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Disruption of gene SPL35, encoding a novel CUE domain‐containing protein, leads to cell death and enhanced disease response in rice

Lesion mimic mutants that exhibit spontaneous hypersensitive response (HR)‐like necrotic lesions are ideal experimental systems for elucidating molecular mechanisms involved in plant cell death and defence responses. Here we report identification of a rice lesion mimic mutant, spotted leaf 35 (spl35...

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Autores principales: Ma, Jian, Wang, Yongfei, Ma, Xiaoding, Meng, Lingzhi, Jing, Ruonan, Wang, Fan, Wang, Shuai, Cheng, Zhijun, Zhang, Xin, Jiang, Ling, Wang, Jiulin, Wang, Jie, Zhao, Zhichao, Guo, Xiuping, Lin, Qibing, Wu, Fuqing, Zhu, Shanshan, Wu, Chuanyin, Ren, Yulong, Lei, Cailin, Zhai, Huqu, Wan, Jianmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662554/
https://www.ncbi.nlm.nih.gov/pubmed/30771255
http://dx.doi.org/10.1111/pbi.13093
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author Ma, Jian
Wang, Yongfei
Ma, Xiaoding
Meng, Lingzhi
Jing, Ruonan
Wang, Fan
Wang, Shuai
Cheng, Zhijun
Zhang, Xin
Jiang, Ling
Wang, Jiulin
Wang, Jie
Zhao, Zhichao
Guo, Xiuping
Lin, Qibing
Wu, Fuqing
Zhu, Shanshan
Wu, Chuanyin
Ren, Yulong
Lei, Cailin
Zhai, Huqu
Wan, Jianmin
author_facet Ma, Jian
Wang, Yongfei
Ma, Xiaoding
Meng, Lingzhi
Jing, Ruonan
Wang, Fan
Wang, Shuai
Cheng, Zhijun
Zhang, Xin
Jiang, Ling
Wang, Jiulin
Wang, Jie
Zhao, Zhichao
Guo, Xiuping
Lin, Qibing
Wu, Fuqing
Zhu, Shanshan
Wu, Chuanyin
Ren, Yulong
Lei, Cailin
Zhai, Huqu
Wan, Jianmin
author_sort Ma, Jian
collection PubMed
description Lesion mimic mutants that exhibit spontaneous hypersensitive response (HR)‐like necrotic lesions are ideal experimental systems for elucidating molecular mechanisms involved in plant cell death and defence responses. Here we report identification of a rice lesion mimic mutant, spotted leaf 35 (spl35), and cloning of the causal gene by TAIL‐PCR strategy. spl35 exhibited decreased chlorophyll content, higher accumulation of H(2)O(2), up‐regulated expression of defence‐related marker genes, and enhanced resistance to both fungal and bacterial pathogens of rice. The SPL35 gene encodes a novel CUE (coupling of ubiquitin conjugation to ER degradation) domain‐containing protein that is predominantly localized in cytosol, ER and unknown punctate compartment(s). SPL35 is constitutively expressed in all organs, and both overexpression and knockdown of SPL35 cause the lesion mimic phenotype. SPL35 directly interacts with the E2 protein OsUBC5a and the coatomer subunit delta proteins Delta‐COP1 and Delta‐COP2 through the CUE domain, and down‐regulation of these interacting proteins also cause development of HR‐like lesions resembling those in spl35 and activation of defence responses, indicating that SPL35 may be involved in the ubiquitination and vesicular trafficking pathways. Our findings provide insight into a role of SPL35 in regulating cell death and defence response in plants.
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spelling pubmed-66625542019-08-05 Disruption of gene SPL35, encoding a novel CUE domain‐containing protein, leads to cell death and enhanced disease response in rice Ma, Jian Wang, Yongfei Ma, Xiaoding Meng, Lingzhi Jing, Ruonan Wang, Fan Wang, Shuai Cheng, Zhijun Zhang, Xin Jiang, Ling Wang, Jiulin Wang, Jie Zhao, Zhichao Guo, Xiuping Lin, Qibing Wu, Fuqing Zhu, Shanshan Wu, Chuanyin Ren, Yulong Lei, Cailin Zhai, Huqu Wan, Jianmin Plant Biotechnol J Research Articles Lesion mimic mutants that exhibit spontaneous hypersensitive response (HR)‐like necrotic lesions are ideal experimental systems for elucidating molecular mechanisms involved in plant cell death and defence responses. Here we report identification of a rice lesion mimic mutant, spotted leaf 35 (spl35), and cloning of the causal gene by TAIL‐PCR strategy. spl35 exhibited decreased chlorophyll content, higher accumulation of H(2)O(2), up‐regulated expression of defence‐related marker genes, and enhanced resistance to both fungal and bacterial pathogens of rice. The SPL35 gene encodes a novel CUE (coupling of ubiquitin conjugation to ER degradation) domain‐containing protein that is predominantly localized in cytosol, ER and unknown punctate compartment(s). SPL35 is constitutively expressed in all organs, and both overexpression and knockdown of SPL35 cause the lesion mimic phenotype. SPL35 directly interacts with the E2 protein OsUBC5a and the coatomer subunit delta proteins Delta‐COP1 and Delta‐COP2 through the CUE domain, and down‐regulation of these interacting proteins also cause development of HR‐like lesions resembling those in spl35 and activation of defence responses, indicating that SPL35 may be involved in the ubiquitination and vesicular trafficking pathways. Our findings provide insight into a role of SPL35 in regulating cell death and defence response in plants. John Wiley and Sons Inc. 2019-03-05 2019-08 /pmc/articles/PMC6662554/ /pubmed/30771255 http://dx.doi.org/10.1111/pbi.13093 Text en © 2019 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Ma, Jian
Wang, Yongfei
Ma, Xiaoding
Meng, Lingzhi
Jing, Ruonan
Wang, Fan
Wang, Shuai
Cheng, Zhijun
Zhang, Xin
Jiang, Ling
Wang, Jiulin
Wang, Jie
Zhao, Zhichao
Guo, Xiuping
Lin, Qibing
Wu, Fuqing
Zhu, Shanshan
Wu, Chuanyin
Ren, Yulong
Lei, Cailin
Zhai, Huqu
Wan, Jianmin
Disruption of gene SPL35, encoding a novel CUE domain‐containing protein, leads to cell death and enhanced disease response in rice
title Disruption of gene SPL35, encoding a novel CUE domain‐containing protein, leads to cell death and enhanced disease response in rice
title_full Disruption of gene SPL35, encoding a novel CUE domain‐containing protein, leads to cell death and enhanced disease response in rice
title_fullStr Disruption of gene SPL35, encoding a novel CUE domain‐containing protein, leads to cell death and enhanced disease response in rice
title_full_unstemmed Disruption of gene SPL35, encoding a novel CUE domain‐containing protein, leads to cell death and enhanced disease response in rice
title_short Disruption of gene SPL35, encoding a novel CUE domain‐containing protein, leads to cell death and enhanced disease response in rice
title_sort disruption of gene spl35, encoding a novel cue domain‐containing protein, leads to cell death and enhanced disease response in rice
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662554/
https://www.ncbi.nlm.nih.gov/pubmed/30771255
http://dx.doi.org/10.1111/pbi.13093
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