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Overexpression of chitotriosidase and YKL-40 in peripheral blood and sputum of healthy smokers and patients with chronic obstructive pulmonary disease
BACKGROUND: Despite the absence of endogenous chitin in humans, chitinases are present in the serum of healthy subjects and their levels are increased in a variety of chronic inflammatory conditions. It has been shown that chitotriosidase and structurally related chitinase-like protein-YKL-40 contri...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660640/ https://www.ncbi.nlm.nih.gov/pubmed/31413557 http://dx.doi.org/10.2147/COPD.S184097 |
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author | Majewski, Sebastian Tworek, Damian Szewczyk, Karolina Kiszałkiewicz, Justyna Kurmanowska, Zofia Brzeziańska-Lasota, Ewa Jerczyńska, Hanna Antczak, Adam Piotrowski, Wojciech Jerzy Górski, Paweł |
author_facet | Majewski, Sebastian Tworek, Damian Szewczyk, Karolina Kiszałkiewicz, Justyna Kurmanowska, Zofia Brzeziańska-Lasota, Ewa Jerczyńska, Hanna Antczak, Adam Piotrowski, Wojciech Jerzy Górski, Paweł |
author_sort | Majewski, Sebastian |
collection | PubMed |
description | BACKGROUND: Despite the absence of endogenous chitin in humans, chitinases are present in the serum of healthy subjects and their levels are increased in a variety of chronic inflammatory conditions. It has been shown that chitotriosidase and structurally related chitinase-like protein-YKL-40 contribute to the pathogenesis of COPD. However, details regarding the relation of their systemic and local airways levels remain unknown. OBJECTIVES: To examine peripheral blood and sputum chitotriosidase and YKL-40 expression in smokers and patients with COPD. METHODS: Forty patients with COPD, 20 healthy smokers and 10 healthy never-smokers were studied. Serum and induced sputum chitotriosidase protein and activity levels, YKL-40 concentrations, and their gene expression in sputum cells and peripheral blood mononuclear cells (PBMC) were evaluated. RESULTS: Both chitotriosidase protein levels and activity were higher in sputum obtained from COPD subjects compared to healthy never-smokers (P<0.05 and P<0.01, respectively). A similar pattern was observed for PBMC chitotriosidase mRNA expression (P<0.001). YKL-40 serum concentrations were elevated in healthy smokers and COPD subjects compared to healthy never-smokers (P<0.001 and P<0.01, respectively). In sputum, YKL-40 levels were increased in COPD compared to healthy never-smokers (P<0.01). PBMC YKL-40 mRNA expression was increased in COPD and healthy smokers compared to healthy never-smokers (P<0.0001). No associations were found between chitotriosidase or YKL-40 peripheral blood levels and sputum levels. CONCLUSIONS: Our results demonstrate that chitotriosidase and YKL-40 are overexpressed in peripheral blood and airways in both healthy smokers and COPD subjects which may indicate smoking-related activation of macrophages, neutrophils, and epithelial cells. |
format | Online Article Text |
id | pubmed-6660640 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-66606402019-08-14 Overexpression of chitotriosidase and YKL-40 in peripheral blood and sputum of healthy smokers and patients with chronic obstructive pulmonary disease Majewski, Sebastian Tworek, Damian Szewczyk, Karolina Kiszałkiewicz, Justyna Kurmanowska, Zofia Brzeziańska-Lasota, Ewa Jerczyńska, Hanna Antczak, Adam Piotrowski, Wojciech Jerzy Górski, Paweł Int J Chron Obstruct Pulmon Dis Original Research BACKGROUND: Despite the absence of endogenous chitin in humans, chitinases are present in the serum of healthy subjects and their levels are increased in a variety of chronic inflammatory conditions. It has been shown that chitotriosidase and structurally related chitinase-like protein-YKL-40 contribute to the pathogenesis of COPD. However, details regarding the relation of their systemic and local airways levels remain unknown. OBJECTIVES: To examine peripheral blood and sputum chitotriosidase and YKL-40 expression in smokers and patients with COPD. METHODS: Forty patients with COPD, 20 healthy smokers and 10 healthy never-smokers were studied. Serum and induced sputum chitotriosidase protein and activity levels, YKL-40 concentrations, and their gene expression in sputum cells and peripheral blood mononuclear cells (PBMC) were evaluated. RESULTS: Both chitotriosidase protein levels and activity were higher in sputum obtained from COPD subjects compared to healthy never-smokers (P<0.05 and P<0.01, respectively). A similar pattern was observed for PBMC chitotriosidase mRNA expression (P<0.001). YKL-40 serum concentrations were elevated in healthy smokers and COPD subjects compared to healthy never-smokers (P<0.001 and P<0.01, respectively). In sputum, YKL-40 levels were increased in COPD compared to healthy never-smokers (P<0.01). PBMC YKL-40 mRNA expression was increased in COPD and healthy smokers compared to healthy never-smokers (P<0.0001). No associations were found between chitotriosidase or YKL-40 peripheral blood levels and sputum levels. CONCLUSIONS: Our results demonstrate that chitotriosidase and YKL-40 are overexpressed in peripheral blood and airways in both healthy smokers and COPD subjects which may indicate smoking-related activation of macrophages, neutrophils, and epithelial cells. Dove 2019-07-22 /pmc/articles/PMC6660640/ /pubmed/31413557 http://dx.doi.org/10.2147/COPD.S184097 Text en © 2019 Majewski et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Majewski, Sebastian Tworek, Damian Szewczyk, Karolina Kiszałkiewicz, Justyna Kurmanowska, Zofia Brzeziańska-Lasota, Ewa Jerczyńska, Hanna Antczak, Adam Piotrowski, Wojciech Jerzy Górski, Paweł Overexpression of chitotriosidase and YKL-40 in peripheral blood and sputum of healthy smokers and patients with chronic obstructive pulmonary disease |
title | Overexpression of chitotriosidase and YKL-40 in peripheral blood and sputum of healthy smokers and patients with chronic obstructive pulmonary disease |
title_full | Overexpression of chitotriosidase and YKL-40 in peripheral blood and sputum of healthy smokers and patients with chronic obstructive pulmonary disease |
title_fullStr | Overexpression of chitotriosidase and YKL-40 in peripheral blood and sputum of healthy smokers and patients with chronic obstructive pulmonary disease |
title_full_unstemmed | Overexpression of chitotriosidase and YKL-40 in peripheral blood and sputum of healthy smokers and patients with chronic obstructive pulmonary disease |
title_short | Overexpression of chitotriosidase and YKL-40 in peripheral blood and sputum of healthy smokers and patients with chronic obstructive pulmonary disease |
title_sort | overexpression of chitotriosidase and ykl-40 in peripheral blood and sputum of healthy smokers and patients with chronic obstructive pulmonary disease |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660640/ https://www.ncbi.nlm.nih.gov/pubmed/31413557 http://dx.doi.org/10.2147/COPD.S184097 |
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