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The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects

Although miRNAs have been implicated in the osteogenic differentiation of stem cells, their role in bone repair and reconstruction in tissue-engineered bone grafts remains unclear. We previously reported that microRNA (miR)-26a-5p inhibited the osteogenic differentiation of adipose-derived mesenchym...

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Autores principales: Yuan, Xiaoyan, Han, Lu, Lin, Hai, Guo, Zeyou, Huang, Yanling, Li, Shasha, Long, Ting, Tang, Wei, Tian, Weidong, Long, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658005/
https://www.ncbi.nlm.nih.gov/pubmed/31257525
http://dx.doi.org/10.3892/ijmm.2019.4249
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author Yuan, Xiaoyan
Han, Lu
Lin, Hai
Guo, Zeyou
Huang, Yanling
Li, Shasha
Long, Ting
Tang, Wei
Tian, Weidong
Long, Jie
author_facet Yuan, Xiaoyan
Han, Lu
Lin, Hai
Guo, Zeyou
Huang, Yanling
Li, Shasha
Long, Ting
Tang, Wei
Tian, Weidong
Long, Jie
author_sort Yuan, Xiaoyan
collection PubMed
description Although miRNAs have been implicated in the osteogenic differentiation of stem cells, their role in bone repair and reconstruction in tissue-engineered bone grafts remains unclear. We previously reported that microRNA (miR)-26a-5p inhibited the osteogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs), and that antimiR-26a-5p exerted the opposite effect. In the present study, the role of miR-26a-5p- and antimiR-26a-5p-modified ADSCs combined with biphasic calcium phosphate (BCP) scaffolds was evaluated in a rat femur defect model. The aim of the present study was to improve the understanding of the role of miR-26a-5p in bone regeneration in vivo, as well as to provide a new method to optimize the osteogenic ability of BCPs. ADSCs were infected with Lv-miR-26a-5p, Lv-miR-NC, Lv-antimiR-26a-5p or Lv-antimiR-NC respectively, and then combined with BCP scaffolds to repair rat femoral defects. Using X-rays, micro-computed tomography and histology at 2, 4, and 8 weeks postoperatively, the quantity and rate of bone regeneration were analyzed, revealing that they were the highest in animals treated with antimiR-26a-5p and the lowest in the miR-26a-5p treatment group. The expression levels of osteocalcin, collagen I, Runt-related transcription factor 2, Wnt family member 5A and calmodulin-dependent protein kinase II proteins were positively correlated with the bone formation rate. Taken together, the present results demonstrated that miR-26a-5p inhibited bone formation while antimiR-26a-5p accelerated bone formation via the Wnt/Ca(2+) signaling pathway. Therefore, antimiR-26a-5p-modified ADSCs combined with BCP scaffolds may be used to construct an effective tissue-engineering bone graft for bone repair and reconstruction.
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spelling pubmed-66580052019-08-07 The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects Yuan, Xiaoyan Han, Lu Lin, Hai Guo, Zeyou Huang, Yanling Li, Shasha Long, Ting Tang, Wei Tian, Weidong Long, Jie Int J Mol Med Articles Although miRNAs have been implicated in the osteogenic differentiation of stem cells, their role in bone repair and reconstruction in tissue-engineered bone grafts remains unclear. We previously reported that microRNA (miR)-26a-5p inhibited the osteogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs), and that antimiR-26a-5p exerted the opposite effect. In the present study, the role of miR-26a-5p- and antimiR-26a-5p-modified ADSCs combined with biphasic calcium phosphate (BCP) scaffolds was evaluated in a rat femur defect model. The aim of the present study was to improve the understanding of the role of miR-26a-5p in bone regeneration in vivo, as well as to provide a new method to optimize the osteogenic ability of BCPs. ADSCs were infected with Lv-miR-26a-5p, Lv-miR-NC, Lv-antimiR-26a-5p or Lv-antimiR-NC respectively, and then combined with BCP scaffolds to repair rat femoral defects. Using X-rays, micro-computed tomography and histology at 2, 4, and 8 weeks postoperatively, the quantity and rate of bone regeneration were analyzed, revealing that they were the highest in animals treated with antimiR-26a-5p and the lowest in the miR-26a-5p treatment group. The expression levels of osteocalcin, collagen I, Runt-related transcription factor 2, Wnt family member 5A and calmodulin-dependent protein kinase II proteins were positively correlated with the bone formation rate. Taken together, the present results demonstrated that miR-26a-5p inhibited bone formation while antimiR-26a-5p accelerated bone formation via the Wnt/Ca(2+) signaling pathway. Therefore, antimiR-26a-5p-modified ADSCs combined with BCP scaffolds may be used to construct an effective tissue-engineering bone graft for bone repair and reconstruction. D.A. Spandidos 2019-09 2019-06-20 /pmc/articles/PMC6658005/ /pubmed/31257525 http://dx.doi.org/10.3892/ijmm.2019.4249 Text en Copyright: © Yuan et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Yuan, Xiaoyan
Han, Lu
Lin, Hai
Guo, Zeyou
Huang, Yanling
Li, Shasha
Long, Ting
Tang, Wei
Tian, Weidong
Long, Jie
The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects
title The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects
title_full The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects
title_fullStr The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects
title_full_unstemmed The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects
title_short The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects
title_sort role of antimir-26a-5p/biphasic calcium phosphate in repairing rat femoral defects
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658005/
https://www.ncbi.nlm.nih.gov/pubmed/31257525
http://dx.doi.org/10.3892/ijmm.2019.4249
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