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The Lentiviral Vector Pseudotyped by Modified Rabies Glycoprotein Does Not Cause Reactive Gliosis and Neurodegeneration in Rat Hippocampus

BACKGROUND: A human immunodeficiency virus type 1 (HIV-1)-based lentiviral vector (LV) pseudotyped by a variant of rabies envelope glycoprotein, FUG-B2, has previously been prepared and used in transfection of hippocampal CA1 ("Cornu Ammonis" area 1) neurons. This study aimed to verify rea...

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Autores principales: Farzaneh, Mostafa, Sayyah, Mohammad, Eshraghi, Hamid Reza, Panahi, Negar, Mirzapourdelavar, Hadi, Gholami Pourbadie, Hamid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pasteur Institute of Iran 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6661131/
https://www.ncbi.nlm.nih.gov/pubmed/31103020
http://dx.doi.org/10.29252/.23.5.324
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author Farzaneh, Mostafa
Sayyah, Mohammad
Eshraghi, Hamid Reza
Panahi, Negar
Mirzapourdelavar, Hadi
Gholami Pourbadie, Hamid
author_facet Farzaneh, Mostafa
Sayyah, Mohammad
Eshraghi, Hamid Reza
Panahi, Negar
Mirzapourdelavar, Hadi
Gholami Pourbadie, Hamid
author_sort Farzaneh, Mostafa
collection PubMed
description BACKGROUND: A human immunodeficiency virus type 1 (HIV-1)-based lentiviral vector (LV) pseudotyped by a variant of rabies envelope glycoprotein, FUG-B2, has previously been prepared and used in transfection of hippocampal CA1 ("Cornu Ammonis" area 1) neurons. This study aimed to verify reactive gliosis and neuronal damage after injection of the vector into the rat hippocampus. METHODS: HEK 293T cells were transfected with transfer (fck-Jaws-GFP-ER2), envelope (FUG-B2), and packaging (pMDLg/pRRE, pRSV-Rev) plasmids, and the vector was injected into CA1 of the rat hippocampus. After one week, transduction efficiency, and the number of neuronal and astroglial cells were determined in CA1 and CA3 by double staining of the brain slices. RESULTS: Hippocampal cells were successfully transfected as 92.7% of CA1 and 95.8% of CA3 neuronal cells expressed GFP. The frequency of neuronal and astroglial cells in CA1 and CA3 of the vector-injected rats remained unchanged compared to those in the control and the saline-injected rats. Furthermore, no morphological change was found in hippocampal astrocytes and neuronal cells. CONCLUSION: The HIV-1-based LV pseudotyped by FUG-B2 is safe and does not cause neuroinflammation and neuronal loss once directly delivered into the rat hippocampus.  
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spelling pubmed-66611312019-09-01 The Lentiviral Vector Pseudotyped by Modified Rabies Glycoprotein Does Not Cause Reactive Gliosis and Neurodegeneration in Rat Hippocampus Farzaneh, Mostafa Sayyah, Mohammad Eshraghi, Hamid Reza Panahi, Negar Mirzapourdelavar, Hadi Gholami Pourbadie, Hamid Iran Biomed J Full Length BACKGROUND: A human immunodeficiency virus type 1 (HIV-1)-based lentiviral vector (LV) pseudotyped by a variant of rabies envelope glycoprotein, FUG-B2, has previously been prepared and used in transfection of hippocampal CA1 ("Cornu Ammonis" area 1) neurons. This study aimed to verify reactive gliosis and neuronal damage after injection of the vector into the rat hippocampus. METHODS: HEK 293T cells were transfected with transfer (fck-Jaws-GFP-ER2), envelope (FUG-B2), and packaging (pMDLg/pRRE, pRSV-Rev) plasmids, and the vector was injected into CA1 of the rat hippocampus. After one week, transduction efficiency, and the number of neuronal and astroglial cells were determined in CA1 and CA3 by double staining of the brain slices. RESULTS: Hippocampal cells were successfully transfected as 92.7% of CA1 and 95.8% of CA3 neuronal cells expressed GFP. The frequency of neuronal and astroglial cells in CA1 and CA3 of the vector-injected rats remained unchanged compared to those in the control and the saline-injected rats. Furthermore, no morphological change was found in hippocampal astrocytes and neuronal cells. CONCLUSION: The HIV-1-based LV pseudotyped by FUG-B2 is safe and does not cause neuroinflammation and neuronal loss once directly delivered into the rat hippocampus.   Pasteur Institute of Iran 2019-09 /pmc/articles/PMC6661131/ /pubmed/31103020 http://dx.doi.org/10.29252/.23.5.324 Text en © Iranian Biomedical Journal This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Length
Farzaneh, Mostafa
Sayyah, Mohammad
Eshraghi, Hamid Reza
Panahi, Negar
Mirzapourdelavar, Hadi
Gholami Pourbadie, Hamid
The Lentiviral Vector Pseudotyped by Modified Rabies Glycoprotein Does Not Cause Reactive Gliosis and Neurodegeneration in Rat Hippocampus
title The Lentiviral Vector Pseudotyped by Modified Rabies Glycoprotein Does Not Cause Reactive Gliosis and Neurodegeneration in Rat Hippocampus
title_full The Lentiviral Vector Pseudotyped by Modified Rabies Glycoprotein Does Not Cause Reactive Gliosis and Neurodegeneration in Rat Hippocampus
title_fullStr The Lentiviral Vector Pseudotyped by Modified Rabies Glycoprotein Does Not Cause Reactive Gliosis and Neurodegeneration in Rat Hippocampus
title_full_unstemmed The Lentiviral Vector Pseudotyped by Modified Rabies Glycoprotein Does Not Cause Reactive Gliosis and Neurodegeneration in Rat Hippocampus
title_short The Lentiviral Vector Pseudotyped by Modified Rabies Glycoprotein Does Not Cause Reactive Gliosis and Neurodegeneration in Rat Hippocampus
title_sort lentiviral vector pseudotyped by modified rabies glycoprotein does not cause reactive gliosis and neurodegeneration in rat hippocampus
topic Full Length
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6661131/
https://www.ncbi.nlm.nih.gov/pubmed/31103020
http://dx.doi.org/10.29252/.23.5.324
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