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Epidermal growth factor receptor rs17337023 polymorphism in hypertensive gestational diabetic women: A pilot study

BACKGROUND: Women with gestational diabetes mellitus have an increased risk of developing gestational hypertension, which can increase fetal and neonatal morbidity and mortality. In the past decade, single nucleotide polymorphisms in several genes have been identified as risk factors for development...

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Autores principales: Martins, Russell S, Ahmed, Taimur, Farhat, Sabah, Shahid, Sana, Fatima, Syeda Sadia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6656705/
https://www.ncbi.nlm.nih.gov/pubmed/31363386
http://dx.doi.org/10.4239/wjd.v10.i7.396
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author Martins, Russell S
Ahmed, Taimur
Farhat, Sabah
Shahid, Sana
Fatima, Syeda Sadia
author_facet Martins, Russell S
Ahmed, Taimur
Farhat, Sabah
Shahid, Sana
Fatima, Syeda Sadia
author_sort Martins, Russell S
collection PubMed
description BACKGROUND: Women with gestational diabetes mellitus have an increased risk of developing gestational hypertension, which can increase fetal and neonatal morbidity and mortality. In the past decade, single nucleotide polymorphisms in several genes have been identified as risk factors for development of gestational hypertension. The epidermal growth factor receptor activates tyrosine kinase mediated blood vessels contractility; and inflammatory cascades. Abnormalities in these mechanism are known to contribute towards hypertension. It is thus plausible that polymorphisms in the epidermal growth factor receptor gene would be associated with the development of hypertension in women with gestational diabetes. AIM: To determine whether the epidermal growth factor receptor rs17337023 SNP is associated with the occurrence of hypertension in gestational diabetic women. METHODS: This pilot case-control study was conducted at two tertiary care hospitals in Karachi, from January 2017-August 2018. Two hundred and two women at 28 week of gestation with gestational diabetes were recruited and classified into normotensive (n = 80) and hypertensive (n = 122) groups. Their blood samples were genotyped for epidermal growth factor receptor polymorphism rs17337023 using tetra-ARMS polymerase chain reaction. Descriptive analysis was applied on baseline data. Polymorphism data was analyzed for genotype and allele frequency determination using chi-squared statistics. In all cases, a P value of < 0.05 was considered significant. RESULTS: Subjects were age-matched and thus no difference was observed in relation to age of the study subjects (P >0.05). Body fat percentage was significantly higher in hypertensive females as compared to normotensive subjects (35.138 ± 4.29 Case vs 25.01 ± 8.28 Control; P < 0.05). Similarly, systolic and diastolic blood pressures among groups were significantly higher in hypertensive group than the normotensive group (P < 0.05). Overall epidermal growth factor receptor rs17337023 polymorphism genotype frequency was similar in both groups, with the heterozygous AT genotype (56 in Case vs 48 in Control; P = 0. 079) showing predominance in both groups. Furthermore, the odds ratio for A allele was 1.282 (P = 0.219) and for T allele was 0.780 (P = 0.221) in this study. CONCLUSION: This pilot study indicates that polymorphisms in rs17337023 may not be involved in the pathophysiology of gestational hypertension in gestational diabetes via inflammatory cascade mechanism. Further large-scale studies should explore polymorphism in epidermal growth factor receptor and other genes in this regard.
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spelling pubmed-66567052019-07-30 Epidermal growth factor receptor rs17337023 polymorphism in hypertensive gestational diabetic women: A pilot study Martins, Russell S Ahmed, Taimur Farhat, Sabah Shahid, Sana Fatima, Syeda Sadia World J Diabetes Basic Study BACKGROUND: Women with gestational diabetes mellitus have an increased risk of developing gestational hypertension, which can increase fetal and neonatal morbidity and mortality. In the past decade, single nucleotide polymorphisms in several genes have been identified as risk factors for development of gestational hypertension. The epidermal growth factor receptor activates tyrosine kinase mediated blood vessels contractility; and inflammatory cascades. Abnormalities in these mechanism are known to contribute towards hypertension. It is thus plausible that polymorphisms in the epidermal growth factor receptor gene would be associated with the development of hypertension in women with gestational diabetes. AIM: To determine whether the epidermal growth factor receptor rs17337023 SNP is associated with the occurrence of hypertension in gestational diabetic women. METHODS: This pilot case-control study was conducted at two tertiary care hospitals in Karachi, from January 2017-August 2018. Two hundred and two women at 28 week of gestation with gestational diabetes were recruited and classified into normotensive (n = 80) and hypertensive (n = 122) groups. Their blood samples were genotyped for epidermal growth factor receptor polymorphism rs17337023 using tetra-ARMS polymerase chain reaction. Descriptive analysis was applied on baseline data. Polymorphism data was analyzed for genotype and allele frequency determination using chi-squared statistics. In all cases, a P value of < 0.05 was considered significant. RESULTS: Subjects were age-matched and thus no difference was observed in relation to age of the study subjects (P >0.05). Body fat percentage was significantly higher in hypertensive females as compared to normotensive subjects (35.138 ± 4.29 Case vs 25.01 ± 8.28 Control; P < 0.05). Similarly, systolic and diastolic blood pressures among groups were significantly higher in hypertensive group than the normotensive group (P < 0.05). Overall epidermal growth factor receptor rs17337023 polymorphism genotype frequency was similar in both groups, with the heterozygous AT genotype (56 in Case vs 48 in Control; P = 0. 079) showing predominance in both groups. Furthermore, the odds ratio for A allele was 1.282 (P = 0.219) and for T allele was 0.780 (P = 0.221) in this study. CONCLUSION: This pilot study indicates that polymorphisms in rs17337023 may not be involved in the pathophysiology of gestational hypertension in gestational diabetes via inflammatory cascade mechanism. Further large-scale studies should explore polymorphism in epidermal growth factor receptor and other genes in this regard. Baishideng Publishing Group Inc 2019-07-15 2019-07-15 /pmc/articles/PMC6656705/ /pubmed/31363386 http://dx.doi.org/10.4239/wjd.v10.i7.396 Text en ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.
spellingShingle Basic Study
Martins, Russell S
Ahmed, Taimur
Farhat, Sabah
Shahid, Sana
Fatima, Syeda Sadia
Epidermal growth factor receptor rs17337023 polymorphism in hypertensive gestational diabetic women: A pilot study
title Epidermal growth factor receptor rs17337023 polymorphism in hypertensive gestational diabetic women: A pilot study
title_full Epidermal growth factor receptor rs17337023 polymorphism in hypertensive gestational diabetic women: A pilot study
title_fullStr Epidermal growth factor receptor rs17337023 polymorphism in hypertensive gestational diabetic women: A pilot study
title_full_unstemmed Epidermal growth factor receptor rs17337023 polymorphism in hypertensive gestational diabetic women: A pilot study
title_short Epidermal growth factor receptor rs17337023 polymorphism in hypertensive gestational diabetic women: A pilot study
title_sort epidermal growth factor receptor rs17337023 polymorphism in hypertensive gestational diabetic women: a pilot study
topic Basic Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6656705/
https://www.ncbi.nlm.nih.gov/pubmed/31363386
http://dx.doi.org/10.4239/wjd.v10.i7.396
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