Cargando…

EA at PC6 Promotes Gastric Motility: Role of Brainstem Vagovagal Neurocircuits

BACKGROUND: We aimed to assess whether electroacupuncture (EA) at PC6 affects gastric motility via the vagovagal reflex and if so whether brainstem vagovagal neurocircuits and related transmitters are involved. METHODS: Gastric motility was measured in male Sprague-Dawley (SD) rats by placing a smal...

Descripción completa

Detalles Bibliográficos
Autores principales: Lu, Mengjiang, Chen, Chienchih, Li, Wen, Yu, Zhi, Xu, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662446/
https://www.ncbi.nlm.nih.gov/pubmed/31379967
http://dx.doi.org/10.1155/2019/7457485
_version_ 1783439654189531136
author Lu, Mengjiang
Chen, Chienchih
Li, Wen
Yu, Zhi
Xu, Bin
author_facet Lu, Mengjiang
Chen, Chienchih
Li, Wen
Yu, Zhi
Xu, Bin
author_sort Lu, Mengjiang
collection PubMed
description BACKGROUND: We aimed to assess whether electroacupuncture (EA) at PC6 affects gastric motility via the vagovagal reflex and if so whether brainstem vagovagal neurocircuits and related transmitters are involved. METHODS: Gastric motility was measured in male Sprague-Dawley (SD) rats by placing a small manometric balloon in the gastric antrum. The rats were subjected to control, sham surgery, vagotomy, sympathectomy, and microinjection group, including artificial cerebrospinal fluid, gamma-aminobutyric acid (GABA), and glutamic acid (L-Glu). The effect of EA at PC6 on gastric motility was measured. Moreover, electrophysiological testing was used to measure the effect of EA at PC6 on the parasympathetic and sympathetic nerves. In addition, artificial cerebrospinal fluid, L-Glu, and GABA have been microinjected into the dorsal motor nucleus of the vagus (DMV) to measure the changes in gastric motility and parasympathetic nerve discharge induced by EA at PC6. KEY RESULTS: EA facilitated the gastric motility in control group. In the vagotomy group, gastric motility was not affected by EA at PC6. However, in the sympathectomy group, gastric motility was similar to control group. Acupuncture at PC6 increased parasympathetic nerve discharge but not sympathetic nerve discharge. Furthermore, the microinjection of L-Glu into the DMV increased gastric motility, although EA at PC6 showed no remarkable change in this group. The injection of GABA reduced gastric motility and parasympathetic nerve discharge, but EA at PC6 significantly increased gastric motility and the parasympathetic nerve discharge in this group. CONCLUSIONS AND INFERENCES: EA at PC6—primarily by inhibiting GABA transmission to DMV—reduced the inhibition of efferent vagal motor fibers and thus promoted efferent vagus nerve activity and increased gastric motility.
format Online
Article
Text
id pubmed-6662446
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-66624462019-08-04 EA at PC6 Promotes Gastric Motility: Role of Brainstem Vagovagal Neurocircuits Lu, Mengjiang Chen, Chienchih Li, Wen Yu, Zhi Xu, Bin Evid Based Complement Alternat Med Research Article BACKGROUND: We aimed to assess whether electroacupuncture (EA) at PC6 affects gastric motility via the vagovagal reflex and if so whether brainstem vagovagal neurocircuits and related transmitters are involved. METHODS: Gastric motility was measured in male Sprague-Dawley (SD) rats by placing a small manometric balloon in the gastric antrum. The rats were subjected to control, sham surgery, vagotomy, sympathectomy, and microinjection group, including artificial cerebrospinal fluid, gamma-aminobutyric acid (GABA), and glutamic acid (L-Glu). The effect of EA at PC6 on gastric motility was measured. Moreover, electrophysiological testing was used to measure the effect of EA at PC6 on the parasympathetic and sympathetic nerves. In addition, artificial cerebrospinal fluid, L-Glu, and GABA have been microinjected into the dorsal motor nucleus of the vagus (DMV) to measure the changes in gastric motility and parasympathetic nerve discharge induced by EA at PC6. KEY RESULTS: EA facilitated the gastric motility in control group. In the vagotomy group, gastric motility was not affected by EA at PC6. However, in the sympathectomy group, gastric motility was similar to control group. Acupuncture at PC6 increased parasympathetic nerve discharge but not sympathetic nerve discharge. Furthermore, the microinjection of L-Glu into the DMV increased gastric motility, although EA at PC6 showed no remarkable change in this group. The injection of GABA reduced gastric motility and parasympathetic nerve discharge, but EA at PC6 significantly increased gastric motility and the parasympathetic nerve discharge in this group. CONCLUSIONS AND INFERENCES: EA at PC6—primarily by inhibiting GABA transmission to DMV—reduced the inhibition of efferent vagal motor fibers and thus promoted efferent vagus nerve activity and increased gastric motility. Hindawi 2019-07-15 /pmc/articles/PMC6662446/ /pubmed/31379967 http://dx.doi.org/10.1155/2019/7457485 Text en Copyright © 2019 Mengjiang Lu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lu, Mengjiang
Chen, Chienchih
Li, Wen
Yu, Zhi
Xu, Bin
EA at PC6 Promotes Gastric Motility: Role of Brainstem Vagovagal Neurocircuits
title EA at PC6 Promotes Gastric Motility: Role of Brainstem Vagovagal Neurocircuits
title_full EA at PC6 Promotes Gastric Motility: Role of Brainstem Vagovagal Neurocircuits
title_fullStr EA at PC6 Promotes Gastric Motility: Role of Brainstem Vagovagal Neurocircuits
title_full_unstemmed EA at PC6 Promotes Gastric Motility: Role of Brainstem Vagovagal Neurocircuits
title_short EA at PC6 Promotes Gastric Motility: Role of Brainstem Vagovagal Neurocircuits
title_sort ea at pc6 promotes gastric motility: role of brainstem vagovagal neurocircuits
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662446/
https://www.ncbi.nlm.nih.gov/pubmed/31379967
http://dx.doi.org/10.1155/2019/7457485
work_keys_str_mv AT lumengjiang eaatpc6promotesgastricmotilityroleofbrainstemvagovagalneurocircuits
AT chenchienchih eaatpc6promotesgastricmotilityroleofbrainstemvagovagalneurocircuits
AT liwen eaatpc6promotesgastricmotilityroleofbrainstemvagovagalneurocircuits
AT yuzhi eaatpc6promotesgastricmotilityroleofbrainstemvagovagalneurocircuits
AT xubin eaatpc6promotesgastricmotilityroleofbrainstemvagovagalneurocircuits