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Prevalence and subtype distribution of Blastocystis in ethnic minority groups on both sides of the China–Myanmar border, and assessment of risk factors

Blastocystis is one of the most common intestinal parasites in humans worldwide. To understand its prevalence and to explore the risk factors for Blastocystis in humans in developing countries, a molecular epidemiological investigation of Blastocystis was conducted in ethnic minority groups on both...

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Autores principales: Gong, Baiyan, Liu, Xiaohua, Wu, Yanchen, Xu, Ning, Xu, Meng, Yang, Fengkun, Tong, Lei, Zhou, Kexin, Cao, Jianping, Liu, Aiqin, Shen, Yujuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658150/
https://www.ncbi.nlm.nih.gov/pubmed/31343971
http://dx.doi.org/10.1051/parasite/2019046
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author Gong, Baiyan
Liu, Xiaohua
Wu, Yanchen
Xu, Ning
Xu, Meng
Yang, Fengkun
Tong, Lei
Zhou, Kexin
Cao, Jianping
Liu, Aiqin
Shen, Yujuan
author_facet Gong, Baiyan
Liu, Xiaohua
Wu, Yanchen
Xu, Ning
Xu, Meng
Yang, Fengkun
Tong, Lei
Zhou, Kexin
Cao, Jianping
Liu, Aiqin
Shen, Yujuan
author_sort Gong, Baiyan
collection PubMed
description Blastocystis is one of the most common intestinal parasites in humans worldwide. To understand its prevalence and to explore the risk factors for Blastocystis in humans in developing countries, a molecular epidemiological investigation of Blastocystis was conducted in ethnic minority groups on both sides of the China–Myanmar border. A total of 461 fecal specimens were collected from 289 Yao people in China and 172 Wa people in Myanmar, together with a questionnaire for each participant. Based on sequence analysis of the partial small subunit of ribosomal RNA (SSU rRNA) gene (barcode region or 260 bp region), an average prevalence of 6.29% (29/461) was observed, with 4.50% (13/289) in Yao people and 9.30% (16/172) in Wa people. Twenty-two Blastocystis isolates were successfully subtyped by sequence analysis of the barcode region. Three subtypes were identified: ST1 (n = 7), ST3 (n = 13) and ST4 (n = 2). A statistical difference in the prevalence of Blastocystis was only observed between children (12.37%, 12/97) and adults (4.95%, 16/323), and between not washing hands (11.02%, 14/127) and washing hands (4.76%, 15/315) after using toilets, emphasizing the importance and necessity of health education in people in the investigated areas, especially in children.
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spelling pubmed-66581502019-08-07 Prevalence and subtype distribution of Blastocystis in ethnic minority groups on both sides of the China–Myanmar border, and assessment of risk factors Gong, Baiyan Liu, Xiaohua Wu, Yanchen Xu, Ning Xu, Meng Yang, Fengkun Tong, Lei Zhou, Kexin Cao, Jianping Liu, Aiqin Shen, Yujuan Parasite Research Article Blastocystis is one of the most common intestinal parasites in humans worldwide. To understand its prevalence and to explore the risk factors for Blastocystis in humans in developing countries, a molecular epidemiological investigation of Blastocystis was conducted in ethnic minority groups on both sides of the China–Myanmar border. A total of 461 fecal specimens were collected from 289 Yao people in China and 172 Wa people in Myanmar, together with a questionnaire for each participant. Based on sequence analysis of the partial small subunit of ribosomal RNA (SSU rRNA) gene (barcode region or 260 bp region), an average prevalence of 6.29% (29/461) was observed, with 4.50% (13/289) in Yao people and 9.30% (16/172) in Wa people. Twenty-two Blastocystis isolates were successfully subtyped by sequence analysis of the barcode region. Three subtypes were identified: ST1 (n = 7), ST3 (n = 13) and ST4 (n = 2). A statistical difference in the prevalence of Blastocystis was only observed between children (12.37%, 12/97) and adults (4.95%, 16/323), and between not washing hands (11.02%, 14/127) and washing hands (4.76%, 15/315) after using toilets, emphasizing the importance and necessity of health education in people in the investigated areas, especially in children. EDP Sciences 2019-07-25 /pmc/articles/PMC6658150/ /pubmed/31343971 http://dx.doi.org/10.1051/parasite/2019046 Text en © B. Gong et al., published by EDP Sciences, 2019 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gong, Baiyan
Liu, Xiaohua
Wu, Yanchen
Xu, Ning
Xu, Meng
Yang, Fengkun
Tong, Lei
Zhou, Kexin
Cao, Jianping
Liu, Aiqin
Shen, Yujuan
Prevalence and subtype distribution of Blastocystis in ethnic minority groups on both sides of the China–Myanmar border, and assessment of risk factors
title Prevalence and subtype distribution of Blastocystis in ethnic minority groups on both sides of the China–Myanmar border, and assessment of risk factors
title_full Prevalence and subtype distribution of Blastocystis in ethnic minority groups on both sides of the China–Myanmar border, and assessment of risk factors
title_fullStr Prevalence and subtype distribution of Blastocystis in ethnic minority groups on both sides of the China–Myanmar border, and assessment of risk factors
title_full_unstemmed Prevalence and subtype distribution of Blastocystis in ethnic minority groups on both sides of the China–Myanmar border, and assessment of risk factors
title_short Prevalence and subtype distribution of Blastocystis in ethnic minority groups on both sides of the China–Myanmar border, and assessment of risk factors
title_sort prevalence and subtype distribution of blastocystis in ethnic minority groups on both sides of the china–myanmar border, and assessment of risk factors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658150/
https://www.ncbi.nlm.nih.gov/pubmed/31343971
http://dx.doi.org/10.1051/parasite/2019046
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