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Dok-3 deficient mice display different immune clustering and Tim-3 expression

BACKGROUND: Dok-3 has been shown to play an important role in immune system. Tim-3 also has been recognized as an important immune regulator which involves in many diseases. The relationship of them is still unclear. METHODS: We detected the expression of Tim-3 on spleen immune cells from Dok-3 defi...

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Autores principales: Yan, Wenjiang, Tian, Yijia, Sun, Peng, Yang, Jingjing, Li, Na, Sun, Yuanyuan, Wang, Shuangxi, Zhang, Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660655/
https://www.ncbi.nlm.nih.gov/pubmed/31351483
http://dx.doi.org/10.1186/s40001-019-0384-7
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author Yan, Wenjiang
Tian, Yijia
Sun, Peng
Yang, Jingjing
Li, Na
Sun, Yuanyuan
Wang, Shuangxi
Zhang, Cheng
author_facet Yan, Wenjiang
Tian, Yijia
Sun, Peng
Yang, Jingjing
Li, Na
Sun, Yuanyuan
Wang, Shuangxi
Zhang, Cheng
author_sort Yan, Wenjiang
collection PubMed
description BACKGROUND: Dok-3 has been shown to play an important role in immune system. Tim-3 also has been recognized as an important immune regulator which involves in many diseases. The relationship of them is still unclear. METHODS: We detected the expression of Tim-3 on spleen immune cells from Dok-3 deficient mice and control mice by flow cytometry. RESULTS: In this article, we found that Dok-3(−/−) mice display almost entirely different immune clustering characteristics compared with wild type 129 mice. The CD4 T cells and CD8 T cells decreased and DC cells, macrophages, MDSCs increased when the Dok-3 gene knocked-out. The Tim-3 expression on CD4 T cells, CD8 T cells, NK cells, DC cells increased when the Dok-3 gene knocked-out. The macrophages and MDSCs just display the opposite results. CONCLUSIONS: Although Dok-3(−/−) mice display different immune clustering and Tim-3 expression, the mechanism still needs further study. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s40001-019-0384-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-66606552019-08-01 Dok-3 deficient mice display different immune clustering and Tim-3 expression Yan, Wenjiang Tian, Yijia Sun, Peng Yang, Jingjing Li, Na Sun, Yuanyuan Wang, Shuangxi Zhang, Cheng Eur J Med Res Research BACKGROUND: Dok-3 has been shown to play an important role in immune system. Tim-3 also has been recognized as an important immune regulator which involves in many diseases. The relationship of them is still unclear. METHODS: We detected the expression of Tim-3 on spleen immune cells from Dok-3 deficient mice and control mice by flow cytometry. RESULTS: In this article, we found that Dok-3(−/−) mice display almost entirely different immune clustering characteristics compared with wild type 129 mice. The CD4 T cells and CD8 T cells decreased and DC cells, macrophages, MDSCs increased when the Dok-3 gene knocked-out. The Tim-3 expression on CD4 T cells, CD8 T cells, NK cells, DC cells increased when the Dok-3 gene knocked-out. The macrophages and MDSCs just display the opposite results. CONCLUSIONS: Although Dok-3(−/−) mice display different immune clustering and Tim-3 expression, the mechanism still needs further study. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s40001-019-0384-7) contains supplementary material, which is available to authorized users. BioMed Central 2019-07-27 /pmc/articles/PMC6660655/ /pubmed/31351483 http://dx.doi.org/10.1186/s40001-019-0384-7 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Yan, Wenjiang
Tian, Yijia
Sun, Peng
Yang, Jingjing
Li, Na
Sun, Yuanyuan
Wang, Shuangxi
Zhang, Cheng
Dok-3 deficient mice display different immune clustering and Tim-3 expression
title Dok-3 deficient mice display different immune clustering and Tim-3 expression
title_full Dok-3 deficient mice display different immune clustering and Tim-3 expression
title_fullStr Dok-3 deficient mice display different immune clustering and Tim-3 expression
title_full_unstemmed Dok-3 deficient mice display different immune clustering and Tim-3 expression
title_short Dok-3 deficient mice display different immune clustering and Tim-3 expression
title_sort dok-3 deficient mice display different immune clustering and tim-3 expression
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660655/
https://www.ncbi.nlm.nih.gov/pubmed/31351483
http://dx.doi.org/10.1186/s40001-019-0384-7
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