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Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO(2)

BACKGROUND: Increasing CO(2) emissions have resulted in ocean acidification, affecting marine plant photosynthesis and changing the nutrient composition of marine ecosystems. The physiological and biochemical processes of marine phytoplankton in response to ocean acidification have been reported, bu...

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Autores principales: Huang, Aiyou, Wu, Songcui, Gu, Wenhui, Li, Yuanxiang, Xie, Xiujun, Wang, Guangce
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6659225/
https://www.ncbi.nlm.nih.gov/pubmed/31349823
http://dx.doi.org/10.1186/s12896-019-0544-4
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author Huang, Aiyou
Wu, Songcui
Gu, Wenhui
Li, Yuanxiang
Xie, Xiujun
Wang, Guangce
author_facet Huang, Aiyou
Wu, Songcui
Gu, Wenhui
Li, Yuanxiang
Xie, Xiujun
Wang, Guangce
author_sort Huang, Aiyou
collection PubMed
description BACKGROUND: Increasing CO(2) emissions have resulted in ocean acidification, affecting marine plant photosynthesis and changing the nutrient composition of marine ecosystems. The physiological and biochemical processes of marine phytoplankton in response to ocean acidification have been reported, but have been mainly focused on growth and photosynthetic physiology. To acquire a thorough knowledge of the molecular regulation mechanisms, model species with clear genetic background should be selected for systematic study. Phaeodactylum tricornutum is a pennate diatom with the characteristics of small genome size, short generation cycle, and easy to transform. Furthermore, the genome of P. tricornutum has been completely sequenced. RESULTS AND DISCUSSION: In this study, P. tricornutum was cultured at high and normal CO(2) concentrations. Cell composition changes during culture time were investigated. The (13)C isotope tracing technique was used to determine fractional labeling enrichments for the main cellular components. The results suggested that when lipid content increased significantly under high CO(2) conditions, total protein and soluble sugar contents decreased. The (13)C labeling experiment indicated that the C skeleton needed for fatty acid C chain elongation in lipid synthesis under high CO(2) conditions is not mainly derived from NaHCO(3) (carbon fixed by photosynthesis). CONCLUSION: This study indicated that breakdown of intracellular protein and soluble sugar provide C skeleton for lipid synthesis under high CO(2) concentration.
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spelling pubmed-66592252019-08-01 Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO(2) Huang, Aiyou Wu, Songcui Gu, Wenhui Li, Yuanxiang Xie, Xiujun Wang, Guangce BMC Biotechnol Research Article BACKGROUND: Increasing CO(2) emissions have resulted in ocean acidification, affecting marine plant photosynthesis and changing the nutrient composition of marine ecosystems. The physiological and biochemical processes of marine phytoplankton in response to ocean acidification have been reported, but have been mainly focused on growth and photosynthetic physiology. To acquire a thorough knowledge of the molecular regulation mechanisms, model species with clear genetic background should be selected for systematic study. Phaeodactylum tricornutum is a pennate diatom with the characteristics of small genome size, short generation cycle, and easy to transform. Furthermore, the genome of P. tricornutum has been completely sequenced. RESULTS AND DISCUSSION: In this study, P. tricornutum was cultured at high and normal CO(2) concentrations. Cell composition changes during culture time were investigated. The (13)C isotope tracing technique was used to determine fractional labeling enrichments for the main cellular components. The results suggested that when lipid content increased significantly under high CO(2) conditions, total protein and soluble sugar contents decreased. The (13)C labeling experiment indicated that the C skeleton needed for fatty acid C chain elongation in lipid synthesis under high CO(2) conditions is not mainly derived from NaHCO(3) (carbon fixed by photosynthesis). CONCLUSION: This study indicated that breakdown of intracellular protein and soluble sugar provide C skeleton for lipid synthesis under high CO(2) concentration. BioMed Central 2019-07-26 /pmc/articles/PMC6659225/ /pubmed/31349823 http://dx.doi.org/10.1186/s12896-019-0544-4 Text en © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Huang, Aiyou
Wu, Songcui
Gu, Wenhui
Li, Yuanxiang
Xie, Xiujun
Wang, Guangce
Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO(2)
title Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO(2)
title_full Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO(2)
title_fullStr Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO(2)
title_full_unstemmed Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO(2)
title_short Provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in Phaeodactylum tricornutum under high CO(2)
title_sort provision of carbon skeleton for lipid synthesis from the breakdown of intracellular protein and soluble sugar in phaeodactylum tricornutum under high co(2)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6659225/
https://www.ncbi.nlm.nih.gov/pubmed/31349823
http://dx.doi.org/10.1186/s12896-019-0544-4
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