Cargando…
Otopathogenic Staphylococcus aureus Invades Human Middle Ear Epithelial Cells Primarily through Cholesterol Dependent Pathway
Chronic suppurative otitis media (CSOM) is one of the most common infectious diseases of the middle ear especially affecting children, leading to delay in language development and communication. Although Staphylococcus aureus is the most common pathogen associated with CSOM, its interaction with mid...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658548/ https://www.ncbi.nlm.nih.gov/pubmed/31346200 http://dx.doi.org/10.1038/s41598-019-47079-7 |
_version_ | 1783438978950627328 |
---|---|
author | Mittal, Rahul Debs, Luca H. Patel, Amit P. Nguyen, Desiree Blackwelder, Patricia Yan, Denise Weckwerth, Paulo H. Liu, Xue Zhong |
author_facet | Mittal, Rahul Debs, Luca H. Patel, Amit P. Nguyen, Desiree Blackwelder, Patricia Yan, Denise Weckwerth, Paulo H. Liu, Xue Zhong |
author_sort | Mittal, Rahul |
collection | PubMed |
description | Chronic suppurative otitis media (CSOM) is one of the most common infectious diseases of the middle ear especially affecting children, leading to delay in language development and communication. Although Staphylococcus aureus is the most common pathogen associated with CSOM, its interaction with middle ear epithelial cells is not well known. In the present study, we observed that otopathogenic S. aureus has the ability to invade human middle ear epithelial cells (HMEECs) in a dose and time dependent manner. Scanning electron microscopy demonstrated time dependent increase in the number of S. aureus on the surface of HMEECs. We observed that otopathogenic S. aureus primarily employs a cholesterol dependent pathway to colonize HMEECs. In agreement with these findings, confocal microscopy showed that S. aureus colocalized with lipid rafts in HMEECs. The results of the present study provide new insights into the pathogenesis of S. aureus induced CSOM. The availability of in vitro cell culture model will pave the way to develop novel effective treatment modalities for CSOM beyond antibiotic therapy. |
format | Online Article Text |
id | pubmed-6658548 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-66585482019-07-31 Otopathogenic Staphylococcus aureus Invades Human Middle Ear Epithelial Cells Primarily through Cholesterol Dependent Pathway Mittal, Rahul Debs, Luca H. Patel, Amit P. Nguyen, Desiree Blackwelder, Patricia Yan, Denise Weckwerth, Paulo H. Liu, Xue Zhong Sci Rep Article Chronic suppurative otitis media (CSOM) is one of the most common infectious diseases of the middle ear especially affecting children, leading to delay in language development and communication. Although Staphylococcus aureus is the most common pathogen associated with CSOM, its interaction with middle ear epithelial cells is not well known. In the present study, we observed that otopathogenic S. aureus has the ability to invade human middle ear epithelial cells (HMEECs) in a dose and time dependent manner. Scanning electron microscopy demonstrated time dependent increase in the number of S. aureus on the surface of HMEECs. We observed that otopathogenic S. aureus primarily employs a cholesterol dependent pathway to colonize HMEECs. In agreement with these findings, confocal microscopy showed that S. aureus colocalized with lipid rafts in HMEECs. The results of the present study provide new insights into the pathogenesis of S. aureus induced CSOM. The availability of in vitro cell culture model will pave the way to develop novel effective treatment modalities for CSOM beyond antibiotic therapy. Nature Publishing Group UK 2019-07-25 /pmc/articles/PMC6658548/ /pubmed/31346200 http://dx.doi.org/10.1038/s41598-019-47079-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Mittal, Rahul Debs, Luca H. Patel, Amit P. Nguyen, Desiree Blackwelder, Patricia Yan, Denise Weckwerth, Paulo H. Liu, Xue Zhong Otopathogenic Staphylococcus aureus Invades Human Middle Ear Epithelial Cells Primarily through Cholesterol Dependent Pathway |
title | Otopathogenic Staphylococcus aureus Invades Human Middle Ear Epithelial Cells Primarily through Cholesterol Dependent Pathway |
title_full | Otopathogenic Staphylococcus aureus Invades Human Middle Ear Epithelial Cells Primarily through Cholesterol Dependent Pathway |
title_fullStr | Otopathogenic Staphylococcus aureus Invades Human Middle Ear Epithelial Cells Primarily through Cholesterol Dependent Pathway |
title_full_unstemmed | Otopathogenic Staphylococcus aureus Invades Human Middle Ear Epithelial Cells Primarily through Cholesterol Dependent Pathway |
title_short | Otopathogenic Staphylococcus aureus Invades Human Middle Ear Epithelial Cells Primarily through Cholesterol Dependent Pathway |
title_sort | otopathogenic staphylococcus aureus invades human middle ear epithelial cells primarily through cholesterol dependent pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658548/ https://www.ncbi.nlm.nih.gov/pubmed/31346200 http://dx.doi.org/10.1038/s41598-019-47079-7 |
work_keys_str_mv | AT mittalrahul otopathogenicstaphylococcusaureusinvadeshumanmiddleearepithelialcellsprimarilythroughcholesteroldependentpathway AT debslucah otopathogenicstaphylococcusaureusinvadeshumanmiddleearepithelialcellsprimarilythroughcholesteroldependentpathway AT patelamitp otopathogenicstaphylococcusaureusinvadeshumanmiddleearepithelialcellsprimarilythroughcholesteroldependentpathway AT nguyendesiree otopathogenicstaphylococcusaureusinvadeshumanmiddleearepithelialcellsprimarilythroughcholesteroldependentpathway AT blackwelderpatricia otopathogenicstaphylococcusaureusinvadeshumanmiddleearepithelialcellsprimarilythroughcholesteroldependentpathway AT yandenise otopathogenicstaphylococcusaureusinvadeshumanmiddleearepithelialcellsprimarilythroughcholesteroldependentpathway AT weckwerthpauloh otopathogenicstaphylococcusaureusinvadeshumanmiddleearepithelialcellsprimarilythroughcholesteroldependentpathway AT liuxuezhong otopathogenicstaphylococcusaureusinvadeshumanmiddleearepithelialcellsprimarilythroughcholesteroldependentpathway |