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Novel differential linear B‐cell epitopes to identify Zika and dengue virus infections in patients

OBJECTIVES: Recent Zika virus (ZIKV) outbreaks challenged existing laboratory diagnostic standards, especially for serology‐based methods. Because of the genetic and structural similarity of ZIKV with other flaviviruses, this results in cross‐reactive antibodies, which confounds serological interpre...

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Autores principales: Amrun, Siti Naqiah, Yee, Wearn‐Xin, Abu Bakar, Farhana, Lee, Bernett, Kam, Yiu‐Wing, Lum, Fok‐Moon, Tan, Jeslin JL, Lim, Vanessa WX, Watthanaworawit, Wanitda, Ling, Clare, Nosten, Francois, Renia, Laurent, Leo, Yee‐Sin, Ng, Lisa FP
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6659153/
https://www.ncbi.nlm.nih.gov/pubmed/31372218
http://dx.doi.org/10.1002/cti2.1066
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author Amrun, Siti Naqiah
Yee, Wearn‐Xin
Abu Bakar, Farhana
Lee, Bernett
Kam, Yiu‐Wing
Lum, Fok‐Moon
Tan, Jeslin JL
Lim, Vanessa WX
Watthanaworawit, Wanitda
Ling, Clare
Nosten, Francois
Renia, Laurent
Leo, Yee‐Sin
Ng, Lisa FP
author_facet Amrun, Siti Naqiah
Yee, Wearn‐Xin
Abu Bakar, Farhana
Lee, Bernett
Kam, Yiu‐Wing
Lum, Fok‐Moon
Tan, Jeslin JL
Lim, Vanessa WX
Watthanaworawit, Wanitda
Ling, Clare
Nosten, Francois
Renia, Laurent
Leo, Yee‐Sin
Ng, Lisa FP
author_sort Amrun, Siti Naqiah
collection PubMed
description OBJECTIVES: Recent Zika virus (ZIKV) outbreaks challenged existing laboratory diagnostic standards, especially for serology‐based methods. Because of the genetic and structural similarity of ZIKV with other flaviviruses, this results in cross‐reactive antibodies, which confounds serological interpretations. METHODS: Plasma from Singapore ZIKV patients was screened longitudinally for antibody responses and neutralising capacities against ZIKV. Samples from healthy controls, ZIKV patients and DENV patients were further assessed using ZIKV and DENV peptides of precursor membrane (prM), envelope (E) or non‐structural 1 (NS1) viral proteins in a peptide‐based ELISA for epitope identification. Identified epitopes were re‐validated and diagnostically evaluated using sera of patients with DENV, bacteria or unknown infections from Thailand. RESULTS: Long‐lasting ZIKV‐neutralising antibodies were elicited during ZIKV infection. Thirteen potential linear B‐cell epitopes were identified, and of these, four common flavivirus, three ZIKV‐specific and one DENV‐specific differential epitopes had more than 50% sensitivity and specificity. Notably, ZIKV‐specific peptide 26 on domain I/II of E protein (amino acid residues 271–288) presented 80% sensitivity and 85.7% specificity. Importantly, the differential epitopes also showed significance in differentiating non‐flavivirus patient samples. CONCLUSION: Linear B‐cell epitope candidates to differentiate between ZIKV and DENV infections were identified, providing the first step towards the design of a much‐needed serology‐based assay.
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spelling pubmed-66591532019-08-01 Novel differential linear B‐cell epitopes to identify Zika and dengue virus infections in patients Amrun, Siti Naqiah Yee, Wearn‐Xin Abu Bakar, Farhana Lee, Bernett Kam, Yiu‐Wing Lum, Fok‐Moon Tan, Jeslin JL Lim, Vanessa WX Watthanaworawit, Wanitda Ling, Clare Nosten, Francois Renia, Laurent Leo, Yee‐Sin Ng, Lisa FP Clin Transl Immunology Original Articles OBJECTIVES: Recent Zika virus (ZIKV) outbreaks challenged existing laboratory diagnostic standards, especially for serology‐based methods. Because of the genetic and structural similarity of ZIKV with other flaviviruses, this results in cross‐reactive antibodies, which confounds serological interpretations. METHODS: Plasma from Singapore ZIKV patients was screened longitudinally for antibody responses and neutralising capacities against ZIKV. Samples from healthy controls, ZIKV patients and DENV patients were further assessed using ZIKV and DENV peptides of precursor membrane (prM), envelope (E) or non‐structural 1 (NS1) viral proteins in a peptide‐based ELISA for epitope identification. Identified epitopes were re‐validated and diagnostically evaluated using sera of patients with DENV, bacteria or unknown infections from Thailand. RESULTS: Long‐lasting ZIKV‐neutralising antibodies were elicited during ZIKV infection. Thirteen potential linear B‐cell epitopes were identified, and of these, four common flavivirus, three ZIKV‐specific and one DENV‐specific differential epitopes had more than 50% sensitivity and specificity. Notably, ZIKV‐specific peptide 26 on domain I/II of E protein (amino acid residues 271–288) presented 80% sensitivity and 85.7% specificity. Importantly, the differential epitopes also showed significance in differentiating non‐flavivirus patient samples. CONCLUSION: Linear B‐cell epitope candidates to differentiate between ZIKV and DENV infections were identified, providing the first step towards the design of a much‐needed serology‐based assay. John Wiley and Sons Inc. 2019-07-26 /pmc/articles/PMC6659153/ /pubmed/31372218 http://dx.doi.org/10.1002/cti2.1066 Text en © 2019 The Authors. Clinical & Translational Immunology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Amrun, Siti Naqiah
Yee, Wearn‐Xin
Abu Bakar, Farhana
Lee, Bernett
Kam, Yiu‐Wing
Lum, Fok‐Moon
Tan, Jeslin JL
Lim, Vanessa WX
Watthanaworawit, Wanitda
Ling, Clare
Nosten, Francois
Renia, Laurent
Leo, Yee‐Sin
Ng, Lisa FP
Novel differential linear B‐cell epitopes to identify Zika and dengue virus infections in patients
title Novel differential linear B‐cell epitopes to identify Zika and dengue virus infections in patients
title_full Novel differential linear B‐cell epitopes to identify Zika and dengue virus infections in patients
title_fullStr Novel differential linear B‐cell epitopes to identify Zika and dengue virus infections in patients
title_full_unstemmed Novel differential linear B‐cell epitopes to identify Zika and dengue virus infections in patients
title_short Novel differential linear B‐cell epitopes to identify Zika and dengue virus infections in patients
title_sort novel differential linear b‐cell epitopes to identify zika and dengue virus infections in patients
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6659153/
https://www.ncbi.nlm.nih.gov/pubmed/31372218
http://dx.doi.org/10.1002/cti2.1066
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