
Luo, Rongjin
Yin, Huipeng
Zhan, Shengfeng
Li, Shuai
Wang, Kun
Zhang, Yukun
Yang, Cao
author_facet Liao, Zhiwei
Wu, Xinghuo
Song, Yu
Luo, Rongjin
Yin, Huipeng
Zhan, Shengfeng
Li, Shuai
Wang, Kun
Zhang, Yukun
Yang, Cao
author_sort Liao, Zhiwei
collection PubMed
description Intervertebral disc degeneration (IDD) is considered the primary culprit for low back pain. Although the underlying mechanisms remain unknown, hyperactive catabolism of the extracellular matrix (ECM) and inflammation are suggested to play critical roles in IDD progression. This study was designed to elucidate the role of angiopoietin‐like protein 8 (ANGPTL8) in the progression of IDD, especially the relationship of ANGPTL8 with ECM metabolism and inflammation. A positive association between ANGPTL8 expression and degenerative grades of IDD was detected in the analysis of human nucleus pulposus tissue samples. Silencing of ANGPTL8 attenuated the degradation of the anabolic protein type collagen II, and reduced the expression of the catabolic proteins MMP3 and MMP9, and the inflammatory cytokine IL‐6 through inhibition of NF‐κB signalling activation. In addition, the effect of ANGPTL8 was evaluated in a rat model of puncture‐induced IDD. Based on the imaging results and histological examination in animal study, knockdown of ANGPTL8 was demonstrated to ameliorate the IDD progression. These results demonstrate the detrimental role of ANGPTL8 expression in the pathogenesis of IDD and may provide a new therapeutic target for IDD treatment.
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spelling pubmed-66537612019-08-01 Angiopoietin‐like protein 8 expression and association with extracellular matrix metabolism and inflammation during intervertebral disc degeneration Liao, Zhiwei Wu, Xinghuo Song, Yu Luo, Rongjin Yin, Huipeng Zhan, Shengfeng Li, Shuai Wang, Kun Zhang, Yukun Yang, Cao J Cell Mol Med Original Articles Intervertebral disc degeneration (IDD) is considered the primary culprit for low back pain. Although the underlying mechanisms remain unknown, hyperactive catabolism of the extracellular matrix (ECM) and inflammation are suggested to play critical roles in IDD progression. This study was designed to elucidate the role of angiopoietin‐like protein 8 (ANGPTL8) in the progression of IDD, especially the relationship of ANGPTL8 with ECM metabolism and inflammation. A positive association between ANGPTL8 expression and degenerative grades of IDD was detected in the analysis of human nucleus pulposus tissue samples. Silencing of ANGPTL8 attenuated the degradation of the anabolic protein type collagen II, and reduced the expression of the catabolic proteins MMP3 and MMP9, and the inflammatory cytokine IL‐6 through inhibition of NF‐κB signalling activation. In addition, the effect of ANGPTL8 was evaluated in a rat model of puncture‐induced IDD. Based on the imaging results and histological examination in animal study, knockdown of ANGPTL8 was demonstrated to ameliorate the IDD progression. These results demonstrate the detrimental role of ANGPTL8 expression in the pathogenesis of IDD and may provide a new therapeutic target for IDD treatment. John Wiley and Sons Inc. 2019-06-18 2019-08 /pmc/articles/PMC6653761/ /pubmed/31211513 http://dx.doi.org/10.1111/jcmm.14488 Text en © 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Liao, Zhiwei
Wu, Xinghuo
Song, Yu
Luo, Rongjin
Yin, Huipeng
Zhan, Shengfeng
Li, Shuai
Wang, Kun
Zhang, Yukun
Yang, Cao
Angiopoietin‐like protein 8 expression and association with extracellular matrix metabolism and inflammation during intervertebral disc degeneration
title Angiopoietin‐like protein 8 expression and association with extracellular matrix metabolism and inflammation during intervertebral disc degeneration
title_full Angiopoietin‐like protein 8 expression and association with extracellular matrix metabolism and inflammation during intervertebral disc degeneration
title_fullStr Angiopoietin‐like protein 8 expression and association with extracellular matrix metabolism and inflammation during intervertebral disc degeneration
title_full_unstemmed Angiopoietin‐like protein 8 expression and association with extracellular matrix metabolism and inflammation during intervertebral disc degeneration
title_short Angiopoietin‐like protein 8 expression and association with extracellular matrix metabolism and inflammation during intervertebral disc degeneration
title_sort angiopoietin‐like protein 8 expression and association with extracellular matrix metabolism and inflammation during intervertebral disc degeneration
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6653761/
https://www.ncbi.nlm.nih.gov/pubmed/31211513
http://dx.doi.org/10.1111/jcmm.14488
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