Generation of human induced pluripotent stem cells from individuals with a homozygous CCR5Δ32 mutation

Chemokine receptor 5 (CCR5) is the primary coreceptor for HIV entry into macrophages. Individuals with a homozygous deletion of 32 bp in the CCR5 gene (CCR5Δ32) are highly resistant to HIV infection (Samson et al., 1996). Allogeneic stem cell transplantation from a healthy donor with the homozygous...

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Autores principales: Chen, Guibin, Jin, Hui, Yu, Zhen, Liu, Yangtengyu, Li, Zhongwen, Navarengom, Keron, Schwartzbeck, Robin, Dmitrieva, Natalia, Cudrici, Cornelia, Ferrante, Elisa A., Biesecker, Leslie G., Yang, Dan, Boehm, Manfred
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662727/
https://www.ncbi.nlm.nih.gov/pubmed/31234109
http://dx.doi.org/10.1016/j.scr.2019.101481
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author Chen, Guibin
Jin, Hui
Yu, Zhen
Liu, Yangtengyu
Li, Zhongwen
Navarengom, Keron
Schwartzbeck, Robin
Dmitrieva, Natalia
Cudrici, Cornelia
Ferrante, Elisa A.
Biesecker, Leslie G.
Yang, Dan
Boehm, Manfred
author_facet Chen, Guibin
Jin, Hui
Yu, Zhen
Liu, Yangtengyu
Li, Zhongwen
Navarengom, Keron
Schwartzbeck, Robin
Dmitrieva, Natalia
Cudrici, Cornelia
Ferrante, Elisa A.
Biesecker, Leslie G.
Yang, Dan
Boehm, Manfred
author_sort Chen, Guibin
collection PubMed
description Chemokine receptor 5 (CCR5) is the primary coreceptor for HIV entry into macrophages. Individuals with a homozygous deletion of 32 bp in the CCR5 gene (CCR5Δ32) are highly resistant to HIV infection (Samson et al., 1996). Allogeneic stem cell transplantation from a healthy donor with the homozygous CCR5Δ32 variant to an HIV positive individual has demonstrated efficient long-term control of HIV. We identified three individuals with this homozygous CCR5Δ32 variant, and successfully generated induced pluripotent stem cell (iPSC) lines from their dermal fibroblasts. The iPSCs lines carrying homozygous CCR5Δ32 variant displayed phenotypically normal and the potential to differentiation toward the three germ layers.
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spelling pubmed-66627272019-07-29 Generation of human induced pluripotent stem cells from individuals with a homozygous CCR5Δ32 mutation Chen, Guibin Jin, Hui Yu, Zhen Liu, Yangtengyu Li, Zhongwen Navarengom, Keron Schwartzbeck, Robin Dmitrieva, Natalia Cudrici, Cornelia Ferrante, Elisa A. Biesecker, Leslie G. Yang, Dan Boehm, Manfred Stem Cell Res Article Chemokine receptor 5 (CCR5) is the primary coreceptor for HIV entry into macrophages. Individuals with a homozygous deletion of 32 bp in the CCR5 gene (CCR5Δ32) are highly resistant to HIV infection (Samson et al., 1996). Allogeneic stem cell transplantation from a healthy donor with the homozygous CCR5Δ32 variant to an HIV positive individual has demonstrated efficient long-term control of HIV. We identified three individuals with this homozygous CCR5Δ32 variant, and successfully generated induced pluripotent stem cell (iPSC) lines from their dermal fibroblasts. The iPSCs lines carrying homozygous CCR5Δ32 variant displayed phenotypically normal and the potential to differentiation toward the three germ layers. 2019-06-05 2019-07 /pmc/articles/PMC6662727/ /pubmed/31234109 http://dx.doi.org/10.1016/j.scr.2019.101481 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Chen, Guibin
Jin, Hui
Yu, Zhen
Liu, Yangtengyu
Li, Zhongwen
Navarengom, Keron
Schwartzbeck, Robin
Dmitrieva, Natalia
Cudrici, Cornelia
Ferrante, Elisa A.
Biesecker, Leslie G.
Yang, Dan
Boehm, Manfred
Generation of human induced pluripotent stem cells from individuals with a homozygous CCR5Δ32 mutation
title Generation of human induced pluripotent stem cells from individuals with a homozygous CCR5Δ32 mutation
title_full Generation of human induced pluripotent stem cells from individuals with a homozygous CCR5Δ32 mutation
title_fullStr Generation of human induced pluripotent stem cells from individuals with a homozygous CCR5Δ32 mutation
title_full_unstemmed Generation of human induced pluripotent stem cells from individuals with a homozygous CCR5Δ32 mutation
title_short Generation of human induced pluripotent stem cells from individuals with a homozygous CCR5Δ32 mutation
title_sort generation of human induced pluripotent stem cells from individuals with a homozygous ccr5δ32 mutation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662727/
https://www.ncbi.nlm.nih.gov/pubmed/31234109
http://dx.doi.org/10.1016/j.scr.2019.101481
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