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Sasa Quelpaertensis Nakai Induced Antidepressant-Like Effect in Ovariectomized Rats

BACKGROUND: Sasa quelpaertensis Nakai extract (SQE) or dwarf bamboo has been extensively investigated for its antioxidant and anti-inflammatory effects; however, no previous study assessed its effect as an antidepressant agent. Therefore, this study was designed to examine the effect of oral SQE adm...

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Autores principales: Shaif, Noof Abdullah, Cho, Donghyun, Jang, Daehyuk, Kim, Hyung Min, Chung, Jin-Oh, Kim, Sunmi, Seo, Dae Bang, Kim, Kyu-Ri, Shin, Jaekyoon, Shim, Insop
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6657632/
https://www.ncbi.nlm.nih.gov/pubmed/31380432
http://dx.doi.org/10.1155/2019/5815604
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author Shaif, Noof Abdullah
Cho, Donghyun
Jang, Daehyuk
Kim, Hyung Min
Chung, Jin-Oh
Kim, Sunmi
Seo, Dae Bang
Kim, Kyu-Ri
Shin, Jaekyoon
Shim, Insop
author_facet Shaif, Noof Abdullah
Cho, Donghyun
Jang, Daehyuk
Kim, Hyung Min
Chung, Jin-Oh
Kim, Sunmi
Seo, Dae Bang
Kim, Kyu-Ri
Shin, Jaekyoon
Shim, Insop
author_sort Shaif, Noof Abdullah
collection PubMed
description BACKGROUND: Sasa quelpaertensis Nakai extract (SQE) or dwarf bamboo has been extensively investigated for its antioxidant and anti-inflammatory effects; however, no previous study assessed its effect as an antidepressant agent. Therefore, this study was designed to examine the effect of oral SQE administration in ameliorating menopausal depressive symptoms and to evaluate its mechanisms in ovariectomized rats with repeated stress. METHODS: All experimental groups except normal group underwent ovariectomy and then immobilization for 14 consecutive days. During these 2 weeks, two rat groups received SQE (100 and 300 mg/kg orally) and their cutaneous body temperature was measured. The tail suspension test (TST) and forced swim test (FST) were performed in order to evaluate depression-like behavior. Additionally, enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry were carried out to evaluate the central monoaminergic neurotransmitter levels and activity. RESULTS: Oral SQE (100 mg/kg) administration had reduced immobility time in TST and FST. Additionally, the SQE 100 and 300 mg/kg administration had decreased the cutaneous body temperature in the rats compared to those without treatment. In ELISA analysis, the SQE 100 group expressed elevated levels of serotonin and dopamine in the hypothalamus, prefrontal cortex, and hippocampus. Antityrosine hydroxylase (anti-TH) antibodies showed a tremendous increase in the density of TH positive cells in the locus coeruleus (LC) region of the SQE 100 group. Likewise, the SQE 100 elevated the number of tryptophan hydroxylase (TPH) and protein kinase C (PKC) immunoreactive cell counts and density in the hypothalamic region. CONCLUSION: These results suggested that the oral SQE administration induced the antidepressant-like effect in the ovariectomized rats with repeated stress via upregulating the levels of serotonin and dopamine through enhancing the expression of TH, TPH, and PKC in many brain areas.
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spelling pubmed-66576322019-08-04 Sasa Quelpaertensis Nakai Induced Antidepressant-Like Effect in Ovariectomized Rats Shaif, Noof Abdullah Cho, Donghyun Jang, Daehyuk Kim, Hyung Min Chung, Jin-Oh Kim, Sunmi Seo, Dae Bang Kim, Kyu-Ri Shin, Jaekyoon Shim, Insop Biomed Res Int Research Article BACKGROUND: Sasa quelpaertensis Nakai extract (SQE) or dwarf bamboo has been extensively investigated for its antioxidant and anti-inflammatory effects; however, no previous study assessed its effect as an antidepressant agent. Therefore, this study was designed to examine the effect of oral SQE administration in ameliorating menopausal depressive symptoms and to evaluate its mechanisms in ovariectomized rats with repeated stress. METHODS: All experimental groups except normal group underwent ovariectomy and then immobilization for 14 consecutive days. During these 2 weeks, two rat groups received SQE (100 and 300 mg/kg orally) and their cutaneous body temperature was measured. The tail suspension test (TST) and forced swim test (FST) were performed in order to evaluate depression-like behavior. Additionally, enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry were carried out to evaluate the central monoaminergic neurotransmitter levels and activity. RESULTS: Oral SQE (100 mg/kg) administration had reduced immobility time in TST and FST. Additionally, the SQE 100 and 300 mg/kg administration had decreased the cutaneous body temperature in the rats compared to those without treatment. In ELISA analysis, the SQE 100 group expressed elevated levels of serotonin and dopamine in the hypothalamus, prefrontal cortex, and hippocampus. Antityrosine hydroxylase (anti-TH) antibodies showed a tremendous increase in the density of TH positive cells in the locus coeruleus (LC) region of the SQE 100 group. Likewise, the SQE 100 elevated the number of tryptophan hydroxylase (TPH) and protein kinase C (PKC) immunoreactive cell counts and density in the hypothalamic region. CONCLUSION: These results suggested that the oral SQE administration induced the antidepressant-like effect in the ovariectomized rats with repeated stress via upregulating the levels of serotonin and dopamine through enhancing the expression of TH, TPH, and PKC in many brain areas. Hindawi 2019-07-11 /pmc/articles/PMC6657632/ /pubmed/31380432 http://dx.doi.org/10.1155/2019/5815604 Text en Copyright © 2019 Noof Abdullah Shaif et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shaif, Noof Abdullah
Cho, Donghyun
Jang, Daehyuk
Kim, Hyung Min
Chung, Jin-Oh
Kim, Sunmi
Seo, Dae Bang
Kim, Kyu-Ri
Shin, Jaekyoon
Shim, Insop
Sasa Quelpaertensis Nakai Induced Antidepressant-Like Effect in Ovariectomized Rats
title Sasa Quelpaertensis Nakai Induced Antidepressant-Like Effect in Ovariectomized Rats
title_full Sasa Quelpaertensis Nakai Induced Antidepressant-Like Effect in Ovariectomized Rats
title_fullStr Sasa Quelpaertensis Nakai Induced Antidepressant-Like Effect in Ovariectomized Rats
title_full_unstemmed Sasa Quelpaertensis Nakai Induced Antidepressant-Like Effect in Ovariectomized Rats
title_short Sasa Quelpaertensis Nakai Induced Antidepressant-Like Effect in Ovariectomized Rats
title_sort sasa quelpaertensis nakai induced antidepressant-like effect in ovariectomized rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6657632/
https://www.ncbi.nlm.nih.gov/pubmed/31380432
http://dx.doi.org/10.1155/2019/5815604
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