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Interleukin-1β promotes interleulin-6 expression via ERK1/2 signaling pathway in canine dermal fibroblasts

Interleukin-6 (IL-6) is a pleiotropic cytokine involved in the regulation of the immune response and inflammation. In this study, we investigated effect of the proinflammatory cytokine interleukin-1β (IL-1β) on IL-6 expression in canine dermal fibroblasts. IL-1β induced IL-6 mRNA expression and prot...

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Autores principales: Kitanaka, Nanako, Nakano, Rei, Sugiura, Kanae, Kitanaka, Taku, Namba, Shinichi, Konno, Tadayoshi, Nakayama, Tomohiro, Sugiya, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658082/
https://www.ncbi.nlm.nih.gov/pubmed/31344106
http://dx.doi.org/10.1371/journal.pone.0220262
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author Kitanaka, Nanako
Nakano, Rei
Sugiura, Kanae
Kitanaka, Taku
Namba, Shinichi
Konno, Tadayoshi
Nakayama, Tomohiro
Sugiya, Hiroshi
author_facet Kitanaka, Nanako
Nakano, Rei
Sugiura, Kanae
Kitanaka, Taku
Namba, Shinichi
Konno, Tadayoshi
Nakayama, Tomohiro
Sugiya, Hiroshi
author_sort Kitanaka, Nanako
collection PubMed
description Interleukin-6 (IL-6) is a pleiotropic cytokine involved in the regulation of the immune response and inflammation. In this study, we investigated effect of the proinflammatory cytokine interleukin-1β (IL-1β) on IL-6 expression in canine dermal fibroblasts. IL-1β induced IL-6 mRNA expression and protein release in a time- and dose-dependent manner. When cells were treated with inhibitors of mitogen-activated protein kinases (MAPKs), the extracellular signal-regulated kinase (ERK) inhibitor FR180240 inhibited IL-1β-induced IL-6 mRNA expression, but not SP600125 or SKF86002, which are c-Jun N-terminal kinase (JNK) and p38 MAPK inhibitors, respectively. In cells treated with U0126, an inhibitor of MAPK/ERK kinase (MEK), which activates ERK, IL-1β-induced IL-6 mRNA expression was also inhibited. IL-1β stimulated ERK1/2 phosphorylation. In cells transfected with ERK1 and ERK2 isoform siRNAs, IL-1β-induced IL-6 mRNA expression was reduced. These observations suggest that IL-1β induces IL-6 expression via ERK1/2 signaling pathway in canine dermal fibroblasts.
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spelling pubmed-66580822019-08-07 Interleukin-1β promotes interleulin-6 expression via ERK1/2 signaling pathway in canine dermal fibroblasts Kitanaka, Nanako Nakano, Rei Sugiura, Kanae Kitanaka, Taku Namba, Shinichi Konno, Tadayoshi Nakayama, Tomohiro Sugiya, Hiroshi PLoS One Research Article Interleukin-6 (IL-6) is a pleiotropic cytokine involved in the regulation of the immune response and inflammation. In this study, we investigated effect of the proinflammatory cytokine interleukin-1β (IL-1β) on IL-6 expression in canine dermal fibroblasts. IL-1β induced IL-6 mRNA expression and protein release in a time- and dose-dependent manner. When cells were treated with inhibitors of mitogen-activated protein kinases (MAPKs), the extracellular signal-regulated kinase (ERK) inhibitor FR180240 inhibited IL-1β-induced IL-6 mRNA expression, but not SP600125 or SKF86002, which are c-Jun N-terminal kinase (JNK) and p38 MAPK inhibitors, respectively. In cells treated with U0126, an inhibitor of MAPK/ERK kinase (MEK), which activates ERK, IL-1β-induced IL-6 mRNA expression was also inhibited. IL-1β stimulated ERK1/2 phosphorylation. In cells transfected with ERK1 and ERK2 isoform siRNAs, IL-1β-induced IL-6 mRNA expression was reduced. These observations suggest that IL-1β induces IL-6 expression via ERK1/2 signaling pathway in canine dermal fibroblasts. Public Library of Science 2019-07-25 /pmc/articles/PMC6658082/ /pubmed/31344106 http://dx.doi.org/10.1371/journal.pone.0220262 Text en © 2019 Kitanaka et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kitanaka, Nanako
Nakano, Rei
Sugiura, Kanae
Kitanaka, Taku
Namba, Shinichi
Konno, Tadayoshi
Nakayama, Tomohiro
Sugiya, Hiroshi
Interleukin-1β promotes interleulin-6 expression via ERK1/2 signaling pathway in canine dermal fibroblasts
title Interleukin-1β promotes interleulin-6 expression via ERK1/2 signaling pathway in canine dermal fibroblasts
title_full Interleukin-1β promotes interleulin-6 expression via ERK1/2 signaling pathway in canine dermal fibroblasts
title_fullStr Interleukin-1β promotes interleulin-6 expression via ERK1/2 signaling pathway in canine dermal fibroblasts
title_full_unstemmed Interleukin-1β promotes interleulin-6 expression via ERK1/2 signaling pathway in canine dermal fibroblasts
title_short Interleukin-1β promotes interleulin-6 expression via ERK1/2 signaling pathway in canine dermal fibroblasts
title_sort interleukin-1β promotes interleulin-6 expression via erk1/2 signaling pathway in canine dermal fibroblasts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658082/
https://www.ncbi.nlm.nih.gov/pubmed/31344106
http://dx.doi.org/10.1371/journal.pone.0220262
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