One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus

To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one-step multiplex PCR/RT-PCR assay was developed, with detection limits of 10(2.1) TCID(50) for CDV, 10(1.9) TCID(50) for CPV and 10(3) copies for CaKoV. This method did not amp...

Descripción completa

Detalles Bibliográficos
Autores principales: LIU, Dafei, LIU, Fei, GUO, Dongchun, HU, Xiaoliang, LI, Zhijie, LI, Zhigang, MA, Jianzhang, LIU, Chunguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2018
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6656820/
https://www.ncbi.nlm.nih.gov/pubmed/29367517
http://dx.doi.org/10.1292/jvms.17-0442
_version_ 1783438695230078976
author LIU, Dafei
LIU, Fei
GUO, Dongchun
HU, Xiaoliang
LI, Zhijie
LI, Zhigang
MA, Jianzhang
LIU, Chunguo
author_facet LIU, Dafei
LIU, Fei
GUO, Dongchun
HU, Xiaoliang
LI, Zhijie
LI, Zhigang
MA, Jianzhang
LIU, Chunguo
author_sort LIU, Dafei
collection PubMed
description To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one-step multiplex PCR/RT-PCR assay was developed, with detection limits of 10(2.1) TCID(50) for CDV, 10(1.9) TCID(50) for CPV and 10(3) copies for CaKoV. This method did not amplify nonspecific DNA or RNA from other canine viruses. Therefore, the assay provides a sensitive tool for the rapid clinical detection and epidemiological surveillance of CDV, CPV and CaKoV in dogs.
format Online
Article
Text
id pubmed-6656820
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher The Japanese Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-66568202019-07-31 One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus LIU, Dafei LIU, Fei GUO, Dongchun HU, Xiaoliang LI, Zhijie LI, Zhigang MA, Jianzhang LIU, Chunguo J Vet Med Sci Virology To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one-step multiplex PCR/RT-PCR assay was developed, with detection limits of 10(2.1) TCID(50) for CDV, 10(1.9) TCID(50) for CPV and 10(3) copies for CaKoV. This method did not amplify nonspecific DNA or RNA from other canine viruses. Therefore, the assay provides a sensitive tool for the rapid clinical detection and epidemiological surveillance of CDV, CPV and CaKoV in dogs. The Japanese Society of Veterinary Science 2018-01-23 2019-07 /pmc/articles/PMC6656820/ /pubmed/29367517 http://dx.doi.org/10.1292/jvms.17-0442 Text en ©2019 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Virology
LIU, Dafei
LIU, Fei
GUO, Dongchun
HU, Xiaoliang
LI, Zhijie
LI, Zhigang
MA, Jianzhang
LIU, Chunguo
One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus
title One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus
title_full One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus
title_fullStr One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus
title_full_unstemmed One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus
title_short One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus
title_sort one-step triplex pcr/rt-pcr to detect canine distemper virus, canine parvovirus and canine kobuvirus
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6656820/
https://www.ncbi.nlm.nih.gov/pubmed/29367517
http://dx.doi.org/10.1292/jvms.17-0442
work_keys_str_mv AT liudafei onesteptriplexpcrrtpcrtodetectcaninedistemperviruscanineparvovirusandcaninekobuvirus
AT liufei onesteptriplexpcrrtpcrtodetectcaninedistemperviruscanineparvovirusandcaninekobuvirus
AT guodongchun onesteptriplexpcrrtpcrtodetectcaninedistemperviruscanineparvovirusandcaninekobuvirus
AT huxiaoliang onesteptriplexpcrrtpcrtodetectcaninedistemperviruscanineparvovirusandcaninekobuvirus
AT lizhijie onesteptriplexpcrrtpcrtodetectcaninedistemperviruscanineparvovirusandcaninekobuvirus
AT lizhigang onesteptriplexpcrrtpcrtodetectcaninedistemperviruscanineparvovirusandcaninekobuvirus
AT majianzhang onesteptriplexpcrrtpcrtodetectcaninedistemperviruscanineparvovirusandcaninekobuvirus
AT liuchunguo onesteptriplexpcrrtpcrtodetectcaninedistemperviruscanineparvovirusandcaninekobuvirus

Ejemplares similares