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The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects
Although miRNAs have been implicated in the osteogenic differentiation of stem cells, their role in bone repair and reconstruction in tissue-engineered bone grafts remains unclear. We previously reported that microRNA (miR)-26a-5p inhibited the osteogenic differentiation of adipose-derived mesenchym...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658005/ https://www.ncbi.nlm.nih.gov/pubmed/31257525 http://dx.doi.org/10.3892/ijmm.2019.4249 |
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author | Yuan, Xiaoyan Han, Lu Lin, Hai Guo, Zeyou Huang, Yanling Li, Shasha Long, Ting Tang, Wei Tian, Weidong Long, Jie |
author_facet | Yuan, Xiaoyan Han, Lu Lin, Hai Guo, Zeyou Huang, Yanling Li, Shasha Long, Ting Tang, Wei Tian, Weidong Long, Jie |
author_sort | Yuan, Xiaoyan |
collection | PubMed |
description | Although miRNAs have been implicated in the osteogenic differentiation of stem cells, their role in bone repair and reconstruction in tissue-engineered bone grafts remains unclear. We previously reported that microRNA (miR)-26a-5p inhibited the osteogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs), and that antimiR-26a-5p exerted the opposite effect. In the present study, the role of miR-26a-5p- and antimiR-26a-5p-modified ADSCs combined with biphasic calcium phosphate (BCP) scaffolds was evaluated in a rat femur defect model. The aim of the present study was to improve the understanding of the role of miR-26a-5p in bone regeneration in vivo, as well as to provide a new method to optimize the osteogenic ability of BCPs. ADSCs were infected with Lv-miR-26a-5p, Lv-miR-NC, Lv-antimiR-26a-5p or Lv-antimiR-NC respectively, and then combined with BCP scaffolds to repair rat femoral defects. Using X-rays, micro-computed tomography and histology at 2, 4, and 8 weeks postoperatively, the quantity and rate of bone regeneration were analyzed, revealing that they were the highest in animals treated with antimiR-26a-5p and the lowest in the miR-26a-5p treatment group. The expression levels of osteocalcin, collagen I, Runt-related transcription factor 2, Wnt family member 5A and calmodulin-dependent protein kinase II proteins were positively correlated with the bone formation rate. Taken together, the present results demonstrated that miR-26a-5p inhibited bone formation while antimiR-26a-5p accelerated bone formation via the Wnt/Ca(2+) signaling pathway. Therefore, antimiR-26a-5p-modified ADSCs combined with BCP scaffolds may be used to construct an effective tissue-engineering bone graft for bone repair and reconstruction. |
format | Online Article Text |
id | pubmed-6658005 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-66580052019-08-07 The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects Yuan, Xiaoyan Han, Lu Lin, Hai Guo, Zeyou Huang, Yanling Li, Shasha Long, Ting Tang, Wei Tian, Weidong Long, Jie Int J Mol Med Articles Although miRNAs have been implicated in the osteogenic differentiation of stem cells, their role in bone repair and reconstruction in tissue-engineered bone grafts remains unclear. We previously reported that microRNA (miR)-26a-5p inhibited the osteogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs), and that antimiR-26a-5p exerted the opposite effect. In the present study, the role of miR-26a-5p- and antimiR-26a-5p-modified ADSCs combined with biphasic calcium phosphate (BCP) scaffolds was evaluated in a rat femur defect model. The aim of the present study was to improve the understanding of the role of miR-26a-5p in bone regeneration in vivo, as well as to provide a new method to optimize the osteogenic ability of BCPs. ADSCs were infected with Lv-miR-26a-5p, Lv-miR-NC, Lv-antimiR-26a-5p or Lv-antimiR-NC respectively, and then combined with BCP scaffolds to repair rat femoral defects. Using X-rays, micro-computed tomography and histology at 2, 4, and 8 weeks postoperatively, the quantity and rate of bone regeneration were analyzed, revealing that they were the highest in animals treated with antimiR-26a-5p and the lowest in the miR-26a-5p treatment group. The expression levels of osteocalcin, collagen I, Runt-related transcription factor 2, Wnt family member 5A and calmodulin-dependent protein kinase II proteins were positively correlated with the bone formation rate. Taken together, the present results demonstrated that miR-26a-5p inhibited bone formation while antimiR-26a-5p accelerated bone formation via the Wnt/Ca(2+) signaling pathway. Therefore, antimiR-26a-5p-modified ADSCs combined with BCP scaffolds may be used to construct an effective tissue-engineering bone graft for bone repair and reconstruction. D.A. Spandidos 2019-09 2019-06-20 /pmc/articles/PMC6658005/ /pubmed/31257525 http://dx.doi.org/10.3892/ijmm.2019.4249 Text en Copyright: © Yuan et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Yuan, Xiaoyan Han, Lu Lin, Hai Guo, Zeyou Huang, Yanling Li, Shasha Long, Ting Tang, Wei Tian, Weidong Long, Jie The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects |
title | The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects |
title_full | The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects |
title_fullStr | The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects |
title_full_unstemmed | The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects |
title_short | The role of antimiR-26a-5p/biphasic calcium phosphate in repairing rat femoral defects |
title_sort | role of antimir-26a-5p/biphasic calcium phosphate in repairing rat femoral defects |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658005/ https://www.ncbi.nlm.nih.gov/pubmed/31257525 http://dx.doi.org/10.3892/ijmm.2019.4249 |
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