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Etanercept protects ovarian reserve against ischemia/reperfusion injury in a rat model
INTRODUCTION: Etanercept has been widely used in autoimmune diseases for blocking tumor necrosis factor α (TNF-α), which is an inflammatory cytokine. The anti-apoptotic and anti-inflammatory effects of etanercept against ischemia/reperfusion (I/R) injury have been shown for several tissues in rat st...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6657239/ https://www.ncbi.nlm.nih.gov/pubmed/31360205 http://dx.doi.org/10.5114/aoms.2017.72406 |
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author | Eken, Meryem Kurek Ersoy, Gulcin Sahin Kaygusuz, Ecmel Işık Devranoğlu, Belgin Takır, Mümtaz Çilingir, Özlem Tuğçe Çevik, Özge |
author_facet | Eken, Meryem Kurek Ersoy, Gulcin Sahin Kaygusuz, Ecmel Işık Devranoğlu, Belgin Takır, Mümtaz Çilingir, Özlem Tuğçe Çevik, Özge |
author_sort | Eken, Meryem Kurek |
collection | PubMed |
description | INTRODUCTION: Etanercept has been widely used in autoimmune diseases for blocking tumor necrosis factor α (TNF-α), which is an inflammatory cytokine. The anti-apoptotic and anti-inflammatory effects of etanercept against ischemia/reperfusion (I/R) injury have been shown for several tissues in rat studies, but to the best of our knowledge, there are no reports on its protective effects following similar injury in ovarian tissue. The aim of this study was to investigate whether etanercept has beneficial effects on ovarian I/R injury, as well as on ovarian reserve. MATERIAL AND METHODS: Twenty-four rats were randomly divided into four groups (n = 6/group): sham (laparotomy only); sham + etanercept; I/R; and I/R + etanercept. Ischemia was induced for 3 h by twisting the ovary, and 24 h after detorsion the ovarian tissues were collected to evaluate histopathologic changes, glutathione (GSH), malondialdehyde (MDA), myeloperoxidase (MPO), and superoxide dismutase (SOD) concentrations for oxidative stress, 8-hydroxy-2′-deoxyguanosine (8-OHdG) for DNA damage, caspase-3 activity for apoptosis and ovarian follicle counts. To measure anti-Mullerian hormone (AMH), serum samples were drawn before and after surgery. RESULTS: Tissue GSH and SOD levels were significantly higher, while MDA and MPO levels were significantly lower in the I/R + etanercept group than in the I/R group (p < 0.05, p < 0.01, respectively). Tissue 8-OHdG and caspase-3 activity were significantly lower in the I/R+etanercept group than in the I/R group (p < 0.05, p < 0.01, respectively). Preoperative and postoperative AMH levels were compared and there was a significant reduction in the I/R and I/R + etanercept groups (p < 0.001, p < 0.001). The reduction of AMH in the I/R + etanercept group was significantly lower than in the I/R group. The primordial, preantral and small antral follicle numbers were also significantly higher in the I/R + etanercept group compared to the I/R group (p < 0.001, p < 0.001, p < 0.005, respectively). CONCLUSIONS: Etanercept attenuated inflammation and related oxidative stress and also helped to preserve ovarian reserve following ovarian I/R damage. |
format | Online Article Text |
id | pubmed-6657239 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-66572392019-07-29 Etanercept protects ovarian reserve against ischemia/reperfusion injury in a rat model Eken, Meryem Kurek Ersoy, Gulcin Sahin Kaygusuz, Ecmel Işık Devranoğlu, Belgin Takır, Mümtaz Çilingir, Özlem Tuğçe Çevik, Özge Arch Med Sci Experimental Research INTRODUCTION: Etanercept has been widely used in autoimmune diseases for blocking tumor necrosis factor α (TNF-α), which is an inflammatory cytokine. The anti-apoptotic and anti-inflammatory effects of etanercept against ischemia/reperfusion (I/R) injury have been shown for several tissues in rat studies, but to the best of our knowledge, there are no reports on its protective effects following similar injury in ovarian tissue. The aim of this study was to investigate whether etanercept has beneficial effects on ovarian I/R injury, as well as on ovarian reserve. MATERIAL AND METHODS: Twenty-four rats were randomly divided into four groups (n = 6/group): sham (laparotomy only); sham + etanercept; I/R; and I/R + etanercept. Ischemia was induced for 3 h by twisting the ovary, and 24 h after detorsion the ovarian tissues were collected to evaluate histopathologic changes, glutathione (GSH), malondialdehyde (MDA), myeloperoxidase (MPO), and superoxide dismutase (SOD) concentrations for oxidative stress, 8-hydroxy-2′-deoxyguanosine (8-OHdG) for DNA damage, caspase-3 activity for apoptosis and ovarian follicle counts. To measure anti-Mullerian hormone (AMH), serum samples were drawn before and after surgery. RESULTS: Tissue GSH and SOD levels were significantly higher, while MDA and MPO levels were significantly lower in the I/R + etanercept group than in the I/R group (p < 0.05, p < 0.01, respectively). Tissue 8-OHdG and caspase-3 activity were significantly lower in the I/R+etanercept group than in the I/R group (p < 0.05, p < 0.01, respectively). Preoperative and postoperative AMH levels were compared and there was a significant reduction in the I/R and I/R + etanercept groups (p < 0.001, p < 0.001). The reduction of AMH in the I/R + etanercept group was significantly lower than in the I/R group. The primordial, preantral and small antral follicle numbers were also significantly higher in the I/R + etanercept group compared to the I/R group (p < 0.001, p < 0.001, p < 0.005, respectively). CONCLUSIONS: Etanercept attenuated inflammation and related oxidative stress and also helped to preserve ovarian reserve following ovarian I/R damage. Termedia Publishing House 2019-02-25 2019-07 /pmc/articles/PMC6657239/ /pubmed/31360205 http://dx.doi.org/10.5114/aoms.2017.72406 Text en Copyright: © 2019 Termedia & Banach http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license. |
spellingShingle | Experimental Research Eken, Meryem Kurek Ersoy, Gulcin Sahin Kaygusuz, Ecmel Işık Devranoğlu, Belgin Takır, Mümtaz Çilingir, Özlem Tuğçe Çevik, Özge Etanercept protects ovarian reserve against ischemia/reperfusion injury in a rat model |
title | Etanercept protects ovarian reserve against ischemia/reperfusion injury in a rat model |
title_full | Etanercept protects ovarian reserve against ischemia/reperfusion injury in a rat model |
title_fullStr | Etanercept protects ovarian reserve against ischemia/reperfusion injury in a rat model |
title_full_unstemmed | Etanercept protects ovarian reserve against ischemia/reperfusion injury in a rat model |
title_short | Etanercept protects ovarian reserve against ischemia/reperfusion injury in a rat model |
title_sort | etanercept protects ovarian reserve against ischemia/reperfusion injury in a rat model |
topic | Experimental Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6657239/ https://www.ncbi.nlm.nih.gov/pubmed/31360205 http://dx.doi.org/10.5114/aoms.2017.72406 |
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