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Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO(2)
BACKGROUND: Increasing CO(2) emissions have resulted in ocean acidification, affecting marine plant photosynthesis and changing the nutrient composition of marine ecosystems. The physiological and biochemical processes of marine phytoplankton in response to ocean acidification have been reported, bu...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6659225/ https://www.ncbi.nlm.nih.gov/pubmed/31349823 http://dx.doi.org/10.1186/s12896-019-0544-4 |
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author | Huang, Aiyou Wu, Songcui Gu, Wenhui Li, Yuanxiang Xie, Xiujun Wang, Guangce |
author_facet | Huang, Aiyou Wu, Songcui Gu, Wenhui Li, Yuanxiang Xie, Xiujun Wang, Guangce |
author_sort | Huang, Aiyou |
collection | PubMed |
description | BACKGROUND: Increasing CO(2) emissions have resulted in ocean acidification, affecting marine plant photosynthesis and changing the nutrient composition of marine ecosystems. The physiological and biochemical processes of marine phytoplankton in response to ocean acidification have been reported, but have been mainly focused on growth and photosynthetic physiology. To acquire a thorough knowledge of the molecular regulation mechanisms, model species with clear genetic background should be selected for systematic study. Phaeodactylum tricornutum is a pennate diatom with the characteristics of small genome size, short generation cycle, and easy to transform. Furthermore, the genome of P. tricornutum has been completely sequenced. RESULTS AND DISCUSSION: In this study, P. tricornutum was cultured at high and normal CO(2) concentrations. Cell composition changes during culture time were investigated. The (13)C isotope tracing technique was used to determine fractional labeling enrichments for the main cellular components. The results suggested that when lipid content increased significantly under high CO(2) conditions, total protein and soluble sugar contents decreased. The (13)C labeling experiment indicated that the C skeleton needed for fatty acid C chain elongation in lipid synthesis under high CO(2) conditions is not mainly derived from NaHCO(3) (carbon fixed by photosynthesis). CONCLUSION: This study indicated that breakdown of intracellular protein and soluble sugar provide C skeleton for lipid synthesis under high CO(2) concentration. |
format | Online Article Text |
id | pubmed-6659225 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-66592252019-08-01 Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO(2) Huang, Aiyou Wu, Songcui Gu, Wenhui Li, Yuanxiang Xie, Xiujun Wang, Guangce BMC Biotechnol Research Article BACKGROUND: Increasing CO(2) emissions have resulted in ocean acidification, affecting marine plant photosynthesis and changing the nutrient composition of marine ecosystems. The physiological and biochemical processes of marine phytoplankton in response to ocean acidification have been reported, but have been mainly focused on growth and photosynthetic physiology. To acquire a thorough knowledge of the molecular regulation mechanisms, model species with clear genetic background should be selected for systematic study. Phaeodactylum tricornutum is a pennate diatom with the characteristics of small genome size, short generation cycle, and easy to transform. Furthermore, the genome of P. tricornutum has been completely sequenced. RESULTS AND DISCUSSION: In this study, P. tricornutum was cultured at high and normal CO(2) concentrations. Cell composition changes during culture time were investigated. The (13)C isotope tracing technique was used to determine fractional labeling enrichments for the main cellular components. The results suggested that when lipid content increased significantly under high CO(2) conditions, total protein and soluble sugar contents decreased. The (13)C labeling experiment indicated that the C skeleton needed for fatty acid C chain elongation in lipid synthesis under high CO(2) conditions is not mainly derived from NaHCO(3) (carbon fixed by photosynthesis). CONCLUSION: This study indicated that breakdown of intracellular protein and soluble sugar provide C skeleton for lipid synthesis under high CO(2) concentration. BioMed Central 2019-07-26 /pmc/articles/PMC6659225/ /pubmed/31349823 http://dx.doi.org/10.1186/s12896-019-0544-4 Text en © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Huang, Aiyou Wu, Songcui Gu, Wenhui Li, Yuanxiang Xie, Xiujun Wang, Guangce Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO(2) |
title | Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO(2) |
title_full | Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO(2) |
title_fullStr | Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO(2) |
title_full_unstemmed | Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO(2) |
title_short | Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO(2) |
title_sort | provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in phaeodactylum tricornutum under high co(2) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6659225/ https://www.ncbi.nlm.nih.gov/pubmed/31349823 http://dx.doi.org/10.1186/s12896-019-0544-4 |
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