Characterization of PAN-1, a Carbapenem-Hydrolyzing Class B β-Lactamase From the Environmental Gram-Negative Pseudobacteriovorax antillogorgiicola

The gene encoding the metallo-β-lactamase (MβL) PAN-1 was identified in the genome of the environmental Gram-negative species Pseudobacteriovorax antillogorgiicola. PAN-1 shares 57% amino-acid identity with the acquired MβL SPM-1, its closest relative. Kinetic parameters performed on purified PAN-1...

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Autores principales: Kieffer, Nicolas, Poirel, Laurent, Fournier, Claudine, Haltli, Brad, Kerr, Russel, Nordmann, Patrice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6663966/
https://www.ncbi.nlm.nih.gov/pubmed/31396187
http://dx.doi.org/10.3389/fmicb.2019.01673
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author Kieffer, Nicolas
Poirel, Laurent
Fournier, Claudine
Haltli, Brad
Kerr, Russel
Nordmann, Patrice
author_facet Kieffer, Nicolas
Poirel, Laurent
Fournier, Claudine
Haltli, Brad
Kerr, Russel
Nordmann, Patrice
author_sort Kieffer, Nicolas
collection PubMed
description The gene encoding the metallo-β-lactamase (MβL) PAN-1 was identified in the genome of the environmental Gram-negative species Pseudobacteriovorax antillogorgiicola. PAN-1 shares 57% amino-acid identity with the acquired MβL SPM-1, its closest relative. Kinetic parameters performed on purified PAN-1 showed it displayed a hydrolytic activity toward most β-lactams including carbapenems but spared cefepime and aztreonam. These results further highlight that environmental bacterial species may be reservoirs of MβL encoding genes.
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spelling pubmed-66639662019-08-08 Characterization of PAN-1, a Carbapenem-Hydrolyzing Class B β-Lactamase From the Environmental Gram-Negative Pseudobacteriovorax antillogorgiicola Kieffer, Nicolas Poirel, Laurent Fournier, Claudine Haltli, Brad Kerr, Russel Nordmann, Patrice Front Microbiol Microbiology The gene encoding the metallo-β-lactamase (MβL) PAN-1 was identified in the genome of the environmental Gram-negative species Pseudobacteriovorax antillogorgiicola. PAN-1 shares 57% amino-acid identity with the acquired MβL SPM-1, its closest relative. Kinetic parameters performed on purified PAN-1 showed it displayed a hydrolytic activity toward most β-lactams including carbapenems but spared cefepime and aztreonam. These results further highlight that environmental bacterial species may be reservoirs of MβL encoding genes. Frontiers Media S.A. 2019-07-23 /pmc/articles/PMC6663966/ /pubmed/31396187 http://dx.doi.org/10.3389/fmicb.2019.01673 Text en Copyright © 2019 Kieffer, Poirel, Fournier, Haltli, Kerr and Nordmann. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Kieffer, Nicolas
Poirel, Laurent
Fournier, Claudine
Haltli, Brad
Kerr, Russel
Nordmann, Patrice
Characterization of PAN-1, a Carbapenem-Hydrolyzing Class B β-Lactamase From the Environmental Gram-Negative Pseudobacteriovorax antillogorgiicola
title Characterization of PAN-1, a Carbapenem-Hydrolyzing Class B β-Lactamase From the Environmental Gram-Negative Pseudobacteriovorax antillogorgiicola
title_full Characterization of PAN-1, a Carbapenem-Hydrolyzing Class B β-Lactamase From the Environmental Gram-Negative Pseudobacteriovorax antillogorgiicola
title_fullStr Characterization of PAN-1, a Carbapenem-Hydrolyzing Class B β-Lactamase From the Environmental Gram-Negative Pseudobacteriovorax antillogorgiicola
title_full_unstemmed Characterization of PAN-1, a Carbapenem-Hydrolyzing Class B β-Lactamase From the Environmental Gram-Negative Pseudobacteriovorax antillogorgiicola
title_short Characterization of PAN-1, a Carbapenem-Hydrolyzing Class B β-Lactamase From the Environmental Gram-Negative Pseudobacteriovorax antillogorgiicola
title_sort characterization of pan-1, a carbapenem-hydrolyzing class b β-lactamase from the environmental gram-negative pseudobacteriovorax antillogorgiicola
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6663966/
https://www.ncbi.nlm.nih.gov/pubmed/31396187
http://dx.doi.org/10.3389/fmicb.2019.01673
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