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Particle coating alters mucociliary transit in excised rat trachea: A synchrotron X-ray imaging study
We have previously developed non-invasive in vivo mucociliary transport (MCT) monitoring methods using synchrotron phase contrast X-ray imaging (PCXI) to evaluate potential therapies for cystic fibrosis (CF). However, previous in vivo measurements of MCT velocity using this method were lower than th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662859/ https://www.ncbi.nlm.nih.gov/pubmed/31358851 http://dx.doi.org/10.1038/s41598-019-47465-1 |
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author | Gardner, Mark McCarron, Alexandra Morgan, Kaye Parsons, David Donnelley, Martin |
author_facet | Gardner, Mark McCarron, Alexandra Morgan, Kaye Parsons, David Donnelley, Martin |
author_sort | Gardner, Mark |
collection | PubMed |
description | We have previously developed non-invasive in vivo mucociliary transport (MCT) monitoring methods using synchrotron phase contrast X-ray imaging (PCXI) to evaluate potential therapies for cystic fibrosis (CF). However, previous in vivo measurements of MCT velocity using this method were lower than those from alternate methods. We hypothesise this was due to the surface chemistry of the uncoated particles. We investigated the effect of particle surface coating on MCT marker performance by measuring the velocity of uncoated, positively-charged (aminated; NH(2)), and negatively-charged (carboxylated; COOH) particles. The effect of aerosolised hypertonic saline (HS) was also investigated, as previous in vivo measurements showed HS significantly increased MCT rate. PCXI experiments were performed using an ex vivo rat tracheal imaging setup. Prior to aerosol delivery there was little movement of the uncoated particles, whilst the NH(2) and COOH particles moved with MCT rates similar to those previously reported. After application of HS the uncoated and COOH particle velocity increased and NH(2) decreased. This experiment validated the use of COOH particles as MCT marker particles over the uncoated and NH(2) coated particles. Our results suggest that future experiments measuring MCT using synchrotron PCXI should use COOH coated marker particles for more accurate MCT quantification. |
format | Online Article Text |
id | pubmed-6662859 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-66628592019-08-02 Particle coating alters mucociliary transit in excised rat trachea: A synchrotron X-ray imaging study Gardner, Mark McCarron, Alexandra Morgan, Kaye Parsons, David Donnelley, Martin Sci Rep Article We have previously developed non-invasive in vivo mucociliary transport (MCT) monitoring methods using synchrotron phase contrast X-ray imaging (PCXI) to evaluate potential therapies for cystic fibrosis (CF). However, previous in vivo measurements of MCT velocity using this method were lower than those from alternate methods. We hypothesise this was due to the surface chemistry of the uncoated particles. We investigated the effect of particle surface coating on MCT marker performance by measuring the velocity of uncoated, positively-charged (aminated; NH(2)), and negatively-charged (carboxylated; COOH) particles. The effect of aerosolised hypertonic saline (HS) was also investigated, as previous in vivo measurements showed HS significantly increased MCT rate. PCXI experiments were performed using an ex vivo rat tracheal imaging setup. Prior to aerosol delivery there was little movement of the uncoated particles, whilst the NH(2) and COOH particles moved with MCT rates similar to those previously reported. After application of HS the uncoated and COOH particle velocity increased and NH(2) decreased. This experiment validated the use of COOH particles as MCT marker particles over the uncoated and NH(2) coated particles. Our results suggest that future experiments measuring MCT using synchrotron PCXI should use COOH coated marker particles for more accurate MCT quantification. Nature Publishing Group UK 2019-07-29 /pmc/articles/PMC6662859/ /pubmed/31358851 http://dx.doi.org/10.1038/s41598-019-47465-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Gardner, Mark McCarron, Alexandra Morgan, Kaye Parsons, David Donnelley, Martin Particle coating alters mucociliary transit in excised rat trachea: A synchrotron X-ray imaging study |
title | Particle coating alters mucociliary transit in excised rat trachea: A synchrotron X-ray imaging study |
title_full | Particle coating alters mucociliary transit in excised rat trachea: A synchrotron X-ray imaging study |
title_fullStr | Particle coating alters mucociliary transit in excised rat trachea: A synchrotron X-ray imaging study |
title_full_unstemmed | Particle coating alters mucociliary transit in excised rat trachea: A synchrotron X-ray imaging study |
title_short | Particle coating alters mucociliary transit in excised rat trachea: A synchrotron X-ray imaging study |
title_sort | particle coating alters mucociliary transit in excised rat trachea: a synchrotron x-ray imaging study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662859/ https://www.ncbi.nlm.nih.gov/pubmed/31358851 http://dx.doi.org/10.1038/s41598-019-47465-1 |
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