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From the Amazon: A comprehensive liver transcriptome dataset of the teleost fish tambaqui, Colossoma macropomum

The teleost fish tambaqui, Colossoma macropomum, is a valuable resource for the Brazillian aquaculture sector, representing more than one-quarter of the total production. In this context, the development of molecular tools is paramount to address and improve productivity, nutrition, and genetic bree...

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Autores principales: Machado, André M., Ferraz, Renato, Ribeiro, Ricardo do Amaral, Ozório, Rodrigo, Castro, L. Filipe C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660486/
https://www.ncbi.nlm.nih.gov/pubmed/31372415
http://dx.doi.org/10.1016/j.dib.2019.103751
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author Machado, André M.
Ferraz, Renato
Ribeiro, Ricardo do Amaral
Ozório, Rodrigo
Castro, L. Filipe C.
author_facet Machado, André M.
Ferraz, Renato
Ribeiro, Ricardo do Amaral
Ozório, Rodrigo
Castro, L. Filipe C.
author_sort Machado, André M.
collection PubMed
description The teleost fish tambaqui, Colossoma macropomum, is a valuable resource for the Brazillian aquaculture sector, representing more than one-quarter of the total production. In this context, the development of molecular tools is paramount to address and improve productivity, nutrition, and genetic breeding programs. In this study, we applied RNA-seq technology to produce the first comprehensive liver transcriptome in this species. Our analysis generated a gold standard transcriptome with a total of 43,098 transcripts, with an N50 of 1855 bp and the average length of 1312 bp. To functionally annotate the transcripts, the Trinotate pipeline together with several public databases were scrutinized. The blast-x analysis revealed more than 40,000 homologous match hits for each database (NCBI-Nr, Uniref90, Swissprot, Trembl), while the Kaas web server allowed the mapping of our transcripts to 380 kegg pathways. The dataset provided in this study entails a comprehensive molecular resource, which will be instrumental to further develop tambaqui aquaculture, specifically in the field of nutrigenomics.
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spelling pubmed-66604862019-08-01 From the Amazon: A comprehensive liver transcriptome dataset of the teleost fish tambaqui, Colossoma macropomum Machado, André M. Ferraz, Renato Ribeiro, Ricardo do Amaral Ozório, Rodrigo Castro, L. Filipe C. Data Brief Biochemistry, Genetics and Molecular Biology The teleost fish tambaqui, Colossoma macropomum, is a valuable resource for the Brazillian aquaculture sector, representing more than one-quarter of the total production. In this context, the development of molecular tools is paramount to address and improve productivity, nutrition, and genetic breeding programs. In this study, we applied RNA-seq technology to produce the first comprehensive liver transcriptome in this species. Our analysis generated a gold standard transcriptome with a total of 43,098 transcripts, with an N50 of 1855 bp and the average length of 1312 bp. To functionally annotate the transcripts, the Trinotate pipeline together with several public databases were scrutinized. The blast-x analysis revealed more than 40,000 homologous match hits for each database (NCBI-Nr, Uniref90, Swissprot, Trembl), while the Kaas web server allowed the mapping of our transcripts to 380 kegg pathways. The dataset provided in this study entails a comprehensive molecular resource, which will be instrumental to further develop tambaqui aquaculture, specifically in the field of nutrigenomics. Elsevier 2019-03-07 /pmc/articles/PMC6660486/ /pubmed/31372415 http://dx.doi.org/10.1016/j.dib.2019.103751 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Biochemistry, Genetics and Molecular Biology
Machado, André M.
Ferraz, Renato
Ribeiro, Ricardo do Amaral
Ozório, Rodrigo
Castro, L. Filipe C.
From the Amazon: A comprehensive liver transcriptome dataset of the teleost fish tambaqui, Colossoma macropomum
title From the Amazon: A comprehensive liver transcriptome dataset of the teleost fish tambaqui, Colossoma macropomum
title_full From the Amazon: A comprehensive liver transcriptome dataset of the teleost fish tambaqui, Colossoma macropomum
title_fullStr From the Amazon: A comprehensive liver transcriptome dataset of the teleost fish tambaqui, Colossoma macropomum
title_full_unstemmed From the Amazon: A comprehensive liver transcriptome dataset of the teleost fish tambaqui, Colossoma macropomum
title_short From the Amazon: A comprehensive liver transcriptome dataset of the teleost fish tambaqui, Colossoma macropomum
title_sort from the amazon: a comprehensive liver transcriptome dataset of the teleost fish tambaqui, colossoma macropomum
topic Biochemistry, Genetics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660486/
https://www.ncbi.nlm.nih.gov/pubmed/31372415
http://dx.doi.org/10.1016/j.dib.2019.103751
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