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Identification of bovine papillomavirus type 1 and 2 from bovine anogenital fibropapillomas

Papillomavirus (PV) is a well-known pathogen associated with epithelial and mucosal neoplastic diseases. In contrast to human PVs, characterization of animal PVs from the aspect of anogenital neoplasm is still on a learning curve. In the present study, two vulval and one anal warts, histologically d...

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Autores principales: YAMASHITA-KAWANISHI, Nanako, TSUZUKI, Masano, WEI, Zhu, KOK, Mun Keong, ISHIYAMA, Dai, CHAMBERS, James K., UCHIDA, Kazuyuki, DONG, Jianbao, SHIMAKURA, Hidekatsu, HAGA, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6656810/
https://www.ncbi.nlm.nih.gov/pubmed/31155549
http://dx.doi.org/10.1292/jvms.19-0017
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author YAMASHITA-KAWANISHI, Nanako
TSUZUKI, Masano
WEI, Zhu
KOK, Mun Keong
ISHIYAMA, Dai
CHAMBERS, James K.
UCHIDA, Kazuyuki
DONG, Jianbao
SHIMAKURA, Hidekatsu
HAGA, Takeshi
author_facet YAMASHITA-KAWANISHI, Nanako
TSUZUKI, Masano
WEI, Zhu
KOK, Mun Keong
ISHIYAMA, Dai
CHAMBERS, James K.
UCHIDA, Kazuyuki
DONG, Jianbao
SHIMAKURA, Hidekatsu
HAGA, Takeshi
author_sort YAMASHITA-KAWANISHI, Nanako
collection PubMed
description Papillomavirus (PV) is a well-known pathogen associated with epithelial and mucosal neoplastic diseases. In contrast to human PVs, characterization of animal PVs from the aspect of anogenital neoplasm is still on a learning curve. In the present study, two vulval and one anal warts, histologically diagnosed as fibropapillomas, excised from dairy cattle were analyzed. PCR and sequencing revealed that bovine papillomavirus type 1 (BPV-1) and BPV-2 were detected from anal and vulval fibropapillomas, respectively. Immunohistochemistry detected PV antigen in a few differentiated keratinocytes of one vulval case. Reverse-transcriptase PCR detected the early region, but not the late region of BPV mRNA in all three cases. The present study will provide new insight into the relationship between BPV and anogenital papilloma in cattle.
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spelling pubmed-66568102019-07-31 Identification of bovine papillomavirus type 1 and 2 from bovine anogenital fibropapillomas YAMASHITA-KAWANISHI, Nanako TSUZUKI, Masano WEI, Zhu KOK, Mun Keong ISHIYAMA, Dai CHAMBERS, James K. UCHIDA, Kazuyuki DONG, Jianbao SHIMAKURA, Hidekatsu HAGA, Takeshi J Vet Med Sci Clinical Pathology Papillomavirus (PV) is a well-known pathogen associated with epithelial and mucosal neoplastic diseases. In contrast to human PVs, characterization of animal PVs from the aspect of anogenital neoplasm is still on a learning curve. In the present study, two vulval and one anal warts, histologically diagnosed as fibropapillomas, excised from dairy cattle were analyzed. PCR and sequencing revealed that bovine papillomavirus type 1 (BPV-1) and BPV-2 were detected from anal and vulval fibropapillomas, respectively. Immunohistochemistry detected PV antigen in a few differentiated keratinocytes of one vulval case. Reverse-transcriptase PCR detected the early region, but not the late region of BPV mRNA in all three cases. The present study will provide new insight into the relationship between BPV and anogenital papilloma in cattle. The Japanese Society of Veterinary Science 2019-06-03 2019-07 /pmc/articles/PMC6656810/ /pubmed/31155549 http://dx.doi.org/10.1292/jvms.19-0017 Text en ©2019 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Clinical Pathology
YAMASHITA-KAWANISHI, Nanako
TSUZUKI, Masano
WEI, Zhu
KOK, Mun Keong
ISHIYAMA, Dai
CHAMBERS, James K.
UCHIDA, Kazuyuki
DONG, Jianbao
SHIMAKURA, Hidekatsu
HAGA, Takeshi
Identification of bovine papillomavirus type 1 and 2 from bovine anogenital fibropapillomas
title Identification of bovine papillomavirus type 1 and 2 from bovine anogenital fibropapillomas
title_full Identification of bovine papillomavirus type 1 and 2 from bovine anogenital fibropapillomas
title_fullStr Identification of bovine papillomavirus type 1 and 2 from bovine anogenital fibropapillomas
title_full_unstemmed Identification of bovine papillomavirus type 1 and 2 from bovine anogenital fibropapillomas
title_short Identification of bovine papillomavirus type 1 and 2 from bovine anogenital fibropapillomas
title_sort identification of bovine papillomavirus type 1 and 2 from bovine anogenital fibropapillomas
topic Clinical Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6656810/
https://www.ncbi.nlm.nih.gov/pubmed/31155549
http://dx.doi.org/10.1292/jvms.19-0017
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