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Polycystic ovary syndrome dependency on mtDNA mutation; copy Number and its association with insulin resistance
OBJECTIVE: Study analyzes mutation in mtDNA (Mitochondrial DNA) among diabetic women with PCOS in non-diabetic diabetic women and compared with the healthy control. Women with known case of hyperandrogenism, ovulatory dysfunction and/or polycystic ovaries were selected and anthropometric and demogra...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6657173/ https://www.ncbi.nlm.nih.gov/pubmed/31340838 http://dx.doi.org/10.1186/s13104-019-4453-3 |
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author | Saeed, Noor AlHuda Ali A. H. Hamzah, Israa Hussein Al-Gharrawi, Samar Abdul Raheem |
author_facet | Saeed, Noor AlHuda Ali A. H. Hamzah, Israa Hussein Al-Gharrawi, Samar Abdul Raheem |
author_sort | Saeed, Noor AlHuda Ali A. H. |
collection | PubMed |
description | OBJECTIVE: Study analyzes mutation in mtDNA (Mitochondrial DNA) among diabetic women with PCOS in non-diabetic diabetic women and compared with the healthy control. Women with known case of hyperandrogenism, ovulatory dysfunction and/or polycystic ovaries were selected and anthropometric and demographic variables were collected during their clinical visit. Biochemical estimation of glucose, FSH, LH, estradiol (E2), and insulin levels were analyzed. Mutational analysis of mt-tRNA genes of each individual was compared with the updated consensus Cambridge sequence. The mtDNA content was determined in triplicate using SYBR green PCR mastermix. RESULTS: The clinical and biochemical characteristics of participants showed no statistical difference in age and/or FSH, PRL, E2, PRGE or fasting glucose value between patients of different groups. Women with PCOS-D had significantly higher LH, LH/FSH, TT and fasting insulin levels and HOMA-IR with respect to the control group. Ten different type of mutation were seen in POCS group. Most of these mutations were confined to evolutionarily conserved region. The mtDNA copy numbers were considerably lower PCOS group irrespective of diabetic status. To conclude, the current study inferred that the mutations occur in the mitochondrial genome, mt-tRNA in specific, are the important causal factor in PCOS. |
format | Online Article Text |
id | pubmed-6657173 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-66571732019-07-31 Polycystic ovary syndrome dependency on mtDNA mutation; copy Number and its association with insulin resistance Saeed, Noor AlHuda Ali A. H. Hamzah, Israa Hussein Al-Gharrawi, Samar Abdul Raheem BMC Res Notes Research Note OBJECTIVE: Study analyzes mutation in mtDNA (Mitochondrial DNA) among diabetic women with PCOS in non-diabetic diabetic women and compared with the healthy control. Women with known case of hyperandrogenism, ovulatory dysfunction and/or polycystic ovaries were selected and anthropometric and demographic variables were collected during their clinical visit. Biochemical estimation of glucose, FSH, LH, estradiol (E2), and insulin levels were analyzed. Mutational analysis of mt-tRNA genes of each individual was compared with the updated consensus Cambridge sequence. The mtDNA content was determined in triplicate using SYBR green PCR mastermix. RESULTS: The clinical and biochemical characteristics of participants showed no statistical difference in age and/or FSH, PRL, E2, PRGE or fasting glucose value between patients of different groups. Women with PCOS-D had significantly higher LH, LH/FSH, TT and fasting insulin levels and HOMA-IR with respect to the control group. Ten different type of mutation were seen in POCS group. Most of these mutations were confined to evolutionarily conserved region. The mtDNA copy numbers were considerably lower PCOS group irrespective of diabetic status. To conclude, the current study inferred that the mutations occur in the mitochondrial genome, mt-tRNA in specific, are the important causal factor in PCOS. BioMed Central 2019-07-24 /pmc/articles/PMC6657173/ /pubmed/31340838 http://dx.doi.org/10.1186/s13104-019-4453-3 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Note Saeed, Noor AlHuda Ali A. H. Hamzah, Israa Hussein Al-Gharrawi, Samar Abdul Raheem Polycystic ovary syndrome dependency on mtDNA mutation; copy Number and its association with insulin resistance |
title | Polycystic ovary syndrome dependency on mtDNA mutation; copy Number and its association with insulin resistance |
title_full | Polycystic ovary syndrome dependency on mtDNA mutation; copy Number and its association with insulin resistance |
title_fullStr | Polycystic ovary syndrome dependency on mtDNA mutation; copy Number and its association with insulin resistance |
title_full_unstemmed | Polycystic ovary syndrome dependency on mtDNA mutation; copy Number and its association with insulin resistance |
title_short | Polycystic ovary syndrome dependency on mtDNA mutation; copy Number and its association with insulin resistance |
title_sort | polycystic ovary syndrome dependency on mtdna mutation; copy number and its association with insulin resistance |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6657173/ https://www.ncbi.nlm.nih.gov/pubmed/31340838 http://dx.doi.org/10.1186/s13104-019-4453-3 |
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