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Hybrid Nanofiber Scaffold-Based Direct Conversion of Neural Precursor Cells/Dopamine Neurons
The concept of cellular reprogramming was developed to generate induced neural precursor cells (iNPCs)/dopaminergic (iDA) neurons using diverse approaches. Here, we investigated the effects of various nanoscale scaffolds (fiber, dot, and line) on iNPC/iDA differentiation by direct reprogramming. The...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Stem Cell Research
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6657951/ https://www.ncbi.nlm.nih.gov/pubmed/31023000 http://dx.doi.org/10.15283/ijsc18123 |
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author | Lim, Mi-Sun Ko, Seung Hwan Kim, Min Sung Lee, Byungjun Jung, Ho-Sup Kim, Keesung Park, Chang-Hwan |
author_facet | Lim, Mi-Sun Ko, Seung Hwan Kim, Min Sung Lee, Byungjun Jung, Ho-Sup Kim, Keesung Park, Chang-Hwan |
author_sort | Lim, Mi-Sun |
collection | PubMed |
description | The concept of cellular reprogramming was developed to generate induced neural precursor cells (iNPCs)/dopaminergic (iDA) neurons using diverse approaches. Here, we investigated the effects of various nanoscale scaffolds (fiber, dot, and line) on iNPC/iDA differentiation by direct reprogramming. The generation and maturation of iDA neurons (microtubule-associated protein 2-positive and tyrosine hydroxylase-positive) and iNPCs (NESTIN-positive and SOX2-positive) increased on fiber and dot scaffolds as compared to that of the flat (control) scaffold. This study demonstrates that nanotopographical environments are suitable for direct differentiation methods and may improve the differentiation efficiency. |
format | Online Article Text |
id | pubmed-6657951 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Korean Society for Stem Cell Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-66579512019-07-29 Hybrid Nanofiber Scaffold-Based Direct Conversion of Neural Precursor Cells/Dopamine Neurons Lim, Mi-Sun Ko, Seung Hwan Kim, Min Sung Lee, Byungjun Jung, Ho-Sup Kim, Keesung Park, Chang-Hwan Int J Stem Cells Original Article The concept of cellular reprogramming was developed to generate induced neural precursor cells (iNPCs)/dopaminergic (iDA) neurons using diverse approaches. Here, we investigated the effects of various nanoscale scaffolds (fiber, dot, and line) on iNPC/iDA differentiation by direct reprogramming. The generation and maturation of iDA neurons (microtubule-associated protein 2-positive and tyrosine hydroxylase-positive) and iNPCs (NESTIN-positive and SOX2-positive) increased on fiber and dot scaffolds as compared to that of the flat (control) scaffold. This study demonstrates that nanotopographical environments are suitable for direct differentiation methods and may improve the differentiation efficiency. Korean Society for Stem Cell Research 2019-04-30 /pmc/articles/PMC6657951/ /pubmed/31023000 http://dx.doi.org/10.15283/ijsc18123 Text en Copyright © 2019 by the Korean Society for Stem Cell Research This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Lim, Mi-Sun Ko, Seung Hwan Kim, Min Sung Lee, Byungjun Jung, Ho-Sup Kim, Keesung Park, Chang-Hwan Hybrid Nanofiber Scaffold-Based Direct Conversion of Neural Precursor Cells/Dopamine Neurons |
title | Hybrid Nanofiber Scaffold-Based Direct Conversion of Neural Precursor Cells/Dopamine Neurons |
title_full | Hybrid Nanofiber Scaffold-Based Direct Conversion of Neural Precursor Cells/Dopamine Neurons |
title_fullStr | Hybrid Nanofiber Scaffold-Based Direct Conversion of Neural Precursor Cells/Dopamine Neurons |
title_full_unstemmed | Hybrid Nanofiber Scaffold-Based Direct Conversion of Neural Precursor Cells/Dopamine Neurons |
title_short | Hybrid Nanofiber Scaffold-Based Direct Conversion of Neural Precursor Cells/Dopamine Neurons |
title_sort | hybrid nanofiber scaffold-based direct conversion of neural precursor cells/dopamine neurons |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6657951/ https://www.ncbi.nlm.nih.gov/pubmed/31023000 http://dx.doi.org/10.15283/ijsc18123 |
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