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Proteomic dataset: Profiling of cultivated Echerichia coli isolates from Crohn's disease patients and healthy individuals
One of the dysbioses often observed in Crohn's disease (CD) patients is an increased abundance of Escherichia coli (10–100 fold compared to healthy individuals) (Gevers et al., 2014). The data reported is a large-scale proteome profile for E. coli isolates collected from CD patients and healthy...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660468/ https://www.ncbi.nlm.nih.gov/pubmed/31372401 http://dx.doi.org/10.1016/j.dib.2019.103734 |
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author | Rakitina, Daria Baikova, Julia Pobeguts, Olga Bukato, Olga Butenko, Ivan Garanina, Irina Levites, Mark Manolov, Alexander Kanygina, Alexandra Kostryukova, Elena Semashko, Tatiana Karpova, Irina Babenko, Vladislav Scherbakov, Petr Khalif, Igor Shapina, Marina Parfenov, Asfold Ruchkina, Irina Knyazev, Oleg Fadeeva, Nina Subbotin, Alexandr Chamkin, Sergey Pyrkh, Alexandr Ivantsova, Marina Govorun, Vadim |
author_facet | Rakitina, Daria Baikova, Julia Pobeguts, Olga Bukato, Olga Butenko, Ivan Garanina, Irina Levites, Mark Manolov, Alexander Kanygina, Alexandra Kostryukova, Elena Semashko, Tatiana Karpova, Irina Babenko, Vladislav Scherbakov, Petr Khalif, Igor Shapina, Marina Parfenov, Asfold Ruchkina, Irina Knyazev, Oleg Fadeeva, Nina Subbotin, Alexandr Chamkin, Sergey Pyrkh, Alexandr Ivantsova, Marina Govorun, Vadim |
author_sort | Rakitina, Daria |
collection | PubMed |
description | One of the dysbioses often observed in Crohn's disease (CD) patients is an increased abundance of Escherichia coli (10–100 fold compared to healthy individuals) (Gevers et al., 2014). The data reported is a large-scale proteome profile for E. coli isolates collected from CD patients and healthy individuals. 43 isolates were achieved from 30 CD patients (17 male, 12 female, median age 30) and 19 isolates from 7 healthy individuals (7 male, median age 19). Isolates were cultivated on LB medium at aerobic conditions up to medium log phase. Protein extraction was performed with sodium deoxycholate (DCNa) and urea, alcylation with tris(2-carboxyethyl)phosphine and iodacetamide. Protein trypsinolysis was performed as described in (Matyushkina et al., 2016). Total cell proteomes were analysed by shotgun proteomics with HPLC-MS/MS on a maXis qTOF mass-spectrometer. The data including HPLC-MS/MS raw files and exported Mascot search results was deposited to the PRIDE repository project accession: PXD010920, project https://doi.org/10.6019/PXD010920. |
format | Online Article Text |
id | pubmed-6660468 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-66604682019-08-01 Proteomic dataset: Profiling of cultivated Echerichia coli isolates from Crohn's disease patients and healthy individuals Rakitina, Daria Baikova, Julia Pobeguts, Olga Bukato, Olga Butenko, Ivan Garanina, Irina Levites, Mark Manolov, Alexander Kanygina, Alexandra Kostryukova, Elena Semashko, Tatiana Karpova, Irina Babenko, Vladislav Scherbakov, Petr Khalif, Igor Shapina, Marina Parfenov, Asfold Ruchkina, Irina Knyazev, Oleg Fadeeva, Nina Subbotin, Alexandr Chamkin, Sergey Pyrkh, Alexandr Ivantsova, Marina Govorun, Vadim Data Brief Immunology and Microbiology One of the dysbioses often observed in Crohn's disease (CD) patients is an increased abundance of Escherichia coli (10–100 fold compared to healthy individuals) (Gevers et al., 2014). The data reported is a large-scale proteome profile for E. coli isolates collected from CD patients and healthy individuals. 43 isolates were achieved from 30 CD patients (17 male, 12 female, median age 30) and 19 isolates from 7 healthy individuals (7 male, median age 19). Isolates were cultivated on LB medium at aerobic conditions up to medium log phase. Protein extraction was performed with sodium deoxycholate (DCNa) and urea, alcylation with tris(2-carboxyethyl)phosphine and iodacetamide. Protein trypsinolysis was performed as described in (Matyushkina et al., 2016). Total cell proteomes were analysed by shotgun proteomics with HPLC-MS/MS on a maXis qTOF mass-spectrometer. The data including HPLC-MS/MS raw files and exported Mascot search results was deposited to the PRIDE repository project accession: PXD010920, project https://doi.org/10.6019/PXD010920. Elsevier 2019-03-07 /pmc/articles/PMC6660468/ /pubmed/31372401 http://dx.doi.org/10.1016/j.dib.2019.103734 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Immunology and Microbiology Rakitina, Daria Baikova, Julia Pobeguts, Olga Bukato, Olga Butenko, Ivan Garanina, Irina Levites, Mark Manolov, Alexander Kanygina, Alexandra Kostryukova, Elena Semashko, Tatiana Karpova, Irina Babenko, Vladislav Scherbakov, Petr Khalif, Igor Shapina, Marina Parfenov, Asfold Ruchkina, Irina Knyazev, Oleg Fadeeva, Nina Subbotin, Alexandr Chamkin, Sergey Pyrkh, Alexandr Ivantsova, Marina Govorun, Vadim Proteomic dataset: Profiling of cultivated Echerichia coli isolates from Crohn's disease patients and healthy individuals |
title | Proteomic dataset: Profiling of cultivated Echerichia coli isolates from Crohn's disease patients and healthy individuals |
title_full | Proteomic dataset: Profiling of cultivated Echerichia coli isolates from Crohn's disease patients and healthy individuals |
title_fullStr | Proteomic dataset: Profiling of cultivated Echerichia coli isolates from Crohn's disease patients and healthy individuals |
title_full_unstemmed | Proteomic dataset: Profiling of cultivated Echerichia coli isolates from Crohn's disease patients and healthy individuals |
title_short | Proteomic dataset: Profiling of cultivated Echerichia coli isolates from Crohn's disease patients and healthy individuals |
title_sort | proteomic dataset: profiling of cultivated echerichia coli isolates from crohn's disease patients and healthy individuals |
topic | Immunology and Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660468/ https://www.ncbi.nlm.nih.gov/pubmed/31372401 http://dx.doi.org/10.1016/j.dib.2019.103734 |
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