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From the Amazon: A comprehensive liver transcriptome dataset of the teleost fish tambaqui, Colossoma macropomum
The teleost fish tambaqui, Colossoma macropomum, is a valuable resource for the Brazillian aquaculture sector, representing more than one-quarter of the total production. In this context, the development of molecular tools is paramount to address and improve productivity, nutrition, and genetic bree...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660486/ https://www.ncbi.nlm.nih.gov/pubmed/31372415 http://dx.doi.org/10.1016/j.dib.2019.103751 |
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author | Machado, André M. Ferraz, Renato Ribeiro, Ricardo do Amaral Ozório, Rodrigo Castro, L. Filipe C. |
author_facet | Machado, André M. Ferraz, Renato Ribeiro, Ricardo do Amaral Ozório, Rodrigo Castro, L. Filipe C. |
author_sort | Machado, André M. |
collection | PubMed |
description | The teleost fish tambaqui, Colossoma macropomum, is a valuable resource for the Brazillian aquaculture sector, representing more than one-quarter of the total production. In this context, the development of molecular tools is paramount to address and improve productivity, nutrition, and genetic breeding programs. In this study, we applied RNA-seq technology to produce the first comprehensive liver transcriptome in this species. Our analysis generated a gold standard transcriptome with a total of 43,098 transcripts, with an N50 of 1855 bp and the average length of 1312 bp. To functionally annotate the transcripts, the Trinotate pipeline together with several public databases were scrutinized. The blast-x analysis revealed more than 40,000 homologous match hits for each database (NCBI-Nr, Uniref90, Swissprot, Trembl), while the Kaas web server allowed the mapping of our transcripts to 380 kegg pathways. The dataset provided in this study entails a comprehensive molecular resource, which will be instrumental to further develop tambaqui aquaculture, specifically in the field of nutrigenomics. |
format | Online Article Text |
id | pubmed-6660486 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-66604862019-08-01 From the Amazon: A comprehensive liver transcriptome dataset of the teleost fish tambaqui, Colossoma macropomum Machado, André M. Ferraz, Renato Ribeiro, Ricardo do Amaral Ozório, Rodrigo Castro, L. Filipe C. Data Brief Biochemistry, Genetics and Molecular Biology The teleost fish tambaqui, Colossoma macropomum, is a valuable resource for the Brazillian aquaculture sector, representing more than one-quarter of the total production. In this context, the development of molecular tools is paramount to address and improve productivity, nutrition, and genetic breeding programs. In this study, we applied RNA-seq technology to produce the first comprehensive liver transcriptome in this species. Our analysis generated a gold standard transcriptome with a total of 43,098 transcripts, with an N50 of 1855 bp and the average length of 1312 bp. To functionally annotate the transcripts, the Trinotate pipeline together with several public databases were scrutinized. The blast-x analysis revealed more than 40,000 homologous match hits for each database (NCBI-Nr, Uniref90, Swissprot, Trembl), while the Kaas web server allowed the mapping of our transcripts to 380 kegg pathways. The dataset provided in this study entails a comprehensive molecular resource, which will be instrumental to further develop tambaqui aquaculture, specifically in the field of nutrigenomics. Elsevier 2019-03-07 /pmc/articles/PMC6660486/ /pubmed/31372415 http://dx.doi.org/10.1016/j.dib.2019.103751 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Biochemistry, Genetics and Molecular Biology Machado, André M. Ferraz, Renato Ribeiro, Ricardo do Amaral Ozório, Rodrigo Castro, L. Filipe C. From the Amazon: A comprehensive liver transcriptome dataset of the teleost fish tambaqui, Colossoma macropomum |
title | From the Amazon: A comprehensive liver transcriptome dataset of the teleost fish tambaqui, Colossoma macropomum |
title_full | From the Amazon: A comprehensive liver transcriptome dataset of the teleost fish tambaqui, Colossoma macropomum |
title_fullStr | From the Amazon: A comprehensive liver transcriptome dataset of the teleost fish tambaqui, Colossoma macropomum |
title_full_unstemmed | From the Amazon: A comprehensive liver transcriptome dataset of the teleost fish tambaqui, Colossoma macropomum |
title_short | From the Amazon: A comprehensive liver transcriptome dataset of the teleost fish tambaqui, Colossoma macropomum |
title_sort | from the amazon: a comprehensive liver transcriptome dataset of the teleost fish tambaqui, colossoma macropomum |
topic | Biochemistry, Genetics and Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660486/ https://www.ncbi.nlm.nih.gov/pubmed/31372415 http://dx.doi.org/10.1016/j.dib.2019.103751 |
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