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High-throughput identification of peptide agonists against GPCRs by co-culture of mammalian reporter cells and peptide-secreting yeast cells using droplet microfluidics
Since G-protein coupled receptors (GPCRs) are linked to various diseases, screening of functional ligands against GPCRs is vital for drug discovery. In the present study, we developed a high-throughput functional cell-based assay by combining human culture cells producing a GPCR, yeast cells secreti...
Autores principales: | Yaginuma, Kenshi, Aoki, Wataru, Miura, Natsuko, Ohtani, Yuta, Aburaya, Shunsuke, Kogawa, Masato, Nishikawa, Yohei, Hosokawa, Masahito, Takeyama, Haruko, Ueda, Mitsuyoshi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662714/ https://www.ncbi.nlm.nih.gov/pubmed/31358824 http://dx.doi.org/10.1038/s41598-019-47388-x |
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