A high-throughput screening method based on the Mizoroki-Heck reaction for isolating itaconic acid-producing fungi from soils

In this study, we report a novel method based on the Mizoroki-Heck reaction followed by an iodine test for the screening of itaconic acid-producing fungi from soils. This method is simple, rapid, and requires 10 μL of culture; results are obtained within 1.5 h. The detection limit of itaconic acid i...

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Autores principales: Sano, Mei, Kuroda, Hikari, Ohara, Hitomi, Ando, Hiroshi, Matsumoto, Keiji, Aso, Yuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658728/
https://www.ncbi.nlm.nih.gov/pubmed/31372531
http://dx.doi.org/10.1016/j.heliyon.2019.e02048
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author Sano, Mei
Kuroda, Hikari
Ohara, Hitomi
Ando, Hiroshi
Matsumoto, Keiji
Aso, Yuji
author_facet Sano, Mei
Kuroda, Hikari
Ohara, Hitomi
Ando, Hiroshi
Matsumoto, Keiji
Aso, Yuji
author_sort Sano, Mei
collection PubMed
description In this study, we report a novel method based on the Mizoroki-Heck reaction followed by an iodine test for the screening of itaconic acid-producing fungi from soils. This method is simple, rapid, and requires 10 μL of culture; results are obtained within 1.5 h. The detection limit of itaconic acid in the cultures was 0.13 mM. This is the first report on the direct screening of itaconic acid-producing fungi using a coupling reaction.
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spelling pubmed-66587282019-08-01 A high-throughput screening method based on the Mizoroki-Heck reaction for isolating itaconic acid-producing fungi from soils Sano, Mei Kuroda, Hikari Ohara, Hitomi Ando, Hiroshi Matsumoto, Keiji Aso, Yuji Heliyon Article In this study, we report a novel method based on the Mizoroki-Heck reaction followed by an iodine test for the screening of itaconic acid-producing fungi from soils. This method is simple, rapid, and requires 10 μL of culture; results are obtained within 1.5 h. The detection limit of itaconic acid in the cultures was 0.13 mM. This is the first report on the direct screening of itaconic acid-producing fungi using a coupling reaction. Elsevier 2019-07-22 /pmc/articles/PMC6658728/ /pubmed/31372531 http://dx.doi.org/10.1016/j.heliyon.2019.e02048 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Sano, Mei
Kuroda, Hikari
Ohara, Hitomi
Ando, Hiroshi
Matsumoto, Keiji
Aso, Yuji
A high-throughput screening method based on the Mizoroki-Heck reaction for isolating itaconic acid-producing fungi from soils
title A high-throughput screening method based on the Mizoroki-Heck reaction for isolating itaconic acid-producing fungi from soils
title_full A high-throughput screening method based on the Mizoroki-Heck reaction for isolating itaconic acid-producing fungi from soils
title_fullStr A high-throughput screening method based on the Mizoroki-Heck reaction for isolating itaconic acid-producing fungi from soils
title_full_unstemmed A high-throughput screening method based on the Mizoroki-Heck reaction for isolating itaconic acid-producing fungi from soils
title_short A high-throughput screening method based on the Mizoroki-Heck reaction for isolating itaconic acid-producing fungi from soils
title_sort high-throughput screening method based on the mizoroki-heck reaction for isolating itaconic acid-producing fungi from soils
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658728/
https://www.ncbi.nlm.nih.gov/pubmed/31372531
http://dx.doi.org/10.1016/j.heliyon.2019.e02048
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