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Dok-3 deficient mice display different immune clustering and Tim-3 expression
BACKGROUND: Dok-3 has been shown to play an important role in immune system. Tim-3 also has been recognized as an important immune regulator which involves in many diseases. The relationship of them is still unclear. METHODS: We detected the expression of Tim-3 on spleen immune cells from Dok-3 defi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660655/ https://www.ncbi.nlm.nih.gov/pubmed/31351483 http://dx.doi.org/10.1186/s40001-019-0384-7 |
Sumario: | BACKGROUND: Dok-3 has been shown to play an important role in immune system. Tim-3 also has been recognized as an important immune regulator which involves in many diseases. The relationship of them is still unclear. METHODS: We detected the expression of Tim-3 on spleen immune cells from Dok-3 deficient mice and control mice by flow cytometry. RESULTS: In this article, we found that Dok-3(−/−) mice display almost entirely different immune clustering characteristics compared with wild type 129 mice. The CD4 T cells and CD8 T cells decreased and DC cells, macrophages, MDSCs increased when the Dok-3 gene knocked-out. The Tim-3 expression on CD4 T cells, CD8 T cells, NK cells, DC cells increased when the Dok-3 gene knocked-out. The macrophages and MDSCs just display the opposite results. CONCLUSIONS: Although Dok-3(−/−) mice display different immune clustering and Tim-3 expression, the mechanism still needs further study. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s40001-019-0384-7) contains supplementary material, which is available to authorized users. |
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