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Activated Leukocyte Cell Adhesion Molecule Modulates Th2 Immune Response in Atopic Dermatitis

PURPOSE: Activated leukocyte cell adhesion molecule (ALCAM), a member of the immunoglobulin superfamily, is highly expressed on dendritic cells. ALCAM and its receptor CD6 are co-stimulatory molecules in the immunological synapse; their interaction is required for T cell activation. While atopic der...

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Autores principales: Oh, Mi Seon, Hong, Jung Yeon, Kim, Mi Na, Kwak, Eun Ji, Kim, Soo Yeon, Kim, Eun Gyul, Lee, Kyung Eun, Kim, Yun Seon, Jee, Hye Mi, Kim, Seo Hyeong, Sol, In Suk, Park, Chang Ook, Kim, Kyung Won, Sohn, Myung Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Academy of Asthma, Allergy and Clinical Immunology; The Korean Academy of Pediatric Allergy and Respiratory Disease 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658408/
https://www.ncbi.nlm.nih.gov/pubmed/31332979
http://dx.doi.org/10.4168/aair.2019.11.5.677
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author Oh, Mi Seon
Hong, Jung Yeon
Kim, Mi Na
Kwak, Eun Ji
Kim, Soo Yeon
Kim, Eun Gyul
Lee, Kyung Eun
Kim, Yun Seon
Jee, Hye Mi
Kim, Seo Hyeong
Sol, In Suk
Park, Chang Ook
Kim, Kyung Won
Sohn, Myung Hyun
author_facet Oh, Mi Seon
Hong, Jung Yeon
Kim, Mi Na
Kwak, Eun Ji
Kim, Soo Yeon
Kim, Eun Gyul
Lee, Kyung Eun
Kim, Yun Seon
Jee, Hye Mi
Kim, Seo Hyeong
Sol, In Suk
Park, Chang Ook
Kim, Kyung Won
Sohn, Myung Hyun
author_sort Oh, Mi Seon
collection PubMed
description PURPOSE: Activated leukocyte cell adhesion molecule (ALCAM), a member of the immunoglobulin superfamily, is highly expressed on dendritic cells. ALCAM and its receptor CD6 are co-stimulatory molecules in the immunological synapse; their interaction is required for T cell activation. While atopic dermatitis (AD) is recognized as a T helper 2 (Th2)-mediated allergic disease, the role of ALCAM in its pathogenesis is unclear. METHODS: ALCAM levels were measured in the serum of AD patients and AD-induced murine model by ovalbumin treatment. We next investigated transepidermal water loss, clinical score, Th2-immune responses, skin barrier gene expression and T-cell activation using wild-type (WT) and ALCAM deficiency mice. An oxazolone-induced AD-like model was also established and analyzed using WT- and ALCAM-deficient mice. RESULTS: We found that serum ALCAM levels were elevated in pediatric AD patients as well as WT AD mice, whereas Th2-type cytokine production and AD symptoms were suppressed in ALCAM-deficient mice. In addition, CD4(+) effector T-cell counts in murine skin and skin-draining lymph nodes were lower in ALCAM-deficient mice than in their WT counterparts. ALCAM deficiency was also linked to higher expression of skin barrier genes and number of lamellar bodies. CONCLUSIONS: These findings indicate that ALCAM may contribute to AD pathogenesis by meditating a Th2-dominant immune response and disrupting the barrier function of the skin.
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spelling pubmed-66584082019-09-01 Activated Leukocyte Cell Adhesion Molecule Modulates Th2 Immune Response in Atopic Dermatitis Oh, Mi Seon Hong, Jung Yeon Kim, Mi Na Kwak, Eun Ji Kim, Soo Yeon Kim, Eun Gyul Lee, Kyung Eun Kim, Yun Seon Jee, Hye Mi Kim, Seo Hyeong Sol, In Suk Park, Chang Ook Kim, Kyung Won Sohn, Myung Hyun Allergy Asthma Immunol Res Original Article PURPOSE: Activated leukocyte cell adhesion molecule (ALCAM), a member of the immunoglobulin superfamily, is highly expressed on dendritic cells. ALCAM and its receptor CD6 are co-stimulatory molecules in the immunological synapse; their interaction is required for T cell activation. While atopic dermatitis (AD) is recognized as a T helper 2 (Th2)-mediated allergic disease, the role of ALCAM in its pathogenesis is unclear. METHODS: ALCAM levels were measured in the serum of AD patients and AD-induced murine model by ovalbumin treatment. We next investigated transepidermal water loss, clinical score, Th2-immune responses, skin barrier gene expression and T-cell activation using wild-type (WT) and ALCAM deficiency mice. An oxazolone-induced AD-like model was also established and analyzed using WT- and ALCAM-deficient mice. RESULTS: We found that serum ALCAM levels were elevated in pediatric AD patients as well as WT AD mice, whereas Th2-type cytokine production and AD symptoms were suppressed in ALCAM-deficient mice. In addition, CD4(+) effector T-cell counts in murine skin and skin-draining lymph nodes were lower in ALCAM-deficient mice than in their WT counterparts. ALCAM deficiency was also linked to higher expression of skin barrier genes and number of lamellar bodies. CONCLUSIONS: These findings indicate that ALCAM may contribute to AD pathogenesis by meditating a Th2-dominant immune response and disrupting the barrier function of the skin. The Korean Academy of Asthma, Allergy and Clinical Immunology; The Korean Academy of Pediatric Allergy and Respiratory Disease 2019-06-03 /pmc/articles/PMC6658408/ /pubmed/31332979 http://dx.doi.org/10.4168/aair.2019.11.5.677 Text en Copyright © 2019 The Korean Academy of Asthma, Allergy and Clinical Immunology • The Korean Academy of Pediatric Allergy and Respiratory Disease https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Oh, Mi Seon
Hong, Jung Yeon
Kim, Mi Na
Kwak, Eun Ji
Kim, Soo Yeon
Kim, Eun Gyul
Lee, Kyung Eun
Kim, Yun Seon
Jee, Hye Mi
Kim, Seo Hyeong
Sol, In Suk
Park, Chang Ook
Kim, Kyung Won
Sohn, Myung Hyun
Activated Leukocyte Cell Adhesion Molecule Modulates Th2 Immune Response in Atopic Dermatitis
title Activated Leukocyte Cell Adhesion Molecule Modulates Th2 Immune Response in Atopic Dermatitis
title_full Activated Leukocyte Cell Adhesion Molecule Modulates Th2 Immune Response in Atopic Dermatitis
title_fullStr Activated Leukocyte Cell Adhesion Molecule Modulates Th2 Immune Response in Atopic Dermatitis
title_full_unstemmed Activated Leukocyte Cell Adhesion Molecule Modulates Th2 Immune Response in Atopic Dermatitis
title_short Activated Leukocyte Cell Adhesion Molecule Modulates Th2 Immune Response in Atopic Dermatitis
title_sort activated leukocyte cell adhesion molecule modulates th2 immune response in atopic dermatitis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658408/
https://www.ncbi.nlm.nih.gov/pubmed/31332979
http://dx.doi.org/10.4168/aair.2019.11.5.677
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