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Enhanced morphological and functional differences of pancreatic cancer with epithelial or mesenchymal characteristics in 3D culture
Pancreatic cancer, composed of heterogeneous cancer cells, alters epithelial to mesenchymal features during growth and metastasis. In this study, we aimed to characterize pancreatic ductal adenocarcinoma (PDAC) cells showing epithelial or mesenchymal features in 3D culture. In 3D culture, PK-1 cells...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6659675/ https://www.ncbi.nlm.nih.gov/pubmed/31350453 http://dx.doi.org/10.1038/s41598-019-47416-w |
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author | Shichi, Yuuki Sasaki, Norihiko Michishita, Masaki Hasegawa, Fumio Matsuda, Yoko Arai, Tomio Gomi, Fujiya Aida, Junko Takubo, Kaiyo Toyoda, Masashi Yoshimura, Hisashi Takahashi, Kimimasa Ishiwata, Toshiyuki |
author_facet | Shichi, Yuuki Sasaki, Norihiko Michishita, Masaki Hasegawa, Fumio Matsuda, Yoko Arai, Tomio Gomi, Fujiya Aida, Junko Takubo, Kaiyo Toyoda, Masashi Yoshimura, Hisashi Takahashi, Kimimasa Ishiwata, Toshiyuki |
author_sort | Shichi, Yuuki |
collection | PubMed |
description | Pancreatic cancer, composed of heterogeneous cancer cells, alters epithelial to mesenchymal features during growth and metastasis. In this study, we aimed to characterize pancreatic ductal adenocarcinoma (PDAC) cells showing epithelial or mesenchymal features in 3D culture. In 3D culture, PK-1 cells had high E-cadherin and low vimentin expression and exhibited a round-like appearance encircled by flat cells. PANC-1 cells had high vimentin and low E-cadherin expression and formed grape-like spheres. PK-1 cells had secretary granules and many microvilli, desmosomes, and adherens junctions, while PANC-1 cells had few microvilli, adherens junction, and no desmosomes. Cytokeratin 7, trypsin, CA19-9, and E-cadherin were highly expressed in PK-1 cells but not in PANC-1 cells. Ki-67 was diffusely expressed in PANC-1 spheres but was restricted to the peripheral flat cells of PK-1 spheres. PANC-1 and PK-1 cells were positive for transforming growth factor (TGF) β receptor II and phosphorylated smad2/3, but PK-1 cells were smad4 negative. Taken together, 3D culture enhanced morphofunctional differences of PDAC cells showing epithelial or mesenchymal characteristics, and epithelial phenotype maintenance may be due to the ineffectiveness of the TGF- β pathway. Clarification of heterogeneity using 3D culture may be useful for development of individualized diagnostic and therapeutic methods in patients with PDAC. |
format | Online Article Text |
id | pubmed-6659675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-66596752019-08-01 Enhanced morphological and functional differences of pancreatic cancer with epithelial or mesenchymal characteristics in 3D culture Shichi, Yuuki Sasaki, Norihiko Michishita, Masaki Hasegawa, Fumio Matsuda, Yoko Arai, Tomio Gomi, Fujiya Aida, Junko Takubo, Kaiyo Toyoda, Masashi Yoshimura, Hisashi Takahashi, Kimimasa Ishiwata, Toshiyuki Sci Rep Article Pancreatic cancer, composed of heterogeneous cancer cells, alters epithelial to mesenchymal features during growth and metastasis. In this study, we aimed to characterize pancreatic ductal adenocarcinoma (PDAC) cells showing epithelial or mesenchymal features in 3D culture. In 3D culture, PK-1 cells had high E-cadherin and low vimentin expression and exhibited a round-like appearance encircled by flat cells. PANC-1 cells had high vimentin and low E-cadherin expression and formed grape-like spheres. PK-1 cells had secretary granules and many microvilli, desmosomes, and adherens junctions, while PANC-1 cells had few microvilli, adherens junction, and no desmosomes. Cytokeratin 7, trypsin, CA19-9, and E-cadherin were highly expressed in PK-1 cells but not in PANC-1 cells. Ki-67 was diffusely expressed in PANC-1 spheres but was restricted to the peripheral flat cells of PK-1 spheres. PANC-1 and PK-1 cells were positive for transforming growth factor (TGF) β receptor II and phosphorylated smad2/3, but PK-1 cells were smad4 negative. Taken together, 3D culture enhanced morphofunctional differences of PDAC cells showing epithelial or mesenchymal characteristics, and epithelial phenotype maintenance may be due to the ineffectiveness of the TGF- β pathway. Clarification of heterogeneity using 3D culture may be useful for development of individualized diagnostic and therapeutic methods in patients with PDAC. Nature Publishing Group UK 2019-07-26 /pmc/articles/PMC6659675/ /pubmed/31350453 http://dx.doi.org/10.1038/s41598-019-47416-w Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Shichi, Yuuki Sasaki, Norihiko Michishita, Masaki Hasegawa, Fumio Matsuda, Yoko Arai, Tomio Gomi, Fujiya Aida, Junko Takubo, Kaiyo Toyoda, Masashi Yoshimura, Hisashi Takahashi, Kimimasa Ishiwata, Toshiyuki Enhanced morphological and functional differences of pancreatic cancer with epithelial or mesenchymal characteristics in 3D culture |
title | Enhanced morphological and functional differences of pancreatic cancer with epithelial or mesenchymal characteristics in 3D culture |
title_full | Enhanced morphological and functional differences of pancreatic cancer with epithelial or mesenchymal characteristics in 3D culture |
title_fullStr | Enhanced morphological and functional differences of pancreatic cancer with epithelial or mesenchymal characteristics in 3D culture |
title_full_unstemmed | Enhanced morphological and functional differences of pancreatic cancer with epithelial or mesenchymal characteristics in 3D culture |
title_short | Enhanced morphological and functional differences of pancreatic cancer with epithelial or mesenchymal characteristics in 3D culture |
title_sort | enhanced morphological and functional differences of pancreatic cancer with epithelial or mesenchymal characteristics in 3d culture |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6659675/ https://www.ncbi.nlm.nih.gov/pubmed/31350453 http://dx.doi.org/10.1038/s41598-019-47416-w |
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