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One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus
To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one-step multiplex PCR/RT-PCR assay was developed, with detection limits of 10(2.1) TCID(50) for CDV, 10(1.9) TCID(50) for CPV and 10(3) copies for CaKoV. This method did not amp...
Autores principales: | LIU, Dafei, LIU, Fei, GUO, Dongchun, HU, Xiaoliang, LI, Zhijie, LI, Zhigang, MA, Jianzhang, LIU, Chunguo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6656820/ https://www.ncbi.nlm.nih.gov/pubmed/29367517 http://dx.doi.org/10.1292/jvms.17-0442 |
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