Cargando…

OVERFRAG: An overlapping DNA fragments generator for molecular cloning and synthetic biology

DNA synthesis and homologous recombination can be used to simplify molecular cloning and to make synthetic biology easily accessible (M.J. Czar et al., 2009). However, the design of overlapping DNA fragments to construct large molecules is time-consuming and requires verification of several paramete...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ferreira-Junior, Jose Ribamar, Digiampietri, Luciano A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2019
Materias:	Biochemistry, Genetics and Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660583/ https://www.ncbi.nlm.nih.gov/pubmed/31372451 http://dx.doi.org/10.1016/j.dib.2019.103806

Ejemplares similares

Optimization of overlap extension PCR for efficient transgene construction
por: Hilgarth, Roland S., et al.
Publicado: (2019)

Sequencing data of cell-free DNA fragments in living-related liver transplantation for inborn errors of metabolism
por: Zhu, Xiaofan, et al.
Publicado: (2020)

Air-drying of cells enables visualization of antiparallel microtubule overlaps in the spindle midzone
por: Ifuji, Aya, et al.
Publicado: (2018)

Molecular detection of human papillomavirus (HPV) in highly fragmented DNA from cervical cancer biopsies using double-nested PCR
por: Tawe, Leabaneng, et al.
Publicado: (2018)

A Python script to design primers for overlap extension PCR to ligate two DNA fragments
por: Hu, Yunzhuo, et al.
Publicado: (2022)

ABC cloning: An efficient, simple, and rapid restriction/ligase-free method
por: El Qaidi, Samir, et al.
Publicado: (2019)

Transcriptome dataset from bark and latex tissues of three Hevea brasiliensis clones
por: Abu Bakar, Mohd Fahmi, et al.
Publicado: (2020)

Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation
por: McCoy, Patrick J., et al.
Publicado: (2018)

RppH can faithfully replace TAP to allow cloning of 5′-triphosphate carrying small RNAs
por: Almeida, Miguel Vasconcelos, et al.
Publicado: (2019)

Synthetic dosage lethal (SDL) interaction data of Hmt1 arginine methyltransferase
por: Kyriakou, Dimitris, et al.
Publicado: (2020)

Tagging synthetic polymers with coumarin group for study nucleic acid interaction with gene delivery agents
por: Danilovtseva, Elena N., et al.
Publicado: (2019)

Dataset on the relation of synthetic super para magnetic nanoparticles coated with various electric charges and fibrillation of albumin protein
por: Javdani, Negin, et al.
Publicado: (2018)

Cell cycle progression data on human skin cancer cells with anticancer synthetic peptide LTX-315 treatment
por: Santa-González, Gloria A., et al.
Publicado: (2020)

Dataset of foraminiferal sedimentary DNA (sedDNA) sequences from Svalbard
por: Pawłowska, Joanna, et al.
Publicado: (2020)

Transfection of insect cell in suspension for efficient baculovirus generation
por: Roest, S., et al.
Publicado: (2016)

Developmental data for several human mitochondrial DNA (mtDNA) long amplification targets
por: Kavlick, Mark F.
Publicado: (2020)

Immunohistochemistry (IHC) staining of in-vitro cancer cell-generated tumoroids
por: Amereh, Meitham, et al.
Publicado: (2023)

Optimized method for methylated DNA immuno-precipitation
por: Guerrero-Bosagna, Carlos, et al.
Publicado: (2015)

An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae
por: Bresciani, Erica, et al.
Publicado: (2018)

Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase
por: Garafutdinov, Ravil R., et al.
Publicado: (2020)

Comparison and optimization for DNA extraction of archived fish specimens
por: Silva, Priscilla C., et al.
Publicado: (2019)

Extracting high-molecular weight DNA from cyanobacteria using Promega's Wizard(Ⓡ) HMW DNA extraction kit with a modified protocol, METIS
por: Hept, Megan A., et al.
Publicado: (2023)

A modified protocol for rapid DNA isolation from cotton (Gossypium spp.)
por: Ali, Qasim, et al.
Publicado: (2019)

Bioinformatic tools for tRNA gene analyses in mitochondrial DNA sequence data
por: Romanova, Elena V., et al.
Publicado: (2020)

18S rDNA sequencing data of benthic polychaetes from the Eastern Arabian Sea
por: Rengaiyan, Periasamy, et al.
Publicado: (2018)

Medical-grade buccal swabs versus drugstore cotton swabs: No difference in DNA yield
por: Ruiz, Cody A., et al.
Publicado: (2018)

Data on the activity of DNA methyltransferase in the uteri of CD-1 mice exposed to dibutyl phthalate
por: Colón-Díaz, Maricarmen, et al.
Publicado: (2019)

DNA-PAINT super-resolution imaging data of surface exposed active sites on particles
por: Delcanale, Pietro, et al.
Publicado: (2020)

A mixing heteroduplex mobility assay (mHMA) to genotype homozygous mutants with small indels generated by CRISPR-Cas9 nucleases
por: Foster, Samantha D., et al.
Publicado: (2018)

Data on assessment of safety and tear proteome change in response to orthokeratology lens – Insight from integrating clinical data and next generation proteomics
por: Tse, Jimmy Sung-Hei, et al.
Publicado: (2020)

Data on the target search by a single protein on DNA measured with ultrafast force-clamp spectroscopy
por: Monico, Carina, et al.
Publicado: (2019)

Comparative analysis and innovation of a simple and rapid method for high-quality RNA and DNA extraction of kiwifruit
por: Afshar-Mohammadian, Mansour, et al.
Publicado: (2018)

Data on likelihood ratios of two-person DNA mixtures interpreted using semi- and fully continuous systems
por: Rodriguez, Jae Joseph Russell B., et al.
Publicado: (2019)

A benchmark data set for the mechanical properties of double-stranded DNA and RNA under torsional constraint
por: Vanderlinden, Willem, et al.
Publicado: (2020)

Use of coagulation factor XIII (F13) gene as an internal control for normalization of genomic DNA’s for HLA typing
por: Neduvat, Anupama Cheleri, et al.
Publicado: (2018)

Evaluation of two independent protocols for the extraction of DNA and RNA from different tissues of sea cucumber Isostichopus badionotus
por: Puch-Hau, Carlos, et al.
Publicado: (2019)

Sequence variation data of the mitochondrial DNA D-loop region of the captive Malayan Gaur (Bos gaurus hubbacki)
por: Md-Zain, Badrul Munir, et al.
Publicado: (2018)

Genome-wide DNA methylation data from adult brain following prenatal immune activation and dietary intervention
por: Basil, Paul, et al.
Publicado: (2019)

Error-free and error-prone DNA repair gene expression data through reprogramming and passage in human iPS cells
por: Yoshimura, Yasuhide, et al.
Publicado: (2020)

Illumina and PacBio DNA sequencing data, de novo assembly and annotation of the genome of Aurantiochytrium limacinum strain CCAP_4062/1
por: Morabito, Christian, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_6cilmhihagpb8locu16h1g5k2h