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Prevalence and subtype distribution of Blastocystis in ethnic minority groups on both sides of the China–Myanmar border, and assessment of risk factors
Blastocystis is one of the most common intestinal parasites in humans worldwide. To understand its prevalence and to explore the risk factors for Blastocystis in humans in developing countries, a molecular epidemiological investigation of Blastocystis was conducted in ethnic minority groups on both...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
EDP Sciences
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658150/ https://www.ncbi.nlm.nih.gov/pubmed/31343971 http://dx.doi.org/10.1051/parasite/2019046 |
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author | Gong, Baiyan Liu, Xiaohua Wu, Yanchen Xu, Ning Xu, Meng Yang, Fengkun Tong, Lei Zhou, Kexin Cao, Jianping Liu, Aiqin Shen, Yujuan |
author_facet | Gong, Baiyan Liu, Xiaohua Wu, Yanchen Xu, Ning Xu, Meng Yang, Fengkun Tong, Lei Zhou, Kexin Cao, Jianping Liu, Aiqin Shen, Yujuan |
author_sort | Gong, Baiyan |
collection | PubMed |
description | Blastocystis is one of the most common intestinal parasites in humans worldwide. To understand its prevalence and to explore the risk factors for Blastocystis in humans in developing countries, a molecular epidemiological investigation of Blastocystis was conducted in ethnic minority groups on both sides of the China–Myanmar border. A total of 461 fecal specimens were collected from 289 Yao people in China and 172 Wa people in Myanmar, together with a questionnaire for each participant. Based on sequence analysis of the partial small subunit of ribosomal RNA (SSU rRNA) gene (barcode region or 260 bp region), an average prevalence of 6.29% (29/461) was observed, with 4.50% (13/289) in Yao people and 9.30% (16/172) in Wa people. Twenty-two Blastocystis isolates were successfully subtyped by sequence analysis of the barcode region. Three subtypes were identified: ST1 (n = 7), ST3 (n = 13) and ST4 (n = 2). A statistical difference in the prevalence of Blastocystis was only observed between children (12.37%, 12/97) and adults (4.95%, 16/323), and between not washing hands (11.02%, 14/127) and washing hands (4.76%, 15/315) after using toilets, emphasizing the importance and necessity of health education in people in the investigated areas, especially in children. |
format | Online Article Text |
id | pubmed-6658150 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | EDP Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-66581502019-08-07 Prevalence and subtype distribution of Blastocystis in ethnic minority groups on both sides of the China–Myanmar border, and assessment of risk factors Gong, Baiyan Liu, Xiaohua Wu, Yanchen Xu, Ning Xu, Meng Yang, Fengkun Tong, Lei Zhou, Kexin Cao, Jianping Liu, Aiqin Shen, Yujuan Parasite Research Article Blastocystis is one of the most common intestinal parasites in humans worldwide. To understand its prevalence and to explore the risk factors for Blastocystis in humans in developing countries, a molecular epidemiological investigation of Blastocystis was conducted in ethnic minority groups on both sides of the China–Myanmar border. A total of 461 fecal specimens were collected from 289 Yao people in China and 172 Wa people in Myanmar, together with a questionnaire for each participant. Based on sequence analysis of the partial small subunit of ribosomal RNA (SSU rRNA) gene (barcode region or 260 bp region), an average prevalence of 6.29% (29/461) was observed, with 4.50% (13/289) in Yao people and 9.30% (16/172) in Wa people. Twenty-two Blastocystis isolates were successfully subtyped by sequence analysis of the barcode region. Three subtypes were identified: ST1 (n = 7), ST3 (n = 13) and ST4 (n = 2). A statistical difference in the prevalence of Blastocystis was only observed between children (12.37%, 12/97) and adults (4.95%, 16/323), and between not washing hands (11.02%, 14/127) and washing hands (4.76%, 15/315) after using toilets, emphasizing the importance and necessity of health education in people in the investigated areas, especially in children. EDP Sciences 2019-07-25 /pmc/articles/PMC6658150/ /pubmed/31343971 http://dx.doi.org/10.1051/parasite/2019046 Text en © B. Gong et al., published by EDP Sciences, 2019 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Gong, Baiyan Liu, Xiaohua Wu, Yanchen Xu, Ning Xu, Meng Yang, Fengkun Tong, Lei Zhou, Kexin Cao, Jianping Liu, Aiqin Shen, Yujuan Prevalence and subtype distribution of Blastocystis in ethnic minority groups on both sides of the China–Myanmar border, and assessment of risk factors |
title | Prevalence and subtype distribution of Blastocystis in ethnic minority groups on both sides of the China–Myanmar border, and assessment of risk factors |
title_full | Prevalence and subtype distribution of Blastocystis in ethnic minority groups on both sides of the China–Myanmar border, and assessment of risk factors |
title_fullStr | Prevalence and subtype distribution of Blastocystis in ethnic minority groups on both sides of the China–Myanmar border, and assessment of risk factors |
title_full_unstemmed | Prevalence and subtype distribution of Blastocystis in ethnic minority groups on both sides of the China–Myanmar border, and assessment of risk factors |
title_short | Prevalence and subtype distribution of Blastocystis in ethnic minority groups on both sides of the China–Myanmar border, and assessment of risk factors |
title_sort | prevalence and subtype distribution of blastocystis in ethnic minority groups on both sides of the china–myanmar border, and assessment of risk factors |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658150/ https://www.ncbi.nlm.nih.gov/pubmed/31343971 http://dx.doi.org/10.1051/parasite/2019046 |
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