Cargando…
The Efficacy of 2% Topical Rebamipide on Conjunctival Squamous Metaplasia and Goblet Cell Density in Dry Eye Disease
Purpose: To clarify the pharmacological effects of 2% rebamipide eye drops on mucosal membrane functions of the ocular surface epithelium, we investigated keratoconjunctival alterations at the cellular level in this study. Methods: Fifteen patients with definite dry eye disease were recruited from o...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mary Ann Liebert, Inc., publishers
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6659741/ https://www.ncbi.nlm.nih.gov/pubmed/31259647 http://dx.doi.org/10.1089/jop.2018.0130 |
_version_ | 1783439190700064768 |
---|---|
author | Simsek, Cem Dogru, Murat Shinzawa, Megumi Den, Seika Kojima, Takashi Iseda, Hiroyuki Suzuki, Mai Shibasaki, Yoshiyuki Yoshida, Naoshi Shimazaki, Jun |
author_facet | Simsek, Cem Dogru, Murat Shinzawa, Megumi Den, Seika Kojima, Takashi Iseda, Hiroyuki Suzuki, Mai Shibasaki, Yoshiyuki Yoshida, Naoshi Shimazaki, Jun |
author_sort | Simsek, Cem |
collection | PubMed |
description | Purpose: To clarify the pharmacological effects of 2% rebamipide eye drops on mucosal membrane functions of the ocular surface epithelium, we investigated keratoconjunctival alterations at the cellular level in this study. Methods: Fifteen patients with definite dry eye disease were recruited from outpatient clinics of the Department of Ophthalmology, Ichikawa General Hospital. The patients received treatment with 2% rebamipide eye drops q.i.d for 12 weeks. Symptom score assessment, tear film breakup time, fluorescein and lissamine green ocular surface vital staining, grading of lid wiper epitheliopathy, Cochet–Bonnet corneal sensitivity, assessment of squamous metaplasia grades, and goblet cell density calculations from conjunctival impression cytology samples, as well as evaluation of nucleocytoplasmic ratios and corneal epithelial cells from in vivo confocal microscopy images before and 3 months after treatment were performed. Results: The mean symptom scores, tear film breakup time values, ocular surface fluorescein and lissamine green vital staining scores, and lid wiper scores showed a significant improvement after treatment (P < 0.01). The mean squamous metaplasia grade also showed a significant improvement (1.2 ± 0.1 → 0.3 ± 0.1) 3 months after treatment (P = 0.004). There were similar significant improvements in the mean corneal epithelial cell density (660.1 ± 62.6 → 1015.5 ± 43.5 cells/mm(2)) (P = 0.002) and nucleocytoplasmic ratios (0.1 ± 0.0 → 0.2 ± 0.0) (P = 0.0042) after treatment. Conclusions: Topical use of 2% rebamipide for 3 months was associated with improvements in ocular surface differentiation due to changes of mucosal functions at the cellular level. These alterations may explain objective and subjective improvements in dry eye disease. |
format | Online Article Text |
id | pubmed-6659741 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Mary Ann Liebert, Inc., publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-66597412019-07-29 The Efficacy of 2% Topical Rebamipide on Conjunctival Squamous Metaplasia and Goblet Cell Density in Dry Eye Disease Simsek, Cem Dogru, Murat Shinzawa, Megumi Den, Seika Kojima, Takashi Iseda, Hiroyuki Suzuki, Mai Shibasaki, Yoshiyuki Yoshida, Naoshi Shimazaki, Jun J Ocul Pharmacol Ther Original Articles Purpose: To clarify the pharmacological effects of 2% rebamipide eye drops on mucosal membrane functions of the ocular surface epithelium, we investigated keratoconjunctival alterations at the cellular level in this study. Methods: Fifteen patients with definite dry eye disease were recruited from outpatient clinics of the Department of Ophthalmology, Ichikawa General Hospital. The patients received treatment with 2% rebamipide eye drops q.i.d for 12 weeks. Symptom score assessment, tear film breakup time, fluorescein and lissamine green ocular surface vital staining, grading of lid wiper epitheliopathy, Cochet–Bonnet corneal sensitivity, assessment of squamous metaplasia grades, and goblet cell density calculations from conjunctival impression cytology samples, as well as evaluation of nucleocytoplasmic ratios and corneal epithelial cells from in vivo confocal microscopy images before and 3 months after treatment were performed. Results: The mean symptom scores, tear film breakup time values, ocular surface fluorescein and lissamine green vital staining scores, and lid wiper scores showed a significant improvement after treatment (P < 0.01). The mean squamous metaplasia grade also showed a significant improvement (1.2 ± 0.1 → 0.3 ± 0.1) 3 months after treatment (P = 0.004). There were similar significant improvements in the mean corneal epithelial cell density (660.1 ± 62.6 → 1015.5 ± 43.5 cells/mm(2)) (P = 0.002) and nucleocytoplasmic ratios (0.1 ± 0.0 → 0.2 ± 0.0) (P = 0.0042) after treatment. Conclusions: Topical use of 2% rebamipide for 3 months was associated with improvements in ocular surface differentiation due to changes of mucosal functions at the cellular level. These alterations may explain objective and subjective improvements in dry eye disease. Mary Ann Liebert, Inc., publishers 2019-07-01 2019-07-24 /pmc/articles/PMC6659741/ /pubmed/31259647 http://dx.doi.org/10.1089/jop.2018.0130 Text en © Cem Simsek et al. 2019; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are cited. |
spellingShingle | Original Articles Simsek, Cem Dogru, Murat Shinzawa, Megumi Den, Seika Kojima, Takashi Iseda, Hiroyuki Suzuki, Mai Shibasaki, Yoshiyuki Yoshida, Naoshi Shimazaki, Jun The Efficacy of 2% Topical Rebamipide on Conjunctival Squamous Metaplasia and Goblet Cell Density in Dry Eye Disease |
title | The Efficacy of 2% Topical Rebamipide on Conjunctival Squamous Metaplasia and Goblet Cell Density in Dry Eye Disease |
title_full | The Efficacy of 2% Topical Rebamipide on Conjunctival Squamous Metaplasia and Goblet Cell Density in Dry Eye Disease |
title_fullStr | The Efficacy of 2% Topical Rebamipide on Conjunctival Squamous Metaplasia and Goblet Cell Density in Dry Eye Disease |
title_full_unstemmed | The Efficacy of 2% Topical Rebamipide on Conjunctival Squamous Metaplasia and Goblet Cell Density in Dry Eye Disease |
title_short | The Efficacy of 2% Topical Rebamipide on Conjunctival Squamous Metaplasia and Goblet Cell Density in Dry Eye Disease |
title_sort | efficacy of 2% topical rebamipide on conjunctival squamous metaplasia and goblet cell density in dry eye disease |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6659741/ https://www.ncbi.nlm.nih.gov/pubmed/31259647 http://dx.doi.org/10.1089/jop.2018.0130 |
work_keys_str_mv | AT simsekcem theefficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT dogrumurat theefficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT shinzawamegumi theefficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT denseika theefficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT kojimatakashi theefficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT isedahiroyuki theefficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT suzukimai theefficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT shibasakiyoshiyuki theefficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT yoshidanaoshi theefficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT shimazakijun theefficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT simsekcem efficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT dogrumurat efficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT shinzawamegumi efficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT denseika efficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT kojimatakashi efficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT isedahiroyuki efficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT suzukimai efficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT shibasakiyoshiyuki efficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT yoshidanaoshi efficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease AT shimazakijun efficacyof2topicalrebamipideonconjunctivalsquamousmetaplasiaandgobletcelldensityindryeyedisease |