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Bacterial Cheaters Evade Punishment by Cyanide
In all domains of life, mechanisms exist that protect cooperating groups from exploitation by cheaters. Recent observations with the bacterium Pseudomonas aeruginosa have suggested a paradigmatic cheater control mechanism in which cooperator cells punish or “police” cheater cells by cyanide poisonin...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6664145/ https://www.ncbi.nlm.nih.gov/pubmed/31357168 http://dx.doi.org/10.1016/j.isci.2019.07.015 |
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author | Smith, Parker Cozart, Jamison Lynn, Bryan K. Alberts, Erin Frangipani, Emanuela Schuster, Martin |
author_facet | Smith, Parker Cozart, Jamison Lynn, Bryan K. Alberts, Erin Frangipani, Emanuela Schuster, Martin |
author_sort | Smith, Parker |
collection | PubMed |
description | In all domains of life, mechanisms exist that protect cooperating groups from exploitation by cheaters. Recent observations with the bacterium Pseudomonas aeruginosa have suggested a paradigmatic cheater control mechanism in which cooperator cells punish or “police” cheater cells by cyanide poisoning. These cheater cells are deficient in a pleiotropic quorum-sensing regulator that controls the production of cooperative secretions including cyanide, and presumably also cyanide resistance. In this study, we directly tested and refuted the cyanide policing model. Contrary to the hypothesis, cheater fitness was unaffected by the presence of cyanide. Cheater mutants grew equally well in co-cultures with either cyanide-proficient or cyanide-deficient cooperators, and they were as resistant to exogenous cyanide as wild-type cells. We show that these behaviors are the result of quorum-sensing-independent and cyanide-responsive resistance gene regulation. Our results highlight the role of genetic architecture in the evolution of cooperative behavior. |
format | Online Article Text |
id | pubmed-6664145 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-66641452019-08-05 Bacterial Cheaters Evade Punishment by Cyanide Smith, Parker Cozart, Jamison Lynn, Bryan K. Alberts, Erin Frangipani, Emanuela Schuster, Martin iScience Article In all domains of life, mechanisms exist that protect cooperating groups from exploitation by cheaters. Recent observations with the bacterium Pseudomonas aeruginosa have suggested a paradigmatic cheater control mechanism in which cooperator cells punish or “police” cheater cells by cyanide poisoning. These cheater cells are deficient in a pleiotropic quorum-sensing regulator that controls the production of cooperative secretions including cyanide, and presumably also cyanide resistance. In this study, we directly tested and refuted the cyanide policing model. Contrary to the hypothesis, cheater fitness was unaffected by the presence of cyanide. Cheater mutants grew equally well in co-cultures with either cyanide-proficient or cyanide-deficient cooperators, and they were as resistant to exogenous cyanide as wild-type cells. We show that these behaviors are the result of quorum-sensing-independent and cyanide-responsive resistance gene regulation. Our results highlight the role of genetic architecture in the evolution of cooperative behavior. Elsevier 2019-07-16 /pmc/articles/PMC6664145/ /pubmed/31357168 http://dx.doi.org/10.1016/j.isci.2019.07.015 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Smith, Parker Cozart, Jamison Lynn, Bryan K. Alberts, Erin Frangipani, Emanuela Schuster, Martin Bacterial Cheaters Evade Punishment by Cyanide |
title | Bacterial Cheaters Evade Punishment by Cyanide |
title_full | Bacterial Cheaters Evade Punishment by Cyanide |
title_fullStr | Bacterial Cheaters Evade Punishment by Cyanide |
title_full_unstemmed | Bacterial Cheaters Evade Punishment by Cyanide |
title_short | Bacterial Cheaters Evade Punishment by Cyanide |
title_sort | bacterial cheaters evade punishment by cyanide |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6664145/ https://www.ncbi.nlm.nih.gov/pubmed/31357168 http://dx.doi.org/10.1016/j.isci.2019.07.015 |
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