Data on Quantitative Microbial Elemental Cycling (QMEC) primer design and validation

This work included the primer design details of Quantitative Microbial Elemental Cycling (QMEC) method, a high-throughput qPCR method for microbial carbon (C), nitrogen (N), phosphorus (P), sulfur (S) and methane metabolism potential detection and assessment. We designed 36 novel primers based on th...

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Detalles Bibliográficos
Autores principales: Zheng, Bang-Xiao, Ding, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Environmental Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660603/
https://www.ncbi.nlm.nih.gov/pubmed/31372463
http://dx.doi.org/10.1016/j.dib.2019.103820
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author Zheng, Bang-Xiao
Ding, Kai
author_facet Zheng, Bang-Xiao
Ding, Kai
author_sort Zheng, Bang-Xiao
collection PubMed
description This work included the primer design details of Quantitative Microbial Elemental Cycling (QMEC) method, a high-throughput qPCR method for microbial carbon (C), nitrogen (N), phosphorus (P), sulfur (S) and methane metabolism potential detection and assessment. We designed 36 novel primers based on their amino acid sequences. Via illumina sequencing technology, their phylogenetic taxonomy was identified and analyzed to validate the primer specificity.
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spelling pubmed-66606032019-08-01 Data on Quantitative Microbial Elemental Cycling (QMEC) primer design and validation Zheng, Bang-Xiao Ding, Kai Data Brief Environmental Science This work included the primer design details of Quantitative Microbial Elemental Cycling (QMEC) method, a high-throughput qPCR method for microbial carbon (C), nitrogen (N), phosphorus (P), sulfur (S) and methane metabolism potential detection and assessment. We designed 36 novel primers based on their amino acid sequences. Via illumina sequencing technology, their phylogenetic taxonomy was identified and analyzed to validate the primer specificity. Elsevier 2019-03-12 /pmc/articles/PMC6660603/ /pubmed/31372463 http://dx.doi.org/10.1016/j.dib.2019.103820 Text en © 2019 Published by Elsevier Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Environmental Science
Zheng, Bang-Xiao
Ding, Kai
Data on Quantitative Microbial Elemental Cycling (QMEC) primer design and validation
title Data on Quantitative Microbial Elemental Cycling (QMEC) primer design and validation
title_full Data on Quantitative Microbial Elemental Cycling (QMEC) primer design and validation
title_fullStr Data on Quantitative Microbial Elemental Cycling (QMEC) primer design and validation
title_full_unstemmed Data on Quantitative Microbial Elemental Cycling (QMEC) primer design and validation
title_short Data on Quantitative Microbial Elemental Cycling (QMEC) primer design and validation
title_sort data on quantitative microbial elemental cycling (qmec) primer design and validation
topic Environmental Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660603/
https://www.ncbi.nlm.nih.gov/pubmed/31372463
http://dx.doi.org/10.1016/j.dib.2019.103820
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