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Glycolate from microalgae: an efficient carbon source for biotechnological applications
Glycolate is produced in autotrophic cells under high temperatures and C(i)‐limitation via oxygenation of ribulose‐1,5‐bisphosphate. In unicellular algae, glycolate is lost via excretion or metabolized via the C(2) cycle by consuming reductants, ATP and CO (2) emission (photorespiration). Therefore,...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662103/ https://www.ncbi.nlm.nih.gov/pubmed/30637910 http://dx.doi.org/10.1111/pbi.13078 |
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author | Taubert, Anja Jakob, Torsten Wilhelm, Christian |
author_facet | Taubert, Anja Jakob, Torsten Wilhelm, Christian |
author_sort | Taubert, Anja |
collection | PubMed |
description | Glycolate is produced in autotrophic cells under high temperatures and C(i)‐limitation via oxygenation of ribulose‐1,5‐bisphosphate. In unicellular algae, glycolate is lost via excretion or metabolized via the C(2) cycle by consuming reductants, ATP and CO (2) emission (photorespiration). Therefore, photorespiration is an inhibitory process for biomass production. However, cells can be manipulated in a way that they become glycolate‐producing ‘cell factories’, when the ratio carboxylation/oxygenation is 2. If under these conditions the C(2) cycle is blocked, glycolate excretion becomes the only pathway of photosynthetic carbon flow. The study aims to proof the biotechnological applicability of algal‐based glycolate excretion as a new biotechnological platform. It is shown that cells of Chlamydomonas can be cultivated under specific conditions to establish a constant and long‐term stable glycolate excretion during the light phase. The cultures achieved a high efficiency of 82% of assimilated carbon transferred into glycolate biosynthesis without losses of function in cell vitality. Moreover, the glycolate accumulation in the medium is high enough to be directly used for microbial fermentation but does not show toxic effects to the glycolate‐producing cells. |
format | Online Article Text |
id | pubmed-6662103 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-66621032019-08-05 Glycolate from microalgae: an efficient carbon source for biotechnological applications Taubert, Anja Jakob, Torsten Wilhelm, Christian Plant Biotechnol J Research Articles Glycolate is produced in autotrophic cells under high temperatures and C(i)‐limitation via oxygenation of ribulose‐1,5‐bisphosphate. In unicellular algae, glycolate is lost via excretion or metabolized via the C(2) cycle by consuming reductants, ATP and CO (2) emission (photorespiration). Therefore, photorespiration is an inhibitory process for biomass production. However, cells can be manipulated in a way that they become glycolate‐producing ‘cell factories’, when the ratio carboxylation/oxygenation is 2. If under these conditions the C(2) cycle is blocked, glycolate excretion becomes the only pathway of photosynthetic carbon flow. The study aims to proof the biotechnological applicability of algal‐based glycolate excretion as a new biotechnological platform. It is shown that cells of Chlamydomonas can be cultivated under specific conditions to establish a constant and long‐term stable glycolate excretion during the light phase. The cultures achieved a high efficiency of 82% of assimilated carbon transferred into glycolate biosynthesis without losses of function in cell vitality. Moreover, the glycolate accumulation in the medium is high enough to be directly used for microbial fermentation but does not show toxic effects to the glycolate‐producing cells. John Wiley and Sons Inc. 2019-02-04 2019-08 /pmc/articles/PMC6662103/ /pubmed/30637910 http://dx.doi.org/10.1111/pbi.13078 Text en © 2019 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Taubert, Anja Jakob, Torsten Wilhelm, Christian Glycolate from microalgae: an efficient carbon source for biotechnological applications |
title | Glycolate from microalgae: an efficient carbon source for biotechnological applications |
title_full | Glycolate from microalgae: an efficient carbon source for biotechnological applications |
title_fullStr | Glycolate from microalgae: an efficient carbon source for biotechnological applications |
title_full_unstemmed | Glycolate from microalgae: an efficient carbon source for biotechnological applications |
title_short | Glycolate from microalgae: an efficient carbon source for biotechnological applications |
title_sort | glycolate from microalgae: an efficient carbon source for biotechnological applications |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662103/ https://www.ncbi.nlm.nih.gov/pubmed/30637910 http://dx.doi.org/10.1111/pbi.13078 |
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