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Phylogeography of Pulsatilla cernua (Ranunculaceae), a grassland species, in Japan

The genetic diversity and structure of Pulsatilla cernua, a continental‐grassland relict, were investigated using variations in chloroplast DNA (cpDNA) and microsatellites of nuclear DNA. In the analyses of three cpDNA regions, 17 haplotypes were found in 24 populations of P. cernua from Japan, Kore...

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Autores principales: Takaishi, Asuka, Kozhevnikov, Andrey E., Kozhevnikova, Zoya V., Ikeda, Hajime, Fujii, Noriyuki, Soejima, Akiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662557/
https://www.ncbi.nlm.nih.gov/pubmed/31380048
http://dx.doi.org/10.1002/ece3.5298
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author Takaishi, Asuka
Kozhevnikov, Andrey E.
Kozhevnikova, Zoya V.
Ikeda, Hajime
Fujii, Noriyuki
Soejima, Akiko
author_facet Takaishi, Asuka
Kozhevnikov, Andrey E.
Kozhevnikova, Zoya V.
Ikeda, Hajime
Fujii, Noriyuki
Soejima, Akiko
author_sort Takaishi, Asuka
collection PubMed
description The genetic diversity and structure of Pulsatilla cernua, a continental‐grassland relict, were investigated using variations in chloroplast DNA (cpDNA) and microsatellites of nuclear DNA. In the analyses of three cpDNA regions, 17 haplotypes were found in 24 populations of P. cernua from Japan, Korea, and Russia. Although the route and time of migration between the continent of Asia and Japan could not be well resolved, the cpDNA haplotype network suggests the existence of several ancient lineages in Japan and a recent secondary migration from Japan to the continent. Microsatellite analyses did not indicate genetic structure among the Japanese populations, indicating the existence of gene flow across the distribution area until recently. These results indicate that the present fragmentation of P. cernua in Japan may reflect a rapid, recent reduction from a previously large, continuous distribution.
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spelling pubmed-66625572019-08-02 Phylogeography of Pulsatilla cernua (Ranunculaceae), a grassland species, in Japan Takaishi, Asuka Kozhevnikov, Andrey E. Kozhevnikova, Zoya V. Ikeda, Hajime Fujii, Noriyuki Soejima, Akiko Ecol Evol Original Research The genetic diversity and structure of Pulsatilla cernua, a continental‐grassland relict, were investigated using variations in chloroplast DNA (cpDNA) and microsatellites of nuclear DNA. In the analyses of three cpDNA regions, 17 haplotypes were found in 24 populations of P. cernua from Japan, Korea, and Russia. Although the route and time of migration between the continent of Asia and Japan could not be well resolved, the cpDNA haplotype network suggests the existence of several ancient lineages in Japan and a recent secondary migration from Japan to the continent. Microsatellite analyses did not indicate genetic structure among the Japanese populations, indicating the existence of gene flow across the distribution area until recently. These results indicate that the present fragmentation of P. cernua in Japan may reflect a rapid, recent reduction from a previously large, continuous distribution. John Wiley and Sons Inc. 2019-06-13 /pmc/articles/PMC6662557/ /pubmed/31380048 http://dx.doi.org/10.1002/ece3.5298 Text en © 2019 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Takaishi, Asuka
Kozhevnikov, Andrey E.
Kozhevnikova, Zoya V.
Ikeda, Hajime
Fujii, Noriyuki
Soejima, Akiko
Phylogeography of Pulsatilla cernua (Ranunculaceae), a grassland species, in Japan
title Phylogeography of Pulsatilla cernua (Ranunculaceae), a grassland species, in Japan
title_full Phylogeography of Pulsatilla cernua (Ranunculaceae), a grassland species, in Japan
title_fullStr Phylogeography of Pulsatilla cernua (Ranunculaceae), a grassland species, in Japan
title_full_unstemmed Phylogeography of Pulsatilla cernua (Ranunculaceae), a grassland species, in Japan
title_short Phylogeography of Pulsatilla cernua (Ranunculaceae), a grassland species, in Japan
title_sort phylogeography of pulsatilla cernua (ranunculaceae), a grassland species, in japan
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662557/
https://www.ncbi.nlm.nih.gov/pubmed/31380048
http://dx.doi.org/10.1002/ece3.5298
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