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Imbalance of antioxidant enzymes activities and trace elements levels in Ghanaian HIV-infected patients

Human immunodeficiency virus (HIV) infection and antiretroviral therapy (ART) have been associated with high oxidative stress in HIV patients. The disparity in antioxidant-oxidant levels in HIV patients favours viral replication and disease progression. This study aimed at determining the effect of...

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Autores principales: Quaye, Osbourne, Kuleape, Joshua Agbemefa, Bonney, Evelyn Yayra, Puplampu, Peter, Tagoe, Emmanuel Ayitey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6655767/
https://www.ncbi.nlm.nih.gov/pubmed/31339937
http://dx.doi.org/10.1371/journal.pone.0220181
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author Quaye, Osbourne
Kuleape, Joshua Agbemefa
Bonney, Evelyn Yayra
Puplampu, Peter
Tagoe, Emmanuel Ayitey
author_facet Quaye, Osbourne
Kuleape, Joshua Agbemefa
Bonney, Evelyn Yayra
Puplampu, Peter
Tagoe, Emmanuel Ayitey
author_sort Quaye, Osbourne
collection PubMed
description Human immunodeficiency virus (HIV) infection and antiretroviral therapy (ART) have been associated with high oxidative stress in HIV patients. The disparity in antioxidant-oxidant levels in HIV patients favours viral replication and disease progression. This study aimed at determining the effect of ART on antioxidant enzymes activities and trace elements levels in Ghanaian HIV patients. A total of 242 participants; comprising of 105 HIV-infected patients on ART, 77 HIV-infected ART-naïve, and 60 HIV seronegative controls were recruited for the study. Whole blood was collected and used for haematological profiling, and the determination of CD4(+) counts, superoxide dismutase (SOD) activity and trace element levels. Serum was used for liver function tests and the determination of glutathione reductase (GR) activity, and plasma was used to estimate reduced glutathione (GSH) levels. Low levels of haemoglobin (HB), hematocrit, mean cell volume (MCV) and mean cell hemoglobin (MCH), and trace elements were found in ART-naïve patients compared to those on ART and the seronegative controls. In the ART-naïve patients, glutathione reductase (GR) activity and reduced glutathione (GSH) level were significantly low compared to patients on ART and seronegative controls. Activity of SOD was significantly reduced in ART-naïve patients compared to those on ART and the control group, and manganese is the only trace element that showed a strong negative correlation with SOD activity and a positive and significant correlation with CD4(+) count, and therefore needs to be investigated further. The study suggests that assessing antioxidant levels or enzymes activities of patients infected with HIV should be considered during therapy.
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spelling pubmed-66557672019-08-07 Imbalance of antioxidant enzymes activities and trace elements levels in Ghanaian HIV-infected patients Quaye, Osbourne Kuleape, Joshua Agbemefa Bonney, Evelyn Yayra Puplampu, Peter Tagoe, Emmanuel Ayitey PLoS One Research Article Human immunodeficiency virus (HIV) infection and antiretroviral therapy (ART) have been associated with high oxidative stress in HIV patients. The disparity in antioxidant-oxidant levels in HIV patients favours viral replication and disease progression. This study aimed at determining the effect of ART on antioxidant enzymes activities and trace elements levels in Ghanaian HIV patients. A total of 242 participants; comprising of 105 HIV-infected patients on ART, 77 HIV-infected ART-naïve, and 60 HIV seronegative controls were recruited for the study. Whole blood was collected and used for haematological profiling, and the determination of CD4(+) counts, superoxide dismutase (SOD) activity and trace element levels. Serum was used for liver function tests and the determination of glutathione reductase (GR) activity, and plasma was used to estimate reduced glutathione (GSH) levels. Low levels of haemoglobin (HB), hematocrit, mean cell volume (MCV) and mean cell hemoglobin (MCH), and trace elements were found in ART-naïve patients compared to those on ART and the seronegative controls. In the ART-naïve patients, glutathione reductase (GR) activity and reduced glutathione (GSH) level were significantly low compared to patients on ART and seronegative controls. Activity of SOD was significantly reduced in ART-naïve patients compared to those on ART and the control group, and manganese is the only trace element that showed a strong negative correlation with SOD activity and a positive and significant correlation with CD4(+) count, and therefore needs to be investigated further. The study suggests that assessing antioxidant levels or enzymes activities of patients infected with HIV should be considered during therapy. Public Library of Science 2019-07-24 /pmc/articles/PMC6655767/ /pubmed/31339937 http://dx.doi.org/10.1371/journal.pone.0220181 Text en © 2019 Quaye et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Quaye, Osbourne
Kuleape, Joshua Agbemefa
Bonney, Evelyn Yayra
Puplampu, Peter
Tagoe, Emmanuel Ayitey
Imbalance of antioxidant enzymes activities and trace elements levels in Ghanaian HIV-infected patients
title Imbalance of antioxidant enzymes activities and trace elements levels in Ghanaian HIV-infected patients
title_full Imbalance of antioxidant enzymes activities and trace elements levels in Ghanaian HIV-infected patients
title_fullStr Imbalance of antioxidant enzymes activities and trace elements levels in Ghanaian HIV-infected patients
title_full_unstemmed Imbalance of antioxidant enzymes activities and trace elements levels in Ghanaian HIV-infected patients
title_short Imbalance of antioxidant enzymes activities and trace elements levels in Ghanaian HIV-infected patients
title_sort imbalance of antioxidant enzymes activities and trace elements levels in ghanaian hiv-infected patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6655767/
https://www.ncbi.nlm.nih.gov/pubmed/31339937
http://dx.doi.org/10.1371/journal.pone.0220181
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